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Product Info Summary
| SKU: | A00219 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-FTO Antibody
SKU/Catalog Number
A00219
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-FTO Antibody (Catalog # A00219). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
FTO antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-FTO Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00219)
Host
Rabbit
Contents
FTO Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FTO antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FTO. The immunogen is located within the first 50 amino acids of FTO.
Reactive Species
A00219 is reactive to FTO in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
58.3 kDa
Background of FTO
Rising obesity rates are rapidly becoming a growing health concern in the developing world. The fat mass and obesity associated gene (FTO) is the first gene discovered to contribute to common forms of human obesity. FTO is a member of the non-heme dioxygenase superfamily, encoding a 2-oxoglutarate-dependent nucleic acid demethylase whose mRNA is widely expressed, especially in neurons of feeding-related nuclei of the brain. FTO mRNA in the arcuate nucleus in mice is up-regulated by feeding and down-regulated during fasting, although the opposite pattern has been observed in rats. At least four isoforms of FTO are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00219 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FTO antibody can be used for detection of FTO by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FTO in human uterus tissue lysate with FTO antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of FTO in mouse brain tissue with FTO antibody at 2.5 μg/mL.
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Immunofluorescence of FTO in Mouse Brain cells with FTO antibody at 20 μg/mL.
Specific Publications For Anti-FTO Antibody (A00219)
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