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- Table of Contents
ELAVL1 / ELAV-like protein 1 · Western blot design guide
Design a Western Blot for ELAVL1
Real validated ELAVL1 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-ELAVL1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
ELAVL1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~36.1 kDa | |
| Observed band | ~36 kDa | |
| Gel | 8% (catalog A00736-1) | |
| Positive control | Lung (IHC candidate; verify WB) +4 more | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 2 isoform(s) |
Section 1
Real Curated ELAVL1 Western Blot Protocols
The A00736-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human A431, human 293T, human Jurkat (catalog A00736-1) |
| Gel % | 8% (catalog A00736-1) |
| Load | 30 ug; reducing conditions (catalog A00736-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A00736-1) |
| Membrane | nitrocellulose membrane (catalog A00736-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A00736-1) |
| Primary antibody | A00736-1 · 1:1000 (catalog A00736-1) |
| Primary incubation | overnight at 4°C (catalog A00736-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A00736-1) |
| Secondary incubation | 1.5 hour at RT (catalog A00736-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A00736-1) |
| Detection | ECL (catalog A00736-1) |
Section 2
What Is the Expected ELAVL1 Western Blot Band Size?
ELAVL1 is predicted at 36.1 kDa and observed at ~36 kDa; the small apparent difference has no established cause.
| Band at ~36 kDa | Matches the observed ELAVL1 band and its 36.1 kDa predicted mass |
| Band near twice the monomer size | Could reflect the in vitro homodimer if it survives sample preparation |
| Several bands near the expected region | Could include isoforms 1 and 2, though distinct migration is unproven |
| Single band without an isoform doublet | Isoforms 1 and 2 may not resolve separately |
💡Expected ELAVL1 appearanceELAVL1 has a predicted mass of 36.1 kDa and an observed band at ~36 kDa; confirm band identity with an appropriate positive control and an independent antibody or knockdown.
| Predicted ELAVL1 mass | Places the monomer near 36.1 kDa, consistent with the ~36 kDa observed band |
| In vitro homodimer | Could appear near twice the monomer size if preserved during preparation |
| Isoform 1 | May differ in size from isoform 2; its separate mass and migration are unknown |
| Isoform 2 | May differ in size from isoform 1; its separate mass and migration are unknown |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | ELAVL1 signal may be below detection in the sampled lysate | Check a known positive lysate and compare nuclear and cytoplasmic fractions |
| Band higher than expected | The in vitro homodimer may persist during preparation | Compare strongly denatured samples and confirm identity by knockdown |
| Band lower than expected | Band identity is uncertain because isoform masses are unavailable | Check with an independent antibody or knockdown |
| Multiple bands | Isoforms 1 and 2 or preserved homodimer may contribute | Compare preparation conditions and identify ELAVL1 bands by knockdown |
| Weak or no signal | Signal may vary with ELAVL1 distribution between nucleus and cytoplasm | Check a positive lysate and assess both fractions |
Experiment evidence
Western blot images showing ELAVL1 band
A00736-1 · ELAVL1
M00736 · ELAVL1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for ELAVL1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for ELAVL1 in Western blot, you can use lung tissue lysate, which HPA rates as High.
Positive control: Lung (IHC candidate; verify WB)
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: HPA lists no tissue as not detected, so use siRNA knockdown or a KO line for a clean negative control.
HPA tissue expression evidence for ELAVL1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Lung | alveolar cells | High | Protein (IHC) | HPA → |
| Parathyroid gland | glandular cells | High | Protein (IHC) | HPA → |
| Rectum | glandular cells | High | Protein (IHC) | HPA → |
| Adipose tissue | adipocytes | Medium | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Liver | cholangiocytes | Low | Protein (IHC) | HPA → |
| Lymph node | germinal center cells | Low | Protein (IHC) | HPA → |
| Prostate | glandular cells | Low | Protein (IHC) | HPA → |
| Salivary gland | glandular cells | Low | Protein (IHC) | HPA → |
| Skeletal muscle | myocytes | Low | Protein (IHC) | HPA → |
Section 3
Advanced ELAVL1 Western Blot Tips
Deeper troubleshooting and optimisation questions for ELAVL1, answered from its protein features.
How should ELAVL1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could ELAVL1 isoforms produce different bands?
Isoforms · UniProt lists isoforms 1 and 2. Isoform 2 replaces the initial M with MGSGGRSAQVSTGQRAWLLPCRFLKNTM, changing its N terminus. Check which isoform your antibody recognizes before assigning a second band.
Which ELAVL1 phosphorylation sites matter when interpreting bands?
PTM · UniProt lists phosphoserine at positions 2, 100, 158, 197, 202, 221, and 318. These are UniProt coordinates; antibody or paper numbering may differ. Their presence alone does not establish a visible band shift.
The supplied UniProt features list no glycosylation sites or signal peptide. They provide no basis for assigning an unexpected band to glycosylated ELAVL1. Consider the listed isoform and modifications, but verify band identity experimentally.
The supplied UniProt features list no glycosylation sites or signal peptide. They provide no basis for assigning an unexpected band to glycosylated ELAVL1. Consider the listed isoform and modifications, but verify band identity experimentally.
Does this guide establish induction of ELAVL1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for ELAVL1?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A00736-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should ELAVL1 localization affect Western blot quantitation?
Quantitation · ELAVL1 is listed in both nucleus and cytoplasm, including stress granules and P-bodies. For fractionated samples, quantify within comparable fractions and account for redistribution when interpreting changes in band intensity.
Why is the ELAVL1 band near 36 kDa?
Interpretation · The observed band is about 36 kDa, close to the predicted 36.1 kDa. The listed modifications do not establish a visible mobility shift or explain a mass difference.
UniProt lists omega-N-methylarginine at position 206. At position 217, it lists alternate omega-N-methylarginine and asymmetric dimethylarginine by CARM1. Positions use UniProt numbering. Do not assign a separate band to either modification without supporting evidence.
UniProt reports ELAVL1 as a monomer and homodimer in vitro. That makes dimerization a possibility to investigate, but does not establish that a higher band on a Western blot is a dimer. Compare its behavior with the approximately 36 kDa band.
UniProt lists omega-N-methylarginine at position 206. At position 217, it lists alternate omega-N-methylarginine and asymmetric dimethylarginine by CARM1. Positions use UniProt numbering. Do not assign a separate band to either modification without supporting evidence.
UniProt reports ELAVL1 as a monomer and homodimer in vitro. That makes dimerization a possibility to investigate, but does not establish that a higher band on a Western blot is a dimer. Compare its behavior with the approximately 36 kDa band.
Boster reagents
ELAVL1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-HuR/ELAVL1 Antibody Picoband®
Cat # A00736-1
Real WB data
Anti-HuR / ELAVL1 Monoclonal Antibody
Cat # M00736
Both the supplier anti-ELAVL1 antibodies have Western blot images reporting a band near 36 kDa under reducing conditions. The captions show human cell and rat and mouse tissue lysates; no publication evidence was supplied. These examples do not establish performance in every specimen.
Which to pick: Choose A00736-1 for its listed human reactivity and illustrated human cell lysates; its image also includes rat and mouse brain. Choose M00736 when listed human, mouse, and rat reactivity matters; its image shows human cells and rat and mouse heart and spleen.
Source: BosterBio ELAVL1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q15717.
- Human Protein Atlas. ELAVL1 tissue expression.
- PMC13110253 — target-verified WB comparison
- PMC9225222 — target-verified WB comparison
- PMC13441531 — target-verified WB comparison