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- Table of Contents
Facts about Flotillin-1.
Human | |
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Gene Name: | FLOT1 |
Uniprot: | O75955 |
Entrez: | 10211 |
Belongs to: |
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band 7/mec-2 family |
flotillin 1; flotillin-1; integral membrane component of caveolae
Mass (kDA):
47.355 kDA
Human | |
---|---|
Location: | 6p21.33 |
Sequence: | 6; NC_000006.12 (30727709..30742851, complement) |
Cell membrane; Peripheral membrane protein. Endosome. Membrane, caveola; Peripheral membrane protein. Melanosome. Membrane raft. Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Membrane-associated protein of caveola (By similarity).
If you are looking for a FLOT1 antibody, you've come to the right place. This article will explain how to optimize the antibody and use them for IHC/ICC validation. Read on to discover the benefits of Boster Bio's FLOT1 antibody and learn how you can apply it in your laboratory. There are several key factors that should be considered before you use it.
Antibodies to flotillin 1 (FLOT1) target the gene and protein of the same name. This protein is a part of caveolae. It is approximately 47 kilobaltons long. Different types of antibodies can be used for different purposes. This article will explain the differences and how to use them. Boster Bio's Anti-Flotillin 1FLOT1 Marker was designed to detect flotillin from biological samples.
This protein is expressed in many other malignant tumors, as well as human disease. The International Radiation Hybrid Mapping Consortium mapped the FLOT1 gene to chromosome 6. Bickel et al. Bickel et.al. found that mouse Flot1 behaves like a resident integral protein in caveolae. They also copurified Flot2 with caveolin-1. Boster Bio can use the Anti-Flotillin 1 FLOT1 Marker in clinical trials to detect this particular protein in cancer cells.
FLOT-1 overexpression in gastric cancer leads to increased cell proliferation and decreased expression of miR-485-5p. This gene targets HCC's oncogene, which promotes metastasis. FLOT-1 levels high in the body can cause poor survival and accelerate cancer progression. It is essential to find the most accurate anti-Flotillin 1/FLOT1 marker to detect this protein-related to cancer.
BosterBio's flow cytometry manual provides useful protocols, optimization tips, troubleshooting points, and other tips for this well-known cell biology technique. Flow Cytometry uses laser-based technology in order to count and profile cells in fluid mixtures. It is also called FACS or fluorescence-activated cell sorting. The company's flowcytometry manual includes a stepby-step guide, product datasheets and sample preparation instructions.
IHC-based biomarkers provide confidence in drug development and can help to identify the most beneficial pathologies. It is vital to validate reagents against antibodies. Data in academic publications or commercial data sheets may not be sufficient to prove their usefulness. Validation is a continuous process, and is essential for the successful use of biomarkers in clinical trials.
TMA preparation is essential for consistent IHC data. It is important to use a single-point staining method, as well multiple-color incubations, to ensure quality and reproducibility. The presence of water can cause antigens to become diluted and may complicate the validation process. TMAs must be prepared with care.
To confirm specificity, antibodies must first be tested on tissue. Evidence of specificity can be provided by Western blot and flow cytometry against panels of positive cell lines. Flow cytometry is a method that can validate antibodies by demonstrating their ability to distinguish nonspecific activities from specific ones. It is also important that a negative control be performed to confirm that the secondary antigen is binding to a certain protein.
The FLOT1 marker is used to validate ICC rules before documents are submitted to SAP. This allows users to perform validation of documents in real-time without having to spend time manually modifying documents. This can be used to identify documents with errors and reject them. A document that fails to validate business rules will display an error message on the screen. If the DP trigger job is completed, the document will be moved to VIM Workflow. Once the job has been completed, the invoice can be sent to AP.
FLOT1 was identified by Li et. al. as a significant factor in the growth of breast cancer cells. It was also found that it inhibits cell proliferation via the miR-122. If FLOT1 is silenced, it prevents the proliferation of MDAMB-231 cells as well as T47D cells. These are both types of breast-cancer cells. Similar results can be achieved with siRNA targeting FLOT1 genes. This inhibits cell invasion and proliferation in MDA/MB-231 cells and T47D Cells.
The FLOT1 gene can be found on the cell membrane. It binds to a specific protein. Immunofluorescence becomes more specific when this protein is bound specifically to a target. The type of virus and cell membrane determine the specificity. FLOT1 is a gene that plays a role in cell membrane production and has been identified as a target by viral RNAi.
Boster Bio FLOT1 marker is used for flow-cytometry validation. It is a high quality, universal antibody that has been thoroughly tested in multiple assays. Studies using different types cells have proven its specificity and sensitivity. The antibody can detect FLOT1 in human, mouse, and rat samples. It reacts with each of these cell types. Flow cytometry data is presented in many graphs including histograms.
This fluorescent dye staining compound is a powerful tool which allows researchers to sort thousands upon thousands of cells in seconds. Flow cytometry can be used to identify cell functions and phenotypes, as it is a quantitative technique. Flow Cytometry can also be used to sort and analyze live cells. It is versatile and the Boster Bio FLOT1 mark is a proven solution.
Flow cytometry uses fluorescence-conjugated antibodies to analyze cell surface antigens. This approach eliminates the problem of non-specific binding that occurs when an antibody-fluorophore complex is large and cannot penetrate the cells. Non-specific binding can also be caused by secondary antibodies that do not penetrate the cells. This is an important step for flow cytometry validation.
PMID: 11167132 by Edgar A.J., et al. Flotillin-1: gene structure: cDNA cloning from human lung and the identification of alternative polyadenylation signals.
PMID: 20682791 by Gorbea C., et al. A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components.
*More publications can be found for each product on its corresponding product page