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- Table of Contents
FTO antibody (A00219) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of FTO in Mouse Brain cells with FTO antibody at 20 μg/mL.
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Anti-FTO Antibody
FTO antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
FTO antibody can be used for detection of FTO by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-FTO Antibody
View all FTO Antibodies
View all FTO Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
FTO antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
FTO Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FTO antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FTO. The immunogen is located within the first 50 amino acids of FTO.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
58.3 kDa
Background of FTO
Rising obesity rates are rapidly becoming a growing health concern in the developing world. The fat mass and obesity associated gene (FTO) is the first gene discovered to contribute to common forms of human obesity. FTO is a member of the non-heme dioxygenase superfamily, encoding a 2-oxoglutarate-dependent nucleic acid demethylase whose mRNA is widely expressed, especially in neurons of feeding-related nuclei of the brain. FTO mRNA in the arcuate nucleus in mice is up-regulated by feeding and down-regulated during fasting, although the opposite pattern has been observed in rats. At least four isoforms of FTO are known to exist.
Cite This Product
Anti-FTO Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00219)
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Product Promise
We promise Anti-FTO Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00219
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026