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- Table of Contents
Facts about Endothelin-1 receptor.
Receptor for endothelin-1.
Mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system.The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3. .
Human | |
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Gene Name: | EDNRA |
Uniprot: | P25101 |
Entrez: | 1909 |
Belongs to: |
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G-protein coupled receptor 1 family |
EDNRA; endothelin receptor type A; endothelin-1 receptor; endothelin-1-specific receptor; ET1-specific type; ETAR; ETRA; G protein-coupled receptor; hET-AR
Mass (kDA):
48.722 kDA
Human | |
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Location: | 4q31.22-q31.23 |
Sequence: | 4; NC_000004.12 (147480917..147544954) |
Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.
Cell membrane; Multi-pass membrane protein.
In this article, we'll discuss Boster Bio's AntiEDNRA Marker and discuss the best uses of this marker in PCR and other research. This article also covers the validation process, PCR primers and more. The information contained in this article is applicable to all scientists, no matter the location of their lab. The Boster Bio Anti-PCNA Marker is a important tool for identifying antigens and genes in samples.
The Boster Bio Anti-Endothelin B Receptor/EDNRB Antibody is humanized antibody that binds the endothelin B receptor gene. This antibody reacts with Human, Mouse, Rat and rats' cells. It has been validated for use in immunohistochemistry. This antibody is part the Picoband(tm).
The EDNRA gene encodes the endothelin receptor type B (PCNA). It activates the phosphatidylinositol-calcium second messenger system and is localized to the short arm of human chromosome 20. It is comprised of six exons, and measures approximately 4,961bp in length. The activated PCNA controls sister chromatid cohesion during the S phase. It is abundantly expressed in human tissues, and is also present in patients suffering from inflammatory diseases and tumor cells.
Boster Bio Anti-Endothelin B Receptor/EDNRB Antibody is an antigen monoclonal antibody that interacts with human, mouse, rodents and worms. The product has been tested for specificity and affinity in Flow Cytometry and IHC-P. Boster Bio Anti-EDNRA Marker is safe and is available without BSA. A blocking peptide with a concentration of 1.0mg/ml is available to ensure optimal performance of antibodies. The cost of a blocking peptide depends on its length.
The confirmation of the EDNRA marker in the study of cancer cells indicates that this gene has an important regulatory function in the differentiation of M2 macrophages into TAMs. Additionally, EDNRA has been found to be linked to the WNT signaling pathway , as well as the PI3K/AKT signaling systems. This suggests that the presence of EDNRA could increase the risk of developing STAD or contribute to the progression of.
EDNRA expression is related to the type and number of tumors in a particular cohort. It is also associated with the histology of the tumor. The relationship between tumor cell EDNRA and overall survival was observed for the T stage, histological grades, TP53, and PIK3CA status. In addition, the association was also observed for the Kaplan-Meier survival plot, where higher EDNRA protein expression was linked to a higher risk of death.
These findings suggest that EDNRA is an important, independent biomarker for prognosis. Furthermore, EDNRA expression levels have been linked to the presence of immune cells in gastric cancer cells. EDNRA expression is associated with advanced STAD patients. This marker may be beneficial when paired with other strategies for immunotherapy. The presence of EDNRA in cancer cells could suggest a possible therapeutic target.
The EDNRA gene product was expressed in both POD and mGEC cell lines. The 2-DDCt technique was used to determine differential expression. The TCGA STAD dataset was used for evaluating the GAPDH and EDNRA gene expression levels. We used primers 5'-GGACCTG-3' and 3'-GTAGCCACAGAGATTGA-3' respectively.
The expression of this gene is in line with the classical immune checkpoints like CD8 and PD-1. Moreover, the expression of EDNRA in various tumor types is also linked to the expression levels of the other classical immune checkpoints, such as CD20. Further validation studies must be conducted to determine whether it has a positive impact on PD-1 therapeutic effectiveness. The study results will aid in the development of immunotherapy treatments for the patients with advanced STAD.
In this study, EDNRA protein expression was determined in the validation cohort in order to determine the prognostic significance of the marker. We also determined the OS distinctions between patients with low and high EDNRA staining levels in the H-SCORE cohort. A Wilcoxon logrank test was also utilized. The P-values in this study were less than 0.05. Similar studies are being conducted to determine the prognostic significance of EDNRA in the context of hematological cancers.
The table below shows the sequences of the primers used for the EDNRA marker. The primers were denaturated at 95degC for 5 min and then annealed between 52degC and 60degC for 45 seconds. The PCR products were then incubated for 7 minutes at 72degC. They were separated on the 2% agarose gels. They were then stained with Safer Ethidium Bromide Alternatives GelGreen. Images were captured by an Image Analyzer ChemiDoc XRS+.
Total RNA was extracted from a human SSC line cell as well as male germ cells taken from patients suffering from NOA or OA. The Nanodrop (Takara Kusatsu, Japan) was used to measure the RNA. The quality and concentration of RNA was checked with the Nanodrop. To ensure the highest quality the RNA concentration was set to 1.9-2.0 The First Strand cDNA synthesizer kit was used to synthesize the cDNA. The primer sequences are described in Table 4.
In mice, MCMV infection reduced expression of miR-1929-3p. It is an endothelial cell inflammatory protein that is a direct target of the Ednra molecule. miR-1929-3p blocks the translation of target genes. After MCMV infection miR-1929-3p has been linked to EH. The miR-1929-3p target, Ednra is located in the VSMCs and primarily participates in vasoconstriction and oxidative stress. Ednra is linked to endothelial injuries and activation of the inflammation pathway. Inhibitors of the EDNRA marker can reduce the negative effects.
PMID: 1719979 by Adachi M., et al. Cloning and characterization of cDNA encoding human A-type endothelin receptor.
PMID: 1659806 by Cyr C., et al. Cloning and chromosomal localization of a human endothelin ETA receptor.
*More publications can be found for each product on its corresponding product page