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- Table of Contents
EDNRA antibody (A01828-4) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Flow Cytometry analysis of A549 cells using anti-Endothelin A Receptor/ET-A/ EDNRA antibody (A01828-4).
Overlay histogram showing A549 cells stained with A01828-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Endothelin A Receptor/ET-A/ EDNRA Antibody (A01828-4,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A549 cells stained with A01828-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Endothelin A Receptor/ET-A/ EDNRA Antibody (A01828-4,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Endothelin A Receptor/ET-A/EDNRA Antibody Picoband®
EDNRA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
EDNRA antibody was positively detected by WB in:
human HEK293 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
EDNRA antibody was positively detected by IHC in:
human lung cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
EDNRA antibody was positively detected by FC in:
A549 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Endothelin A Receptor/ET-A/EDNRA Antibody Picoband®
View all EDNRA Antibodies
View all EDNRA Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Endothelin A Receptor/ET-A/EDNRA recombinant protein (Position: N32-Q381).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated Molecular Weight
48.7 kDa
Background of EDNRA
This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-Endothelin A Receptor/ET-A/EDNRA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01828-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Rabbit
97%
High Homology
Dog
97%
High Homology
Sheep
97%
High Homology
Cow
96%
High Homology
Rat
95%
High Homology
Mouse
95%
High Homology
Zebrafish
69%
Low homology
Drosophila
31%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for EDNRA antibody
Immunohistochemistry (IHC) protocol for EDNRA antibody
Flow Cytometry (FC) protocol for EDNRA antibody
Publications
Application:
Species:
Endothelin-1 potentiated constriction in preeclampsia placental veins: role of ETAR/ETBR/CaV1.2/CALD1
Published: 2024-10-24
Journal: PLACENTA
Application: WB
Impact Factor: 3.00
Species: Human
PMID: 39476475
The separate roles of endothelin receptors participate in remodeling of matrix metalloproteinase and connexin 43 of cardiac fibroblasts in maladaptive response to isoproterenol
Published: 2010-02-16
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 2.59
Species: Rat
PMID: 20167215
Normalizing NADPH Oxidase Contributes to Attenuating Diabetic Nephropathy by the Dual Endothelin Receptor Antagonist CPU0213 in Rats
Published: 2008-09-19
Journal: AMERICAN JOURNAL OF NEPHROLOGY
Application: WB
Impact Factor: 2.18
Species: Rat
PMID: 18802327
EFFECT OF THE ENDOTHELIN RECEPTOR ANTAGONIST CPU0213, AND ITS MODULATION BY RIFAMPIN, ON CARDIAC AND VASCULAR TISSUE FOLLOWING CHRONIC ISOPROTERENOL TREATMENT
Published: 2008-01-21
Journal: CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
Application: WB
Impact Factor: 2.04
Species: Rat
PMID: 18215179
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Product Promise
We promise Anti-Endothelin A Receptor/ET-A/EDNRA Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01828-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026