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HINT1 / Adenosine 5'-monophosphoramidase HINT1 · IHC design guide
Design Immunohistochemistry for HINT1
Plan chromogenic HINT1 staining in paraffin sections with the catalog antibody’s 0.5–1 μg/mL IHC range (datasheet PB9632). Use cytoplasmic and occasional nuclear staining as the tissue pattern, with kidney tubule cells as a high-staining reference (HPA tissue IHC).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
HINT1 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Mainly cytoplasmic; occasional nuclear staining (HPA tissue IHC) | |
| Staining pattern | Cytoplasmic, sometimes nuclear, in glandular and tubular cells (HPA tissue IHC) | |
| Antigen retrieval | EDTA pH 8.0 HIER, heat-mediated (datasheet PB9632) | |
| Positive control | Epididymis+4 more · see all | |
| Negative control | Heart muscle+4 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image PB9632) | |
| Caveat | Antibody staining and RNA show medium consistency (HPA tissue IHC) | |
| Regulation | Intensity regulation unreported (UniProt) | |
| Isoform / epitope | No listed isoforms; one chain spans residues 2–126 (UniProt) |
Section 1
Recommended HINT1 IHC & IF Protocols
The catalog antibody’s IHC-P protocol is accompanied by published HINT1 staining methods from four articles (PMC3522093; PMC7922225; PMC7592009; PMC11850129).
| Sample | Paraffin-embedded rat kidney tissue; fixative not specified (datasheet PB9632) |
| Fixation | Image fixative and duration unreported (datasheet PB9632); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: EDTA pH 8.0 (datasheet PB9632); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet PB9632) |
| Primary antibody | Rabbit anti-HINT1, 0.5-1μg/ml (datasheet PB9632) |
| Primary incubation | Overnight at 4 °C (datasheet PB9632) |
| Detection | Streptavidin-biotin complex (SABC), DAB chromogen (datasheet PB9632) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | HINT1-positive staining in glandular cells of epididymis (HPA tissue IHC: High). HPA tissue profile: General cytoplasmic and occasional nuclear expression. No signal in the no-primary control. |
💡Decision noteStart with heat-mediated EDTA retrieval at pH 8.0 (PB9632 datasheet); citrate at pH 6.0 is a published alternative (PMC3522093 methods).
Section 2
What Is the Expected HINT1 Staining Pattern?
HINT1 staining in paraffin sections is generally cytoplasmic, with occasional nuclear signal (HPA tissue IHC; UniProt P49773: cytoplasm and nucleus). Look for staining in glandular cells of the epididymis, gallbladder, and seminal vesicle; kidney tubular cells and ovarian follicle cells are also strong reference populations (HPA: High). HINT1 has no transmembrane segment (UniProt P49773 topology). HPA rates the tissue IHC pattern Approved, with medium consistency against RNA expression (HPA tissue IHC).
| Cytoplasmic staining in kidney tubular cells, with occasional stained nuclei. | This fits the reported compartment pattern (HPA tissue IHC) and a High kidney reference population (HPA: High in tubular cells). Score nuclear and cytoplasmic signal separately; occasional nuclear staining can be genuine (UniProt P49773: nucleus; HPA tissue IHC). |
| Signal is concentrated in luminal material or extracellular deposits, with little staining inside the expected cells. | That distribution does not match general cytoplasmic and occasional nuclear HINT1 staining (HPA tissue IHC). Inspect morphology and compare with the detection-only control before assigning it to HINT1 (standard IHC practice). Plasma-membrane localization remains uncertain in ICC-IF (HPA subcellular). |
| Strong staining appears in cardiomyocytes or alveolar cells, while expected positive cell populations are weak. | These populations were Not detected in the supplied tissue survey (HPA: cardiomyocytes; alveolar cells). Recheck cell identification and assay controls; cross-reactivity or endogenous chromogenic activity is possible (standard IHC practice). An HPA Not detected call is not proof of universal absence. |
| Color is diffuse across tissue, empty spaces, or several cell populations without a clear intracellular pattern. | Diffuse color cannot establish the expected cytoplasmic localization (HPA tissue IHC). Check detection-only and substrate controls, washing, and blocking to distinguish background from cellular staining (standard IHC practice). Interpret intensity only after identifying the stained cells. |
| No staining is visible in a section containing kidney tubular cells or gallbladder glandular cells. | Both are High reference populations in the HPA survey (HPA: kidney tubules; gallbladder glandular cells). Confirm that those cells are present and the positive control develops, then review the IHC-validated antibody and detection workflow (standard IHC practice). A single failed section does not establish HINT1 absence. |
💡Expected HINT1 appearanceCall a result positive when identifiable kidney tubular, ovarian follicle, or listed glandular cells show clear cytoplasmic staining, potentially with occasional nuclear signal (HPA: High in these cells; HPA tissue IHC); diffuse deposits or color outside identifiable cells should trigger a background check (standard IHC practice).
| Cell population and tissue context | HPA reports High staining in kidney tubules, ovarian follicle cells, and several glandular populations, but Not detected in cardiomyocytes and alveolar cells (HPA tissue IHC). Compare matched cell populations when judging a section; broad expression alone does not predict equal staining in every cell (UniProt P49773: widely expressed; HPA tissue IHC). |
| Nuclear versus cytoplasmic distribution | Cytoplasm and nucleus are annotated locations, and interaction with CDK7 can shift HINT1 toward the nucleus (UniProt P49773). HPA tissue IHC describes general cytoplasmic and occasional nuclear expression. Record the compartments separately instead of treating every stained nucleus as nonspecific. |
| Membrane interpretation | HINT1 has no transmembrane segment (UniProt P49773 topology). HPA ICC-IF supports cytosol and nucleoplasm, while its plasma-membrane assignment is uncertain (HPA subcellular). An apparently membrane-dominant IHC pattern therefore needs control review before being called a characteristic HINT1 pattern. |
| Evidence and assay limits | The tissue pattern is Approved, with medium consistency against RNA expression (HPA tissue IHC). Antibody HPA044577 is Approved for IHC and Supported for ICC (HPA antibodies). These ratings support cautious interpretation; they do not establish fixation sensitivity, an optimal retrieval condition, or a universal positive threshold. |
| Situation | Likely cause | Next action |
|---|---|---|
| A known High reference population is blank (HPA: kidney tubular cells or gallbladder glandular cells). | The section may lack the identified target cells, or the staining run may have failed (standard IHC practice). | Verify morphology and a run control; review antibody application, retrieval, detection, and counterstain records without assuming a HINT1-specific retrieval requirement (standard IHC practice). |
| The whole section has weak, even chromogenic color. | Endogenous detection activity, incomplete blocking, or residual detection reagent can produce background (standard IHC practice). | Compare detection-only and substrate controls, then review blocking and washing steps (standard IHC practice). Score HINT1 only where intracellular color resolves in identified cells (HPA tissue IHC). |
| Cardiomyocytes or alveolar cells stain strongly (HPA: Not detected in these cells). | Misidentified cells, cross-reactivity, or endogenous chromogenic activity are possible explanations (standard IHC practice). | Check cell morphology and controls, then compare an HPA High reference population in the same staining run (HPA tissue IHC; standard IHC practice). Do not infer specificity from intensity alone. |
| Staining appears exclusively extracellular or in luminal contents. | That pattern differs from general cytoplasmic and occasional nuclear HINT1 staining (HPA tissue IHC). | Inspect whether stain follows cells, compare detection-only controls, and report the discordant distribution rather than scoring deposits as positive cells (standard IHC practice). |
| Nuclear staining looks stronger than expected. | Nuclear localization is possible, and CDK7 interaction can increase it (UniProt P49773); HPA tissue IHC describes nuclear staining as occasional. | Score nuclear and cytoplasmic compartments independently, confirm cell identity, and compare with the cytoplasmic pattern in an HPA High population (HPA tissue IHC; standard IHC practice). |
| Q: Should IF/ICC show the same compartments as tissue IHC? | The assays have different observations: HPA tissue IHC reports general cytoplasmic and occasional nuclear staining; ICC-IF supports cytosol and nucleoplasm, with plasma membrane uncertain (HPA tissue IHC; HPA subcellular). | A: Compare compartment calls within each assay and treat an apparent membrane signal cautiously (HPA subcellular). Consult the separate IF/ICC guide for its workflow; this section interprets paraffin-section IHC. |
Experiment evidence
IHC & IF images showing HINT1 staining
PB9632 · IHCIHC analysis of HINT1 using anti-HINT1 antibody (PB9632). HINT1 was detected in a paraffin-embedded section of rat kidney tissue. Heat media
PB9632 · IHCIHC analysis of HINT1 using anti-HINT1 antibody (PB9632). HINT1 was detected in a paraffin-embedded section of human intestinal cancer tissu
PB9632 · IFIF analysis of HINT1 using anti-HINT1 antibody (PB9632). HINT1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retr
M02557 · IHCM02557 staining HINT1 in human kidney sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue w
M02557 · IFImmunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma cell line) cells la
A02557 · IFImmunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 μg/mL.
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000169567-HINT1/tissue/cerebral+cortex#imid_11949785
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000169567-HINT1/tissue/colon#imid_11907833
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000169567-HINT1/tissue/liver#imid_11907842
HPA · PancreasHuman Protein Atlas IHC, Pancreas. Source: https://www.proteinatlas.org/ENSG00000169567-HINT1/tissue/pancreas#imid_11907793
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA044577; green = HINT1, blue = nucleus, red = microtubules.
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA044577; green = HINT1, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for HINT1 IHC & IF
🧪Run kidney first: cells in tubules should stain strongly (HPA: High in kidney tubules). Use heart muscle cardiomyocytes as the negative tissue (HPA: Not detected in cardiomyocytes); on the kidney slide, cells without specific labeling should show counterstain without DAB deposit, but the supplied HPA rows do not identify a validated HINT1-negative kidney cell type (HPA: High in kidney tubules).
Positive control tissue: Epididymis (Glandular cells, HPA High)
Negative control tissue: Heart muscle (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show HINT1 in A-431, U-251MG, U2OS, KOLF2.1J, with annotated localisation: Plasma membrane (uncertain), Cytosol (supported) (HPA subcellular).
Technical controls: Include a no-primary, secondary-only control, a concentration-matched rabbit IgG isotype control, and HINT1 knockout tissue as a biological negative (caption: rabbit anti-HINT1; standard IHC practice). Block endogenous peroxidase and check for endogenous biotin in kidney when using the caption’s biotin-based SABC/DAB detection (caption: rat kidney, SABC/DAB; standard IHC practice).
⚠️Feasibility: The selected paraffin-section caption reports heat-mediated retrieval in EDTA at pH 8.0, but does not establish whether retrieval is required across other conditions (caption: EDTA retrieval, pH 8.0). Its fixative is unreported, and no target-specific fixation window or fixation effect is supplied (caption: fixative not stated). Paraffin IHC has a documented starting condition here; the supplied evidence does not establish that frozen sections or IF are easier, while kidney endogenous biotin may complicate the caption’s biotin-based detection (caption: rat kidney, SABC/DAB; standard IHC practice).
HPA tissue IHC evidence for HINT1
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Approved — Medium consistency between antibody staining and RNA expression data.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Epididymis | Glandular cells | High | Protein (IHC) | HPA → |
| Fallopian tube | Ciliated cells (cell body) | High | Protein (IHC) | HPA → |
| Gallbladder | Glandular cells | High | Protein (IHC) | HPA → |
| Kidney | Cells in tubules | High | Protein (IHC) | HPA → |
| Ovary | Follicle cells | High | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Heart muscle | Cardiomyocytes | Not detected | Protein (IHC) | HPA → |
| Lung | Alveolar cells | Not detected | Protein (IHC) | HPA → |
| Placenta | Trophoblastic cells | Not detected | Protein (IHC) | HPA → |
| Skeletal muscle | Myocytes | Not detected | Protein (IHC) | HPA → |
| Smooth muscle | Smooth muscle cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced HINT1 IHC Tips
Use the selected paraffin-section image as the starting point for HINT1 IHC, then assess staining by cell type and compartment (datasheet PB9632; HPA tissue IHC).
How should I retrieve HINT1 in paraffin sections when staining is weak?
Start with heat-mediated retrieval in EDTA at pH 8.0 for paraffin sections (datasheet PB9632). The selected image used this retrieval before overnight incubation with 1 μg/ml primary antibody at 4°C (datasheet PB9632). If staining is weak, compare a citrate-buffer retrieval condition on adjacent sections while holding antibody concentration, detection and development constant (standard IHC practice). Include a kidney section with tubules as a positive reference, and check whether stronger staining preserves the expected cytoplasmic and occasional nuclear pattern rather than increasing diffuse background (HPA tissue IHC; standard IHC practice).
Could fixation explain weak or inconsistent HINT1 staining?
The selected tissue image identifies a paraffin-embedded rat kidney section but does not state its fixative (datasheet PB9632). Target-specific sensitivity to fixation is therefore unknown; do not assign a HINT1-specific fixation effect from staining patterns or protein features (datasheet PB9632). For a controlled comparison, process matched specimens with recorded fixation conditions and keep section thickness, EDTA retrieval at pH 8.0, and detection constant (datasheet PB9632; standard IHC practice). Compare tubule staining with tissue preservation and background on the same slide before changing the primary antibody concentration, because damaged morphology can make apparent changes in staining difficult to interpret (HPA tissue IHC; standard IHC practice).
Should HINT1 stain nuclei, cytoplasm, or cell membranes in tissue?
Expect general cytoplasmic and occasional nuclear staining in tissue sections (HPA tissue IHC). UniProt places HINT1 in the cytoplasm and nucleus and notes that interaction with CDK7 can increase nuclear localisation (UniProt P49773). Cell-based imaging supports cytosol and nucleoplasm but lists plasma membrane localisation as uncertain; HINT1 has no transmembrane segment (HPA subcellular; UniProt P49773 topology). Score nuclear and cytoplasmic staining separately in morphologically intact cells, and investigate a membrane-only rim before calling it HINT1, particularly if it follows section edges or appears across unrelated cell types (HPA tissue IHC; standard IHC practice).
Could an isoform or modified epitope explain patchy HINT1 staining?
The supplied record lists 0 isoforms and a HIT domain spanning residues 18–126 (UniProt P49773). It also records acetylated residues and phosphoserines at positions 45 and 72, but supplies no antibody epitope or evidence that these modifications alter IHC binding (UniProt P49773). Treat patchiness first as a question of tissue preservation, retrieval, antibody access or cell composition, assessed on adjacent sections under matched detection conditions (standard IHC practice). If the antibody epitope becomes available, check its position against the annotated residues; do not infer modification-specific staining or an unseen isoform from a spatial pattern alone (UniProt P49773; standard IHC practice).
How can IF help check which cells contain HINT1?
Use IF as a separate validation experiment and multiplex HINT1 with a validated marker for the cell type being examined, such as kidney tubule cells (HPA tissue IHC; standard IF practice). Choose spectrally separated fluorophores, favouring red or far-red detection where tissue autofluorescence obscures shorter wavelengths, and inspect single-channel controls before interpreting overlap (standard IF practice). Optimise permeabilisation to expose cytosolic and nucleoplasmic epitopes, since these are supported locations and HINT1 lacks a transmembrane segment (HPA subcellular; UniProt P49773 topology; standard IF practice). The selected paraffin-section caption supplies an IHC workflow, not an IF fixation or permeabilisation condition, so establish those conditions directly for the IF specimen (datasheet PB9632; standard IF practice).
What should I change when DAB background masks HINT1 staining?
First compare a no-primary control with the stained section to locate background from the secondary reagent or detection system (standard IHC practice). The selected workflow used 10% goat serum blocking, biotinylated goat anti-rabbit secondary antibody, an avidin–biotin complex and DAB (datasheet PB9632). Check endogenous peroxidase blocking and, for this biotin-based workflow, assess endogenous biotin before attributing diffuse DAB deposits to HINT1 (datasheet PB9632; standard IHC practice). Then shorten chromogen development or titrate the primary antibody around the tested 1 μg/ml, retaining a kidney tubule reference to distinguish reduced background from lost specific staining (datasheet PB9632; HPA tissue IHC; standard IHC practice).
How should I quantify HINT1 staining across tissue sections? ⚠ ANSWER MARKED FOR VERIFICATION
Define the scored cell population before counting; HPA reports high staining in kidney tubule cells but no detection in lung alveolar cells (HPA tissue IHC). For a consistent cell population, report the percentage of positive cells and an intensity-based H-score, with nuclear and cytoplasmic results recorded separately (HPA tissue IHC; standard IHC practice). If counting discrete positive cells instead, report density per mm² of evaluable tissue and exclude necrosis, folds and section edges by a prespecified rule (standard IHC practice). Normalise comparisons to the same cell type and sampled area, with matched retrieval, DAB development and counterstaining across slides (standard IHC practice).
How do I distinguish true HINT1 staining from tissue artefact?
A credible result follows the expected cell population and compartment: HPA reports high staining in kidney tubule cells and general cytoplasmic with occasional nuclear expression (HPA tissue IHC). Uniform staining at section edges, in necrotic tissue or across unrelated structures warrants review for processing or detection artefact (standard IHC practice). A membrane-only pattern needs extra scrutiny because plasma membrane localisation is uncertain in cell imaging and the protein has no transmembrane segment (HPA subcellular; UniProt P49773 topology). Compare morphology, a no-primary control and peroxidase-blocked detection before interpreting DAB deposits as HINT1, especially when the pattern conflicts with the reference compartment (standard IHC practice; HPA tissue IHC).
Boster reagents
Best HINT1 / Adenosine 5'-monophosphoramidase HINT1 IHC Antibodies
IHC images show HINT1 in rat and human kidney paraffin sections (PB9632 and M02557 captions); an ICC image shows Jurkat cells (A02557 caption). All three list human, mouse and rat reactivity (catalog reactivity).
Real IHC data
Anti-HINT1 Antibody ®
Cat # PB9632
Real IHC data
Anti-HINT1 Antibody
Cat # M02557
Real IF data
Anti-HINT1 Antibody
Cat # A02557
PB9632 has IHC images of rat kidney, human intestinal cancer and mouse intestine paraffin sections, plus an IF image of A431 cells (PB9632 captions); M02557 has human kidney IHC-P and U-2 OS cell IF images (M02557 captions). A02557 has an ICC image of Jurkat cells and lists ICC among its applications (A02557 caption; catalog applications).
Which to pick: For tissue IHC across the three pictured species, choose PB9632: its IHC captions show rat kidney, human intestinal cancer and mouse intestine paraffin sections, and list 1 μg/ml primary antibody; the fixative is unreported (PB9632 IHC captions). For paraffin-section human kidney IHC-P, M02557 is a mouse monoclonal shown with citrate pH 6 retrieval at 1:25 (M02557 catalog clone and IHC caption). For cell IF, M02557 has a U-2 OS IF image at 1:25, while A02557 has a Jurkat ICC image at 2.5 μg/mL; choose according to the pictured assay (M02557 IF caption; A02557 ICC caption). The selected PB9632 tissue-IHC caption documents paraffin sections, but does not specify the fixative (selected-SKU IHC image PB9632).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry P49773 (HINT1_HUMAN, Adenosine 5'-monophosphoramidase HINT1).
- Human Protein Atlas. HINT1 tissue IHC expression (reliability: Approved).
- Human Protein Atlas. HINT1 subcellular location (ICC-IF): Mainly localized to the plasma membrane and cytosol. In addition localized to the nucleoplasm..
- Human Protein Atlas. HINT1 antibody validation summary (1 antibodies).
- Silencing of Hint1, a novel tumor suppressor gene, by promoter hypermethylation in hepatocellular carcinoma. Cancer letters 2009 — PMC3522093.
- Comparative Proteomic Study Shows the Expression of Hint-1 in Pituitary Adenomas. Diagnostics (Basel, Switzerland) 2021 — PMC7922225.
- Genetic Fine Mapping and Genomic Annotation Defines Causal Mechanisms at A Novel Colorectal Cancer Susceptibility Locus in Han Chinese. Journal of Cancer 2020 — PMC7592009.
- HINT1 suppression protects against age-related cardiac dysfunction by enhancing mitochondrial biogenesis. Molecular metabolism 2025 — PMC11850129.
- PubMed PMID:8812426 — UniProt-cited evidence.
- PubMed PMID:7644499 — UniProt-cited evidence.
- PubMed PMID:14702039 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for HINT1 (Adenosine 5'-monophosphoramidase HINT1). Expected localisation Mainly cytoplasmic; occasional nuclear staining (HPA tissue IHC). Antigen retrieval: EDTA pH 8.0 HIER, heat-mediated (datasheet PB9632). Validated IHC antibody: PB9632 at 0.5-1μg/ml (the product's own IHC validation image is shown). Controls (HPA): positive Epididymis (Glandular cells); negative Heart muscle. IHC-P workflow on paraffin sections; fixative unspecified. Protocol steps: 1. Sample: Paraffin-embedded rat kidney tissue; fixative not specified (datasheet PB9632); 2. Fixation: Image fixative and duration unreported (datasheet PB9632); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: EDTA pH 8.0 (datasheet PB9632); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet PB9632); 8. Primary antibody: Rabbit anti-HINT1, 0.5-1μg/ml (datasheet PB9632); 9. Primary incubation: Overnight at 4 °C (datasheet PB9632). Watch for: Fixation: Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image PB9632); Caveat: Antibody staining and RNA show medium consistency (HPA tissue IHC); Isoform / epitope: No listed isoforms; one chain spans residues 2–126 (UniProt); Regulation: Intensity regulation unreported (UniProt). Evidence: UniProt P49773, HPA tissue IHC · Approved, HPA ICC-IF · Mainly localized to the plasma membrane and cytosol. In addition…, Datasheet PB9632, PMC3522093, PMC7922225. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/HINT1/immunohistochemistry-ihc-guide.