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GNAQ / Guanine nucleotide-binding protein G(q) subunit alpha · IHC design guide
Design Immunohistochemistry for GNAQ
Plan GNAQ staining in paraffin sections using colon or prostate glandular cells as reference tissues (HPA tissue IHC). This guide covers fixation consistency, a primary antibody range of 0.5–1 μg/mL (datasheet PB9707), chromogenic detection, and interpretation of uncertain tissue staining (HPA tissue IHC).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
GNAQ Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasmic and membranous staining (HPA tissue IHC) | |
| Staining pattern | Variable cytoplasmic and membranous staining in glandular cells (HPA tissue IHC) | |
| Antigen retrieval | EDTA pH 8.0 HIER, heat-mediated (datasheet PB9707) | |
| Positive control | Appendix+4 more · see all | |
| Negative control | Adipose tissue+4 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image PB9707) | |
| Caveat | Staining is uncertain; the antibody may target more than one gene (HPA tissue IHC) | |
| Regulation | Reduced in rheumatoid arthritis PBLs (UniProt) | |
| Isoform / epitope | No annotated isoforms; one chain spans residues 1–359 (UniProt) |
Section 1
Recommended GNAQ IHC & IF Protocols
The catalog antibody uses EDTA retrieval (datasheet PB9707); the three published GNAQ IHC protocols below cover sheep testis and epididymis, skin, and hen uterovaginal junction (PMC5088417; PMC10392076; PMC8082553).
| Sample | Paraffin-embedded rat ovary tissue; fixative not specified (datasheet PB9707) |
| Fixation | Image fixative and duration unreported (datasheet PB9707); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: EDTA pH 8.0 (datasheet PB9707); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet PB9707) |
| Primary antibody | Rabbit anti-GNAQ, 0.5-1μg/ml (datasheet PB9707) |
| Primary incubation | Overnight at 4 °C (datasheet PB9707) |
| Detection | Streptavidin-biotin complex (SABC), DAB chromogen (datasheet PB9707) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | GNAQ-positive staining in glandular cells of appendix (HPA tissue IHC: Medium). HPA tissue profile: Cytoplasmic and membranous expression at variable levels in several tissues. No signal in the no-primary control. |
💡Decision noteStart with heat-mediated EDTA at pH 8.0 for the catalog antibody (datasheet PB9707). The cited studies used citrate at pH 6.0 where retrieval was reported (PMC10392076; PMC8082553).
Section 2
What Is the Expected GNAQ Staining Pattern?
GNAQ is associated with the plasma membrane through a lipid anchor and has no transmembrane segment; UniProt also lists Golgi, nuclear and nuclear-membrane locations (UniProt P50148 topology and subcellular location). In tissue IHC, expect variable cytoplasmic and membranous staining, including medium staining in colon and prostate glandular cells (HPA tissue IHC). Interpret cautiously: HPA rates tissue IHC Uncertain because antibody staining and RNA data have low consistency.
| Membranous staining with some cytoplasmic signal in colon or prostate glandular cells. | This fits the reported tissue pattern; both cell populations show medium staining (HPA tissue IHC). Compare the two compartments separately when scoring. A plausible pattern supports interpretation, but does not independently establish antibody specificity: HPA rates tissue IHC Uncertain and cautions that antibodies may target protein from more than one gene (HPA tissue IHC). |
| Strong nuclear-only staining, with no convincing membranous or cytoplasmic signal. | Recheck the interpretation before calling this the expected tissue pattern: HPA describes tissue IHC as cytoplasmic and membranous (HPA tissue IHC). Nuclear staining is not automatically an artifact, since UniProt lists nucleus and nuclear membrane (UniProt P50148 subcellular location). Nuclear speckles come from ICC-IF evidence and have uncertain support (HPA subcellular). |
| Prominent staining in cells reported as undetected, such as adipocytes or lung alveolar cells. | Those cell types are reported as Not detected in tissue IHC (HPA tissue IHC). Check the cell identity and compare a control section. Persistent signal could reflect cross-reactivity or, with enzyme-based chromogenic detection, endogenous activity (general IHC practice). The HPA tissue rating is Uncertain, so a mismatch warrants investigation rather than an automatic biological conclusion. |
| Diffuse color covers tissue and blank areas without a clear cellular boundary. | This is difficult to score as GNAQ because the reported pattern is cellular, cytoplasmic and membranous (HPA tissue IHC). Background from detection reagents or nonspecific binding can obscure compartment assignment (general IHC practice). Compare the no-primary control and review blocking, washing and detection before assigning an intensity score. |
| No staining in colon or prostate glandular cells while the section otherwise appears interpretable. | These are useful comparison cells because medium staining was observed in both (HPA tissue IHC). An absent result may reflect assay performance or a different sample result; the HPA tissue assessment is Uncertain (HPA tissue IHC). Review the catalog antibody's IHC-P conditions and controls before calling the tissue GNAQ-negative (general IHC practice). |
💡Expected GNAQ appearanceCall a result provisionally positive when colon or prostate glandular cells show medium cytoplasmic and membranous staining (HPA tissue IHC); diffuse, cell-independent color is suspect background (general IHC practice).
| Tissue IHC evidence | Colon and prostate glandular cells are medium, while adipocytes and lung alveolar cells are Not detected (HPA tissue IHC). These are comparison patterns, not definitive controls: HPA rates the tissue result Uncertain and flags possible recognition of protein from more than one gene (HPA tissue IHC). |
| Membrane association and compartment | A lipid anchor associates GNAQ with the plasma membrane despite the absence of a transmembrane segment (UniProt P50148 topology and subcellular location). UniProt also lists Golgi, nucleus and nuclear membrane; tissue IHC primarily describes cytoplasmic and membranous expression (UniProt P50148 subcellular location; HPA tissue IHC). |
| Expression across tissues | UniProt describes predominant expression in ovary, prostate, testis and colon (UniProt P50148 tissue specificity). HPA reports low tissue RNA specificity and variable tissue IHC staining (HPA tissue IHC). Use the observed cell-level IHC pattern when reading a section, while keeping HPA's Uncertain reliability rating in view. |
| IF/ICC Q&A: should its pattern set the tissue IHC call? | No. Plasma-membrane localization is supported in ICC-IF, whereas nuclear speckles and cytosol are uncertain (HPA subcellular). Tissue IHC reports cytoplasmic and membranous staining and has Uncertain reliability (HPA tissue IHC). Read each application against its own evidence; this section supplies no IF/ICC protocol. |
| Situation | Likely cause | Next action |
|---|---|---|
| Colon and prostate glandular cells have no convincing signal. | The observed medium tissue pattern has not been reproduced (HPA tissue IHC); the cause cannot be assigned from this slide alone. | Check that the catalog antibody's IHC-P conditions were followed, and review the positive control, retrieval, dilution and detection records (general IHC practice). HPA's Uncertain rating limits how strongly one negative section can be interpreted (HPA tissue IHC). |
| The entire section has diffuse chromogen, including areas without identifiable cells. | Detection background or nonspecific binding may be obscuring the reported cellular pattern (general IHC practice; HPA tissue IHC). | Compare a no-primary control; inspect reagent blocking, wash quality and detection exposure (general IHC practice). Score GNAQ only where a cell and its compartment can be identified, using the cytoplasmic and membranous pattern as the comparison (HPA tissue IHC). |
| Lung alveolar cells or adipocytes stain as strongly as the proposed positive cells. | Both cell types are reported as Not detected (HPA tissue IHC). A discrepancy may arise from cell misidentification, cross-reactivity or chromogenic endogenous activity (general IHC practice). | Confirm morphology, inspect the no-primary control and compare a reported medium-staining cell population (general IHC practice; HPA tissue IHC). Treat persistent staining as unresolved because HPA flags possible recognition of protein from more than one gene (HPA tissue IHC). |
| A nuclear-only pattern dominates the section. | It differs from the reported tissue IHC pattern (HPA tissue IHC), although nuclear and nuclear-membrane localization appear in UniProt (UniProt P50148 subcellular location). | Check the counterstain and control section, then record nuclear staining separately from membrane and cytoplasm (general IHC practice). Do not use uncertain ICC-IF nuclear-speckle localization as confirmation of tissue IHC specificity (HPA subcellular). |
| Staining varies between fields or between the selected comparison tissues. | Variable levels across tissues are part of the HPA tissue description (HPA tissue IHC); uneven processing can also affect IHC appearance (general IHC practice). | Assess cell type and compartment in each field, then compare section quality and controls before interpreting the variation (general IHC practice). Document intensity by cell population rather than assigning one score to the whole section. |
Experiment evidence
IHC & IF images showing GNAQ staining
PB9707 · IHCIHC analysis of GNAQ using anti-GNAQ antibody (PB9707). GNAQ was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated
PB9707 · IHCIHC analysis of GNAQ using anti-GNAQ antibody (PB9707). GNAQ was detected in a paraffin-embedded section of mouse testis tissue. Heat mediat
PB9707 · IFIF analysis of GNAQ using anti-GNAQ antibody (PB9707). GNAQ was detected in immunocytochemical section of A431 cells. Enzyme antigen retriev
M00898 · IHCIHC analysis of GNAQ using anti GNAQ antibody (M00898). GNAQ was detected in paraffin-embedded section of human ovarian cancer tissue. Heat
M00898 · IHCIHC analysis of GNAQ using anti GNAQ antibody (M00898). GNAQ was detected in paraffin-embedded section of human ovarian cancer tissue. Heat
M00898-1 · IFImmunofluorescent analysis using the Antibody at 1:50 dilution.
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000156052-GNAQ/tissue/cerebral+cortex#imid_13802729
HPA · StomachHuman Protein Atlas IHC, Stomach. Source: https://www.proteinatlas.org/ENSG00000156052-GNAQ/tissue/stomach+1#imid_16239139
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000156052-GNAQ/tissue/colon#imid_13803729
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000156052-GNAQ/tissue/liver#imid_13803722
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody CAB010036; green = GNAQ, blue = nucleus, red = microtubules.
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody CAB010036; green = GNAQ, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for GNAQ IHC & IF
🧪Run colon first: glandular cells should stain at a Medium level (HPA: colon glandular cells, Medium). Use esophagus squamous epithelium as the negative tissue (HPA: esophagus squamous epithelial cells, Not detected); on the colon slide, non-glandular cells should be assessed as background comparators only if independently confirmed negative, since their GNAQ status is not supplied (HPA: colon row reports glandular cells only).
Positive control tissue: Appendix (Glandular cells, HPA Medium)
Negative control tissue: Adipose tissue (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show GNAQ in A-431, MCF-7, U2OS, with annotated localisation: Nuclear speckles (uncertain), Plasma membrane (supported) (HPA subcellular).
Technical controls: Include no-primary (secondary-only) and host- and immunoglobulin-class-matched isotype controls, alongside a GNAQ knockout sample or validated peptide block as a biological specificity control (standard IHC practice; caption: rabbit primary antibody). Quench endogenous peroxidase and assess endogenous biotin in colon when using the caption’s biotin-based DAB detection (standard IHC practice; caption: biotinylated secondary, SABC and DAB).
⚠️Feasibility: No target-specific fixation window or fixation effect is reported, and the selected PB9707 rat-ovary paraffin-section caption leaves the fixative unreported (caption: PB9707). That caption uses heat retrieval in EDTA at pH 8.0, but does not establish whether retrieval is required for all specimens (caption: PB9707); the supplied evidence does not establish that frozen sections or IF/ICC are easier than paraffin IHC (caption: PB9707; HPA: ICC-IF images in A-431, MCF-7 and U2OS). For colon sections, distinguish true glandular staining from endogenous peroxidase or biotin signal using the controls above (HPA: colon glandular cells, Medium; caption: SABC/DAB; standard IHC practice).
HPA tissue IHC evidence for GNAQ
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Uncertain — Low consistency between antibody staining and RNA expression data. Caution, targets protein from more than one gene. Pending external verification.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | Glandular cells | Medium | Protein (IHC) | HPA → |
| Caudate | Glial cells | Medium | Protein (IHC) | HPA → |
| Cerebellum | Purkinje cells | Medium | Protein (IHC) | HPA → |
| Colon | Glandular cells | Medium | Protein (IHC) | HPA → |
| Endometrium | Cells in endometrial stroma | Low | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | Adipocytes | Not detected | Protein (IHC) | HPA → |
| Bone marrow | Hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Bronchus | Respiratory epithelial cells | Not detected | Protein (IHC) | HPA → |
| Esophagus | Squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
| Heart muscle | Cardiomyocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced GNAQ IHC Tips
Troubleshoot GNAQ staining in paraffin sections by checking retrieval, compartment pattern, controls and cell-specific scoring.
What retrieval should I try first for weak GNAQ staining?
Start with heat-mediated antigen retrieval in EDTA at pH 8.0 for paraffin-section GNAQ IHC (datasheet PB9707). The selected tissue image used this retrieval before incubation with 1 μg/ml primary antibody overnight at 4°C (datasheet PB9707). If staining is weak, check that sections received consistent heating and cooling, then compare retrieval duration on adjacent sections while holding antibody concentration and detection constant (standard IHC practice). Include a positive control section in each run, because a uniformly weak slide can reflect retrieval or detection failure rather than low GNAQ expression (standard IHC practice).
Could fixation explain inconsistent GNAQ staining between paraffin blocks?
GNAQ-specific sensitivity to fixative type or fixation duration is unknown from the supplied evidence; the selected paraffin-section caption does not state its fixative (datasheet PB9707). Record each block’s fixative and processing history, and compare matched sections with the same EDTA pH 8.0 retrieval and primary-antibody incubation (datasheet PB9707; standard IHC practice). If blocks differ, use a control section processed alongside each block to separate specimen variation from staining-run variation (standard IHC practice). Do not attribute a staining difference to a particular fixation effect without a controlled comparison, even when one block shows clearer membrane or cytoplasmic staining (standard IHC practice; HPA tissue IHC).
Which GNAQ staining compartments are plausible in tissue sections?
Assess membrane-associated and cytoplasmic staining first: tissue IHC reports both patterns, and GNAQ associates with the plasma membrane through a lipid anchor (HPA tissue IHC; UniProt P50148). The protein has no transmembrane segment, so a visible membrane rim does not imply that its epitope faces the extracellular space (UniProt P50148). UniProt also lists Golgi and nuclear locations, while the reported nuclear-speckle localisation is uncertain; interpret a predominantly nuclear pattern cautiously (UniProt P50148; HPA subcellular). Compare the same cell population across adjacent fields and controls before calling a change in compartment distribution biological (standard IHC practice).
How should an unknown GNAQ epitope affect antibody validation?
The supplied record lists no GNAQ isoforms and describes one 1–359 chain with a G-alpha domain at residues 38–359 (UniProt P50148). An epitope sequence or position is not supplied for the catalog antibody, so retrieval performance alone cannot establish which part of GNAQ it recognizes (datasheet PB9707; standard IHC practice). Because GNAQ is lipid-anchored and lacks a transmembrane segment, avoid interpreting staining as evidence for an extracellular epitope (UniProt P50148). If an epitope-dependent result matters, obtain epitope documentation or compare an independently characterized antibody on adjacent sections, with matched compartment and cell-type assessment (standard IHC practice).
How can IF help check a disputed GNAQ IHC pattern?
For a separate IF/ICC check, multiplex GNAQ with a marker for the expected cell population, such as glandular cells when examining colon, and inspect whether the signals occur in the same cells (HPA tissue IHC; standard IF practice). Choose a fluorophore in a channel with low tissue autofluorescence, and inspect single-channel images and an unstained control before interpreting overlap (standard IF practice). GNAQ is membrane-associated without a transmembrane segment; for an intracellular epitope, permeabilisation may be needed, but the catalog antibody’s epitope side is unreported (UniProt P50148; datasheet PB9707). Treat nuclear-speckle staining cautiously because that localisation is marked uncertain (HPA subcellular).
What should I check when GNAQ DAB staining is diffuse?
The selected paraffin-section workflow used 10% goat serum, a biotinylated secondary antibody, streptavidin–biotin detection and DAB (datasheet PB9707). When staining is diffuse, compare no-primary and secondary-only controls, check wash consistency, and titrate the primary antibody around the documented 1 μg/ml starting concentration (datasheet PB9707; standard IHC practice). Include a peroxide-blocking step for chromogenic detection and consider endogenous biotin as a possible source of background with this detection system (standard IHC practice). Inspect whether signal follows tissue edges, folds or damaged regions rather than the membrane and cytoplasmic pattern reported in tissue IHC (standard IHC practice; HPA tissue IHC).
How should I score GNAQ staining across tissue sections? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell population and compartment before scoring, because reported GNAQ tissue staining varies by cell type and includes cytoplasmic and membranous patterns (HPA tissue IHC). For glandular cells in colon or prostate, report the percentage of positive cells and an intensity-based H-score, using the same threshold and scoring rules across sections (HPA tissue IHC; standard IHC practice). Normalize positive-cell counts to the total eligible cells; if measuring positive-cell density, report cells per mm² of viable tissue (standard IHC practice). Keep membrane and cytoplasmic scores separate, and exclude folds, necrosis and visibly uneven section edges using prespecified criteria (standard IHC practice).
How do I distinguish convincing GNAQ staining from artefact?
A plausible result follows a reproducible membranous or cytoplasmic pattern in intact cells and persists across comparable sections, consistent with reported tissue staining and GNAQ membrane association (HPA tissue IHC; UniProt P50148; standard IHC practice). Colon glandular cells are a useful reported medium-staining reference, but the HPA tissue profile has uncertain reliability and cannot by itself prove antibody specificity (HPA tissue IHC). Treat edge-enhanced, necrotic or no-primary-control signal as possible section or detection artefact, including endogenous enzyme activity with DAB detection (standard IHC practice). Predominantly nuclear staining needs further confirmation because nuclear-speckle localisation is reported as uncertain (HPA subcellular).
Boster reagents
Best GNAQ / Guanine nucleotide-binding protein G(q) subunit alpha IHC Antibodies
The catalog shows GNAQ IHC in paraffin sections of rat ovary, mouse testis, human ovarian cancer and human prostatic cancer (PB9707 and M00898 IHC captions), plus IF in A431 cells (PB9707 IF caption).
Real IHC data
Anti-GNAQ Antibody ®
Cat # PB9707
Real IHC data
Anti-GNAQ Antibody ® (monoclonal, 13H4)
Cat # M00898
Real IF data
Anti-GNAQ Rabbit Monoclonal Antibody
Cat # M00898-1
PB9707 has IHC images from rat ovary, mouse testis and human prostatic cancer paraffin sections, plus IF in A431 cells (PB9707 image captions); M00898 has IHC images from human ovarian cancer and mouse testis paraffin sections (M00898 IHC captions). M00898-1 lists IHC and IF/ICC applications, but its supplied figure shows IF without identifying a sample (M00898-1 applications and IF caption).
Which to pick: For tissue IHC, PB9707 has a rabbit antibody figure in rat ovary paraffin sections, while M00898 has a mouse monoclonal figure in human ovarian cancer paraffin sections; neither caption reports the fixative (PB9707 and M00898 IHC captions; catalog hosts and clone). For IF/ICC, PB9707 has an A431 cell IF image and a listed 2 μg/ml concentration; M00898-1 has an IF image at 1:50 with no sample identified (PB9707 IF caption and datasheet; M00898-1 IF caption). For cross-species work, both PB9707 and M00898 list human, mouse and rat reactivity, while only M00898 also lists monkey reactivity (catalog reactivity).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry P50148 (GNAQ_HUMAN, Guanine nucleotide-binding protein G(q) subunit alpha).
- Human Protein Atlas. GNAQ tissue IHC expression (reliability: Uncertain).
- Human Protein Atlas. GNAQ subcellular location (ICC-IF): Mainly localized to the nuclear speckles and plasma membrane. In addition localized to the cytosol..
- Human Protein Atlas. GNAQ antibody validation summary (2 antibodies).
- Molecular Cloning, mRNA Expression, and Localization of the G-protein Subunit Galphaq in Sheep Testis and Epididymis. Asian-Australasian journal of animal sciences 2016 — PMC5088417.
- Transcriptome Profiling of Anhidrotic Eccrine Sweat Glands Reveals that Olfactory Receptors on Eccrine Sweat Glands Regulate Perspiration in a Ligand-Dependent Manner. JID innovations : skin science from molecules to population health 2023 — PMC10392076.
- Transcriptomic and metabolomic insights into the variety of sperm storage in oviduct of egg layers. Poultry science 2021 — PMC8082553.
- GNAQ T96S mutation abrogates the ability of wild-type GNAQ to induce apoptosis by phosphorylating annexin A2 in natural killer/T cell lymphoma. Cancer science 2022 — PMC9277252.
- PubMed PMID:8825633 — UniProt-cited evidence.
- PubMed PMID:8664309 — UniProt-cited evidence.
- PubMed PMID:8836152 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for GNAQ (Guanine nucleotide-binding protein G(q) subunit alpha). Expected localisation Cytoplasmic and membranous staining (HPA tissue IHC). Antigen retrieval: EDTA pH 8.0 HIER, heat-mediated (datasheet PB9707). Validated IHC antibody: PB9707 at 0.5-1μg/ml (the product's own IHC validation image is shown). Controls (HPA): positive Appendix (Glandular cells); negative Adipose tissue. IHC workflow; specimen preparation unspecified. Protocol steps: 1. Sample: Paraffin-embedded rat ovary tissue; fixative not specified (datasheet PB9707); 2. Fixation: Image fixative and duration unreported (datasheet PB9707); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: EDTA pH 8.0 (datasheet PB9707); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet PB9707); 8. Primary antibody: Rabbit anti-GNAQ, 0.5-1μg/ml (datasheet PB9707); 9. Primary incubation: Overnight at 4 °C (datasheet PB9707). Watch for: Fixation: Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image PB9707); Caveat: Staining is uncertain; the antibody may target more than one gene (HPA tissue IHC); Isoform / epitope: No annotated isoforms; one chain spans residues 1–359 (UniProt); Regulation: Reduced in rheumatoid arthritis PBLs (UniProt). Evidence: UniProt P50148, HPA tissue IHC · Uncertain, HPA ICC-IF · Mainly localized to the nuclear speckles and plasma membrane. In…, Datasheet PB9707, PMC5088417, PMC10392076. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/GNAQ/immunohistochemistry-ihc-guide.