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- Table of Contents
FTO antibody (M00219) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Anti-FTO Monoclonal Antibody
FTO antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
FTO antibody was positively detected by WB in:
human 293T whole cell, human Hela whole cell, human SiHa whole cell, human Daudi whole cell, human HepG2 whole cell, human T-47D whole cell, human K562 whole cell, rat RH35 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FTO antibody was positively detected by IHC in:
human breast cancer tissue, human lung cancer tissue, human esophagus cancer tissue, human rectal cancer tissue, human ovarian cancer tissue, human liver cancer tissue, human pancreas cancer tissue, human stomach cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
FTO antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-FTO Monoclonal Antibody
View all FTO Antibodies
View all FTO Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEAA-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FTO Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine.
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
58 kDa
Calculated Molecular Weight
58.3 kDa
Cite This Product
Anti-FTO Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00219)
Protocols
Western Blot protocol for FTO antibody
Immunohistochemistry (IHC) protocol for FTO antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for FTO antibody
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Product Promise
We promise Anti-FTO Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00219
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026