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- Table of Contents
ELAVL1 antibody (A00736-1) performs well in ELISA, Flow Cytometry, IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of A431 cells using anti-HuR/ELAVL1 antibody (A00736-1).
Overlay histogram showing A431 cells stained with A00736-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HuR/ELAVL1 Antibody (A00736-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A431 cells stained with A00736-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HuR/ELAVL1 Antibody (A00736-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-HuR/ELAVL1 Antibody Picoband®
ELAVL1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, ICC, WB |
Tested Applications
ELAVL1 antibody was positively detected by WB in:
human A431 whole cell, human 293T whole cell, human Jurkat whole cell, human HEL whole cell, rat brain tissue, mouse brain tissue, human Hela whole cell, normal rat colon tissue, rat colon cancer model tissue, human A549- WT whole cell lysates, human A549-ELAVL1 KO whole cell lysates
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ELAVL1 antibody was positively detected by IHC in:
human breast cancer tissue, human ovarian cancer tissue, mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ELAVL1 antibody was positively detected by ICC/IF in:
Hela cell
ELAVL1 antibody was positively detected by FC in:
A431 cell, Jurkat cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-HuR/ELAVL1 Antibody Picoband®
View all ELAVL1 Antibodies
View all ELAVL1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human HuR/ELAVL1 recombinant protein (Position: M1-S232).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
36 kDa
Calculated Molecular Weight
36.1 kDa
Background of ELAVL1
The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.
Cite This Product
Anti-HuR/ELAVL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00736-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
100%
High Homology
Cow
99%
High Homology
Sheep
99%
High Homology
Rabbit
99%
High Homology
Mouse
98%
High Homology
Rat
98%
High Homology
Zebrafish
81%
Moderate Homology
Drosophila
60%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ELAVL1 antibody
Immunohistochemistry (IHC) protocol for ELAVL1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ELAVL1 antibody
Flow Cytometry (FC) protocol for ELAVL1 antibody
Review
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1 Review For Anti-HuR/ELAVL1 Antibody Picoband®
The ELAVL1 antibody (A00736-1) produced a clear specific band at the expected size in WB using rat colon and colon cancer tissue samples, consistent with the expected expression pattern.
| SKU | A00736-1 |
|---|---|
| Application | Western Blot |
| Sample | HepG2 subcutaneous xenograft in nude mice |
| Sample Processing Description | ① normal rat colon tissue; ② rat colon cancer model tissue; total protein extracted. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | HuR/ELAVL1 Antibody Picoband® |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 30 min in 37℃ |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | HUR protein is an RNA-binding protein that plays multiple key roles in gene expression regulation. It is essential in maintaining cellular homeostasis, stress response, inflammation, and the development and progression of diseases, especially cancer. In normal tissues, it is expressed at moderate levels, maintaining a delicate balance of cell proliferation, stress response, inflammation, and neural functions, while in tumors it is highly overexpressed, driving malignant progression. The experimental results are consistent with these observations. |
Xilinguli, Xinjiang Medical University
Verified customer
Submitted 2026-04-22
Product Promise
We promise Anti-HuR/ELAVL1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A00736-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026