|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Eif3b Antibody|
|Description||Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 3 subunit B(EIF3B) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-Eif3b Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2030)|
|Replacement Item||This antibody may replace the following items: sc-137214|sc-137215|sc-16377|sc-16378|sc-28857 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human eIF3B(755-775aa EDFRKYRKMAQELYMEQKNER), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Eukaryotic translation initiation factor 3 subunit B|
|Molecular Weight||92482 MW|
|Protein Function||Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre- initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. .|
|Sequence Similarities||Belongs to the eIF-3 subunit B family.|
|Subcellular Localization||Cytoplasm .|
|Alternative Names||Eukaryotic translation initiation factor 3 subunit B ;eIF3b ;Eukaryotic translation initiation factor 3 subunit 9 ;Prt1 homolog;hPrt1;eIF-3-eta ;eIF3 p110 ;eIF3 p116;EIF3B ;EIF3S9 ;|
|Research Areas|||epigenetics and nuclear signaling|dna / rna|translation|regulation||
Background for Eukaryotic translation initiation factor 3 subunit B
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Eif3b Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Eif3b Antibody Images
Click the images to enlarge.
Lane 1: HELA Cell Lysate
Lane 2: 293T Cell Lysate
Lane 3: A431 Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,