ELAV-like protein 4 Antibodies

ELAV-like protein 4 (ELAVL4)

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Gene Information

Human
Gene Name:	ELAVL4
Uniprot:	P26378
Entrez:	1996

Family

Belongs to:
RRM elav family

Alternative Names

abnormal vision, Drosophila, homolog of, like-4; ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D); HuD; Paraneoplastic encephalomyelitis antigen HuD

Molecular Weight

Mass (kDA):
42.398 kDA

Genomic Context

Human
Location:	1p33-p32.3
Sequence:	1; NC_000001.11 (50048055..50203772)

Tissue Specificity

Brain.

Subcellular Localizations

Cytoplasm. Perikaryon. Cell projection, dendrite. Cell projection, axon. Cell projection, growth cone. Co-localizes with ribosomal RNA in polysomes.

Diseases

• Malignant Neoplasm Of Lung
• Small Cell Carcinoma Of Lung
• Malignant Neoplasms
• Neoplasms
• Lung Neoplasms
• Nervousness
• Paraneoplastic Syndromes
• Encephalomyelitis
• Paraneoplastic Encephalomyelitis
• Neuroblastoma
• Paraneoplastic Syndromes, Nervous System
• Carcinoma

• Visual Impairment
• Autoimmunity
• Nervous System Disorder
• Sjogren's Syndrome
• Autoimmune Diseases
• Autoimmune Reaction

Pathways

• Translation
• Immune Response
• Localization
• Cell Cycle
• Cell Death
• Pathogenesis
• Regeneration
• Rna Processing
• Transport
• Brain Development
• Regulation Of Gene Expression
• Nuclear Export
• Cell Cycle Arrest

• System Development
• Cell Proliferation
• Nervous System Development
• Cell Growth
• Gene Silencing
• Neurogenesis
• Gluconeogenesis

PTMs

• Phosphorylation

• Methylation

• Myristoylation

Research Areas

• Neuroscience
• Vision

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ELAVL4

Best Uses Of The ELAVL4 Marker From Boster Bio

Boster Bio makes high-affinity ELAVL4 markers. They are reliable and highly cited over the last 25 years. Additionally, they have been validated by immunohistochemistry, Western Blotting, and ELISA. You can therefore be sure that these antibodies will be effective in your research.

Steven Boster

Steven Boster's study of the ELAVL4 marker revealed its utility in many areas of neuroscience. The ELAVL4 protein is a vital component of synaptic signaling and tauopathy. Overexpression in neurons promotes survival and differentiation. However, it is rare for this marker to be expressed in neurons. It was found in neurons in Parkinson's disease and Alzheimers disease.

ELAVL4 Marker

In sporadic cases ALS patients, ELAVL4 protein colocalizes to FUS-positive inclusions. This suggests an aberrant FUS-ELAVL4 crosstalk. Further, mutations in FUS trigger aberrant crosstalk with ELAVL4, suggesting implications in the etiology of ALS. See the Best Uses of the ELAVL4 marker for more information.

Gene Infographics

ELAVL4 RNA-binding protein orthologous with human ELAVL4. It is involved in axonogenesis. It is also found in protein-containing complexes. The gene is expressed extensively in the nervous, particularly in the trigeminal plaque. Gene infographics are a great way to learn about the complexes that your genes are involved in.

Primary antibodies with high-affinity

ELAVL4 is a novel class of RNA-binding protein with high affinity for APP. These proteins are crucial for the skipping exons 7, 8 of APP. The interaction of U2AF65, the upstream 3' Splicing Site, and nELAVLs is inhibited due to the binding of these nELAVLs. SK-N-SH cells were transfected with the ELAVL4 marker. Whole cell lysates collected from the cells were used in RNA pulling down assays. Biotin-labeled, riboprobes were used to extract RNA and induce skipping of exons 7, 8 of APP.

The Elav family is able to recognize HuC, HuD, HelN1 and HuC-N1. This protein codes for a neuronal HIV-1 variant. HuR is present in most proliferating cells and is not detected by this antibody. These antibodies are specific for vertebrate species and can be used to label neuronal cells in zebrafish and chick. The labeling becomes apparent very early in development, and the antibodies are active when neurons have exited the mitotic cycle.

These experiments also confirmed the interaction of mutant FUS with ELAVL4 by co-immunoprecipitation. ELAVL4 co-immunoprecipitated with mutant FUS in double reporter HeLa cells. Extracts treated by RNase A showed no ELAVL4 signals. This study also found that human iPSC derived FUSP525L-expressing MNs have endogenous FUSP525L.

nELAVLs react with APP premRNA. They can be detected by RIP as well as RT-PCR. Using RT-PCR, primers for nELAVLs were amplified from the exon/intron boundaries of exons 7 and 8. Immunoprecipitates from SK-N-SH and SH-SY5Y cells expressing nELAVLs showed a significant amount of APP pre-mRNA, whereas GAPDH pre-mRNA was not present.

These nELAVLs act as RBPs to regulate APP695 transcription. SKN-SH cells were cotransfected with plasmids expressing AUF-1 and ELAVL4 markers. Co-transfection experiments were carried out using the APPE78 human or mouse minigenes and the ELAVL4/p42 AUF1-expression plasmids.