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- Table of Contents
Fto Antibodies
AND Fto ELISA kits, Fto Proteins
Many biological assays use antibodies to detect Fto, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Fto in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Fto antibodies...
Designing a Western blot for Fto? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the FTO Western Blot GuideDesigning an IHC experiment for Fto? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the FTO IHC GuideFto Infographic by Boster Bio
All Fto Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA, Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A00219-3
100 μg/vial
Cat # A00219-3
100 μg/vial
Human, Mouse, Rat
ELISA, IP, IF, IHC, WB
$146
Cat # M00219-2
20μL, 40μL, 100μL, 200μL
Cat # M00219-2
20μL, 40μL, 100μL, 200μL
Fto Overview
Facts about Alpha-ketoglutarate-dependent dioxygenase FTO.
Alpha-ketoglutarate-dependent dioxygenase FTO (Fto)
Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine.
Especially demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Has no activity towards 1- methylguanine.
Gene Information
| Mouse | |
|---|---|
| Gene Name: | Fto |
| Uniprot: | Q8BGW1 |
| Entrez: | 26383 |
Family
| Belongs to: |
|---|
| fto family |
Alternative Names
Alpha-ketoglutarate-dependent dioxygenase FTO; EC 1.14.11.-; fat mass and obesity associated; Fat mass and obesity-associated protein; FTO; KIAA1752protein fto; MGC5149
Molecular Weight
Mass (kDA):
58.007 kDA
Genomic Context
| Mouse | |
|---|---|
| Location: | 8 C4-C5|8 44.34 cM |
| Sequence: | 8; |
Tissue Specificity
Ubiquitous. Detected in brain, brain cortex, hypothalamus, cerebellum, liver, pancreas, heart, kidney, white adipose tissue and skeletal muscle. Most abundant in the brain, particularly in hypothalamic nuclei governing energy balance.
Diseases
- Obesity
- Diabetes Mellitus
- Diabetes Mellitus, Non-insulin-dependent
- Insulin Resistance
- Malignant Neoplasms
- Cardiovascular Diseases
- Dysequilibrium Syndrome
- Pediatric Obesity
- Insulin Sensitivity
- Hypertensive Disease
- Metabolic Syndrome X
- Polycystic Ovary Syndrome
Pathways
- Pathogenesis
- Secretion
- Energy Homeostasis
- Insulin Secretion
- Transport
- Methylation
- Dna Methylation
- Demethylation
- Light Absorption
- Localization
- Glucose Homeostasis
- Aging
- Eating Behavior
PTMs
- Oxidation
- Methylation
- Demethylation
Research Areas
- Lipid and Metabolism
- Neuroscience
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Fto
bosterbio.com/bosterbio-gene-info-cards/Fto
References
PMID: 10501967 by Peters T., et al. Cloning of Fatso (Fto), a novel gene deleted by the Fused toes (Ft) mouse mutation.
PMID: 17991826 by Gerken T., et al. The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase.
1 Publications of Boster Fto Antibodies & ELISA Kits
- PubMed ID: 10.1002/cbin.11490, Fat mass and obesity‐associated protein regulates lipogenesis via m6A modifica...
*More publications can be found for each product on its corresponding product page