Overview
| Product Name |
Anti-EIF3e Antibody Picoband™
See more EIF3E products |
| Catalog# |
A00481-1 |
| Pack Size |
100μg/vial |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-EIF3e Antibody Picoband™ catalog # A00481-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody. |
| Cite This Product |
Anti-EIF3e Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00481-1)
|
| Antibodies Validation |
Antibodies Validation Information
|
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Anti-EIF3e Antibody Picoband™ may replace the following items: sc 31746|sc 376109|sc 376110|sc 28859|sc 376821|sc 376899|sc 16370|sc 74505|sc 133251|sc 74506. |
Product Specs
| Host |
Rabbit |
| Reactive Species |
Human, Mouse, Rat |
| Applications |
WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $100. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
E.coli-derived human EIF3e recombinant protein (Position: A160-Q241). Human EIF3e shares 100% amino acid (aa) sequence identity with both mouse and rat EIF3e. |
| Cross Reactivity |
No cross reactivity with other proteins. |
Gene/Protein Basic Information For EIF3E (Source: Uniprot.org, NCBI)
| Uniprot Id | P60228 |
|---|
| NCBI Gene Id | 3646 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | EIF3E |
|---|
| Protein Name | Eukaryotic translation initiation factor 3 subunit E |
|---|
| Superfamily | eIF-3 subunit E family |
|---|
| Alternative Names | EIF3E|eIF-3 p48; eIF3e; eIF3-p48; EIF3S6EIF3-P48; Eukaryotic translation initiation factor 3 subunit 6; eukaryotic translation initiation factor 3 subunit E; eukaryotic translation initiation factor 3, subunit 6 (48kD); eukaryotic translation initiation factor 3, subunit 6 48kDa; eukaryotic translation initiation factor 3, subunit E; INT6eIF3-p46; mammary tumor-associated protein INT6; murine mammary tumor integration site 6 (oncogene homolog); Viral integration site protein INT-6 homolog |
|---|
| Molecular Weight | 52221 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on EIF3E, check out the EIF3E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for EIF3E: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the EIF3E infographic
Our Boster Quality Guarantee for Anti-EIF3e Antibody Picoband™ covers its use in the following applications.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 1. Western blot analysis of EIF3e using anti-EIF3e antibody (A00481-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: COLO320 whole cell lysates,
Lane 5: HELA whole Cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3e antigen affinity purified polyclonal antibody (Catalog # A00481-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF3e at approximately 52KD. The expected band size for EIF3e is at 52KD.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Questions and answers from customers related to A00481-1 Anti-EIF3e Antibody Picoband™
1 Related Questions
Question
We are currently using anti-EIF3e antibody A00481-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Asked: 2020-03-31
Answer
The anti-EIF3e antibody (A00481-1) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-31
