This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
CRYAB antibody (M03496) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
IHC analysis of Alpha B Crystallin/CRYAB using anti-Alpha B Crystallin/CRYAB antibody (M03496).
Alpha B Crystallin/CRYAB was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Alpha B Crystallin/CRYAB Antibody (M03496) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Alpha B Crystallin/CRYAB was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Alpha B Crystallin/CRYAB Antibody (M03496) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Bundle Save
Anti-CRYAB/Alpha B Crystallin Rabbit Monoclonal Antibody
CRYAB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
CRYAB antibody was positively detected by WB in:
rat eye tissue, rat heart tissue, mouse eye tissue, mouse heart tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CRYAB antibody was positively detected by IHC in:
human kidney tissue, human renal cancer tissue, rat heart tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
ICC
IF
IP
Rat
Tested
Tested
Recommend
Recommend
Recommend
Mouse
Tested
Human
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-CRYAB/Alpha B Crystallin Rabbit Monoclonal Antibody
View all CRYAB Antibodies
View all CRYAB Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABHC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CRYAB
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
22 kDa
Calculated Molecular Weight
20.2 kDa
Cite This Product
Anti-CRYAB/Alpha B Crystallin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03496)
Protocols
Western Blot protocol for CRYAB antibody
Immunohistochemistry (IHC) protocol for CRYAB antibody
Review
Have you used Anti-CRYAB/Alpha B Crystallin Rabbit Monoclonal Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-CRYAB/Alpha B Crystallin Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M03496
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026