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Facts about Eukaryotic translation initiation factor 3 subunit E.
The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and then prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF- 3 complex especially targets and initiates translation of a subset of mRNAs involved in cell proliferation, including mobile cycling, differentiation and apoptosis, and uses different styles of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
Human | |
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Gene Name: | EIF3E |
Uniprot: | P60228 |
Entrez: | 3646 |
Belongs to: |
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eIF-3 subunit E family |
eIF-3 p48; eIF3e; eIF3-p48; EIF3S6EIF3-P48; Eukaryotic translation initiation factor 3 subunit 6; eukaryotic translation initiation factor 3 subunit E; eukaryotic translation initiation factor 3, subunit 6 (48kD); eukaryotic translation initiation factor 3, subunit 6 48kDa; eukaryotic translation initiation factor 3, subunit E; INT6eIF3-p46; mammary tumor-associated protein INT6; murine mammary tumor integration site 6 (oncogene homolog); Viral integration site protein INT-6 homolog
Mass (kDA):
52.221 kDA
Human | |
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Location: | 8q23.1 |
Sequence: | 8; NC_000008.11 (108201216..108248734, complement) |
Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus.
Cytoplasm. Nucleus, PML body.
Boster provides high-affinity primaries to the eIF-3 compound. The antibodies are created from 13 subunits of bovine Thymus fibrblast cells. Steven Boster, the founder of Boster Bio, is a biochemist who has developed high-sensitivity ELISA kits.
The eIF-3 complex is made up of 13 subunits and was first observed in human cells. Trypanosomatids have a near-complete eIF3 complex. Trypanosomabrucei, the most common trypanosome, and Leishmania major both have distinct eIF3 units that are very like the human eIF3 system. G. duodenalis also has an eIF3c as well as T. vaginalis is an subunit of the eIF3d.
In vivo reconstitution studies on mammalian eIF3 revealed that two subunits, eIF3f, and eIF3h are vital for eIF3 function. They also have functional connections. Three of the conserved subunits (eIF3f and eIF3h) are more like signalosome subunit Csn6 than they are to the proteasome subunit Rpn11.
eIF3b which is also known as PRT1, is one of the largest scaffolding proteins. It also has an uncanonical RRM Domain that functions as a platform for protein interactions. Its WD repeat domain which has nine bladed B-propellers, is in contact with the 40S subunit of the ribosome. L. major and T. the brucei are homologues of the human eIF3b protein.
M. jannaschii is the only eukaryote that has homologues to eIF3i. The WD ortholog shows an enticing relationship. Both proteins are alike in size and shape and connect with the eIF3i subunit. Conservation is greater close to the N-terminal edges of the eIF3i Subunits. M. jannaschii homologues and WD homologues differ in certain aspects.
The eIF3 function is mediated by the three eIF3 subunits. Cross-linking to mRNA can be achieved at positions -8, -17 for the most common mammalian subunits eIF3e and eIF3f. This allows for mRNA stability within the PIC. This is referred to as eIF3b-associated complex.
Despite the high degree of evolutionary conservation, little is known about eIF3 function in trypanosomes. The eIF-3 complex is found across all lineages of eukaryotic evolution, however, some subunits have distinctive characteristics that permit them to carry out distinct functions. The structure of eIF3 is the same across all eukaryotes however the variety of the translation apparatus in trypanosomes makes it stand out.
The largest subunit of eIF-3 (eIF3a) includes the PCI domain, as well as an orthologue, eIF3f. The human protein is an unconserved segment that includes the Nuclear Export Signal and is shared by all trypanosomatids. Although they have similar functions for eIF-3, they are different in their approach to controlling gene transcription.
eIF3a and eIF3b are homologous to human eIF3a. However their amino acid sequences share the lowest level of conservation with human proteins. The eIF3b, eIF3l proteins have similar sizes and features, and overall conservation is comparable to other eukaryotes. Its role in the cell's metabolic process is not understood, however.
For high-quality, reproducible results, Boster Bio offers rabbit mAbs for the EIF3E marker. This antibody has been tested in Flow Cymetry as well as ELISA, and is part Boster's Picoband(tm). It reacts with Human, Mouse, Rat and Yeast. Boster also offers an anti-rat antibody.
Primary antibodies are immunoglobulins that have a specific antigen that can be used in a variety of tests and tests. Primary antibodies are typically characterized by their specificity and affinity. A higher affinity indicates that the antibody is more likely to be able to bind to the target protein, whereas a lower specificity could be a sign of a weaker antibody. The best antibodies can be used for purification, detection, and measurement.
Steve Boster died on June 6, 2022. He was born in Joliet (IL). He was a long-time supervisor in sales for retail and a veteran of the U.S. Army. He is also survived by his two daughters, Natosha and Crystal Boster. and Crystal Boster. Other family members include his brother Donald Jr. and two sisters, Kimberly and Tammy, and his son Jonathan. He also has an extended family, which includes a number of nieces and nephews.
As father of two sons, Steve loved southern gospel music and was proud to perform it in private and public. He also loved sports, and especially his favourite opposing teams. He was also an avid driver. He often went to races at the local track even in the midst of sub-zero temperatures. Steve was generous to his family and friends and treated them as his family. If you have any questions or concerns about Steven Boster, don't hesitate to contact us. We're happy to assist you.
PMID: 9295280 by Asano K., et al. The translation initiation factor eIF3-p48 subunit is encoded by int- 6, a site of frequent integration by the mouse mammary tumor virus genome.
PMID: 9403073 by Miyazaki S., et al. The chromosome location of the human homolog of the mouse mammary tumor-associated gene INT6 and its status in human breast carcinomas.