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- Table of Contents
FLOT1 antibody (PA2033) performs well in IP, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IF analysis of FLOT1 using anti-FLOT1 antibody (PA2033).
FLOT1 was detected in a paraffin-embedded section of human non-small cell lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FLOT1 Antibody (PA2033) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
FLOT1 was detected in a paraffin-embedded section of human non-small cell lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FLOT1 Antibody (PA2033) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Flotillin 1/FLOT1 Antibody Picoband®
FLOT1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IF, IHC, WB |
Tested Applications
FLOT1 antibody was positively detected by WB in:
human A431 whole cell lysates, human U251 whole cell lysates, human PC-3 whole cell lysates, human Hacat whole cell lysates, rat brain tissue lysates, rat skin tissue lysates, mouse brain tissue lysates, mouse skin tissue lysates
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FLOT1 antibody was positively detected by IHC in:
human stomach camcer, human liver cancer tissue, human thyroid cancer tissue, mouse brain, rat brain
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
FLOT1 antibody was positively detected by IP in:
A431 cell
FLOT1 antibody was positively detected by IF in:
human non-small cell lung cancer tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Flotillin 1/FLOT1 Antibody Picoband®
View all FLOT1 Antibodies
View all FLOT1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Flotillin 1, different from the related rat and mouse sequences by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
47 kDa
Calculated Molecular Weight
47.4 kDa
Background of FLOT1
FLOT1 (Flotillin 1), is a protein that in humans is encoded by the FLOT1 gene. The International Radiation Hybrid Mapping Consortium mapped the FLOT1 gene to chromosome 6. Bickel et al. (1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae. It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes. Hazarika et al. (1999) found that stable transfection of Flot1, which they called ESA/flotillin-2, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells. Santamaria et al. (2005) found that prostate tumor overexpressed gene-1 interacted with flotillin-1 in detergent-insoluble membrane fractions. Flotillin-1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase.
Cite This Product
Anti-Flotillin 1/FLOT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2033)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Mouse
94%
High Homology
Rat
94%
High Homology
Zebrafish
69%
Low homology
Drosophila
69%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FLOT1 antibody
Immunohistochemistry (IHC) protocol for FLOT1 antibody
Immunoprecipitation (IP) protocol for FLOT1 antibody
Immunofluorescence (IF) protocol for FLOT1 antibody
Publications
Application:
Species:
Aggregation of lipid rafts activates c-met and c-Src in non-small cell lung cancer cells
Published: 2018-05-30
Journal: BMC CANCER
Application: WB
Impact Factor: 3.29
Species: Human
PMID: 29848294
β-Cyclodextrin induces the differentiation of resident cardiac stem cells to cardiomyocytes through autophagy
Published: 2017-05-15
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
Application: WB
Impact Factor: 4.52
Species: Mouse
PMID: 28522298
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Product Promise
We promise Anti-Flotillin 1/FLOT1 Antibody Picoband® performs excellently in relevant applications for IP, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA2033
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026