Fto Antibodies

AND Fto ELISA kits, Fto Proteins

Many biological assays use antibodies to detect Fto, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Fto in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Fto antibodies...

We validate the specificity of these antibodies to Fto by testing them on tissues known to express Fto positively and negatively. Browse below to find the Fto antibody that suites your experiment. We have 8 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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Fto Infographic by Boster Bio

All Fto Products

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Specificity

Applications

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Anti-FTO Monoclonal Antibody

1 Q&As

Western blot analysis of FTO using anti-FTO antibody (M00219)

Immunofluorescent analysis using the Antibody at 1:50 dilution

Human, Rat

IF, IHC, ICC, WB

$370
Cat # M00219
100 μl

Anti-FTO Antibody

Western blot analysis of FTO in human uterus tissue lysate with FTO antibody at (A) 1 and (B) 2 μg/mL

Immunohistochemistry of FTO in mouse brain tissue with FTO antibody at 2

Immunofluorescence of FTO in Mouse Brain cells with FTO antibody at 20 μg/mL

Human

ELISA, IF, IHC-P, WB

$481
Cat # A00219
0.1 mg

Anti-FTO Antibody

Human

IF, IHC, ICC, WB

$405
Cat # A00219-1
100ul

Anti-FTO Antibody

Human, Mouse, Rat

ELISA, WB

$380
Cat # A00219-2
100ul

Anti-FTO Antibody Picoband®

3 Citations

Western blot analysis of FTO using anti-FTO antibody (A00219-3)

IHC analysis of FTO using anti-FTO antibody (A00219-3)

Human, Mouse, Rat

ELISA, Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A00219-3
100 μg/vial

Anti-FTO Mouse Monoclonal Antibody [Clone ID: OTI4A1]

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FTO mouse monoclonal antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FTO mouse monoclonal antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FTO mouse monoclonal antibody

Human, Mouse, Rat

IHC, WB

$523
Cat # M00219-1
100 µl

FTO (NM_001080432) Human Over-expression Lysate

Human

$960
Cat # LS079068
20 µg, 100 µg

Anti-FTO Rabbit Monoclonal Antibody

Human kidney was stained with anti-FTO rabbit antibody

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti- FTO antibody

Human, Mouse, Rat

ELISA, IP, IF, IHC, WB

$146
Cat # M00219-2
20μL, 40μL, 100μL, 200μL

Fto Overview

Facts about Alpha-ketoglutarate-dependent dioxygenase FTO.

Alpha-ketoglutarate-dependent dioxygenase FTO (Fto)

Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine.

Especially demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Has no activity towards 1- methylguanine.

Gene Information

Mouse
Gene Name:	Fto
Uniprot:	Q8BGW1
Entrez:	26383

Family

Belongs to:

fto family

Alternative Names

Alpha-ketoglutarate-dependent dioxygenase FTO; EC 1.14.11.-; fat mass and obesity associated; Fat mass and obesity-associated protein; FTO; KIAA1752protein fto; MGC5149

Molecular Weight

Mass (kDA):
58.007 kDA

Genomic Context


Mouse

Location:	8 C4-C5\|8 44.34 cM
Sequence:	8;

Tissue Specificity

Ubiquitous. Detected in brain, brain cortex, hypothalamus, cerebellum, liver, pancreas, heart, kidney, white adipose tissue and skeletal muscle. Most abundant in the brain, particularly in hypothalamic nuclei governing energy balance.

Diseases

Obesity
Diabetes Mellitus
Diabetes Mellitus, Non-insulin-dependent
Insulin Resistance
Malignant Neoplasms
Cardiovascular Diseases
Dysequilibrium Syndrome
Pediatric Obesity
Insulin Sensitivity
Hypertensive Disease
Metabolic Syndrome X
Polycystic Ovary Syndrome

Obesity, Morbid
Impaired Glucose Tolerance
Atherosclerosis
Nervousness
Neoplasms
Diabetes Mellitus, Insulin-dependent

Pathways

Pathogenesis
Secretion
Energy Homeostasis
Insulin Secretion
Transport
Methylation
Dna Methylation
Demethylation
Light Absorption
Localization
Glucose Homeostasis
Aging
Eating Behavior

Feeding Behavior
Regeneration
Electron Transport
Photosynthesis
Oxidative Demethylation
Detection Of Glucose
Cell Proliferation

PTMs

Oxidation
Methylation
Demethylation

Phosphorylation
Reduction
Cleavage

Nitration

Research Areas

Lipid and Metabolism
Neuroscience

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Fto

References

PMID: 10501967 by Peters T., et al. Cloning of Fatso (Fto), a novel gene deleted by the Fused toes (Ft) mouse mutation.

PMID: 17991826 by Gerken T., et al. The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase.

1 Publications of Boster Fto Antibodies & ELISA Kits

PubMed ID: 10.1002/cbin.11490, Fat mass and obesity‐associated protein regulates lipogenesis via m6A modifica...

*More publications can be found for each product on its corresponding product page