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- Table of Contents
Facts about Eukaryotic translation initiation factor 3 subunit B.
The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and then prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:9388245, PubMed:17581632).
Human | |
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Gene Name: | EIF3B |
Uniprot: | P55884 |
Entrez: | 8662 |
Belongs to: |
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eIF-3 subunit B family |
eIF3 p110; eIF3 p116; eIF3b; eIF-3-eta; EIF3-P110; EIF3-P116; EIF3S9EIF3-ETA; Eukaryotic translation initiation factor 3 subunit 9; eukaryotic translation initiation factor 3 subunit B; eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD); eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa; eukaryotic translation initiation factor 3, subunit B; MGC104664; MGC131875; Prt1 homolog; PRT1hPrt1
Mass (kDA):
92.482 kDA
Human | |
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Location: | 7p22.3 |
Sequence: | 7; NC_000007.14 (2354086..2380745) |
Cytoplasm.
If you're interested in developing a biomarker to detect EIF3B, you should learn more about Steven Boster. This article will discuss Boster's biomarker, the high-affinity prima antibodies, and their applications. Read on for more information! You can also check his references and biochemical data. Boster Bio: Best Uses of The EIF3BMarker
For cancer research, high-affinity primary antibodies against EIF3B are now available. This protein plays an important role in the regulation and death of cells. Researchers found that the protein was abundant in tumor cells. This may indicate its importance in HNSCC. The study also identified other cell-death pathways, such ferroptosis as necrosis. This suggests that EIF3B acts as a regulator of HNSCC-related cell proliferation and death.
A mutation in eIF5's enzyme impairs its ability, in vivo, to link with eIF2 & eIF3. This mutation decreases the interaction with eIF3 & MFC. However the association to HA-eIF3 has been enhanced. The high-affinity antibodies that target eIF3B are characterized by a unique epitope recognized motif that binds eIF3B directly to its subunit.
The indicated drugs and EIF3B suppressed Fadu cells were then incubated. The cells were then washed twice with PBS, fixed with paraformaldehyde for 15 min, and permeabilized with cold methanol for 10 min. After incubating the cells for the required time, they were blocked in 5% normal mare serum in PBS. High-affinity antibodies can only be produced using iRT calibration and gradient stabilization. The FDR cutoff was set at 1% at the precursor level, and decoy generation was performed to use random amino acid positions swamps. Finally, selected fragments could be quantified using an ECL detector system.
The EIF3B marker is now available for use in different types of cancer research. The lentivirus mediated eIF3B RNA-delivery system was created to accomplish this. The EIF3B RNA could be delivered into cells using a lentivirus containing hU6–MCS–ubiquitin EGFP IRES, puromycin, IRES, puromycin, EGFP, and a 5 ug/mL polybrene. The clones of the EIF3B siRNA were then transfected by Enhanced Infection Solution (or 5 ug/mL) respectively. The EIF3B-siRNA was then transferred to the clones. They were then cultured in normal media with 10% (v/v), plus polybrene.
Several human cancers over-express eIF3B, making it an important tumor biomarker. It is an important factor in invasion and metastasis. Wang et.al. Wang et.al. discovered that eIF3b promotes lung metastasis as well as growth in prostate and bladder cancers. Its role is not yet clear in melanoma. EIF3B markers are being used in cancer research.
The TCGA SKCM data set was analysed to determine the most effective treatment for EIF3B-expressing tumor cells. The resulting enriched pathways were calculated using GSVA and ssGSEA analyses. Pearson correlation tests were used by to estimate correlation coefficients. LASSO modeling were trained using a 10-fold crossover approach. The submap analysis in the TCGA SKCM dataset reveals a lower EIF3B class in the GEO population, but the patients remain susceptible to ICB treatments.
The eIF3 molecule plays an important part in mRNA-binding at the entry and exhaust channels of cells. Mutants of eIF3b bind mRNA at positions 8-17, which stabilizes the interaction between the mRNA & the PIC. This enzyme is involved in the progression malignant diseases like leukemia, lymphoma, and other forms of cancer. This protein is involved at various stages of mRNA translation, including those involved with cell proliferation and differentiation.
The EIF3B mark is a gene that has its expression levels controlled by many factors. It is also known to be the O-GlcNAcylation target20, and is essential for ribosome activate. In addition to this, it is a scaffold protein for the JNK and SAPK pathways. Despite its importance however, eIF3B's function is not well understood.
PMID: 9388245 by Chaudhuri J., et al. Biochemical characterization of mammalian translation initiation factor 3 (eIF3). Molecular cloning reveals that p110 subunit is the mammalian homologue of Saccharomyces cerevisiae protein Prt1.
PMID: 8995410 by Methot N., et al. The human homologue of the yeast Prt1 protein is an integral part of the eukaryotic initiation factor 3 complex and interacts with p170.