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- Table of Contents
Facts about Stress-70 protein, mitochondrial.
Regulates erythropoiesis through insertion of ISC assembly (PubMed:21123823, PubMed:26702583). May play a role in the control of cell proliferation and cellular aging (By similarity).
Human | |
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Gene Name: | HSPA9 |
Uniprot: | P38646 |
Entrez: | 3313 |
Belongs to: |
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heat shock protein 70 family |
75 kDa glucose-regulated protein; CSA; GRP75; GRP-75; GRP75mthsp75; Heat shock 70 kDa protein 9; heat shock 70kD protein 9B; heat shock 70kDa protein 9 (mortalin); heat shock 70kDa protein 9B (mortalin-2); HSPA9; HSPA9B; HSPA9BMGC4500; Mortalin; mortalin, perinuclear; Mortalin-2; MOT; MOT2; mot-2; MTHSP75; p66-mortalin; PBP74; PBP74CSA; Peptide-binding protein 74; stress-70 protein, mitochondrial
Mass (kDA):
73.68 kDA
Human | |
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Location: | 5q31.2 |
Sequence: | 5; NC_000005.10 (138553756..138575401, complement) |
Mitochondrion. Nucleus, nucleolus.
Boster Bio offers a unique product for the use of scientists. Its antibody to the HSPA9 molecule is designed to detect a particular protein on cells, such as Glycophorin A or CD235a. Scientists can submit their findings for species or application studies to gain product credits. This product is suitable for all scientists, worldwide. This article details the benefits of using Boster Bio's products.
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The high-quality lysate is composed of a mixture of proteins, lipids, carbohydrates, and nucleic acids. Non-specific binding of these components can negatively impact the detection of immunoprecipitated targets. To eliminate non-specific binding, preclearing should be performed on the lysate sample. Magnetic beads seldom require preclearing.
The Protein A/G support binds the heavy chain of the antibody Fc region. The Protein G and L light chains bind the two Fab regions. The Protein A/G supports are compatible with IP experiments but may not be appropriate for some applications. The serum also contains non-specific immunoglobulins that interfere with IP experiments.
Using the high-performance Protein A/G beads ensures the highest quality lysate and provides the best balance between capacity, reproducibility, and purity. Magnetic beads can also be used in 96-well microplates. The high-power magnets localize the magnetic beads so that they are out of the way when pipetting solutions from the beads.
Using high-quality lysate with protein-A/G affinity beads, Boster Bio's antibody A/G reagent is a highly effective, reliable method to purify and quantify HSPA9. These proprietary reagents are suitable for the detection and analysis of human cell-free samples. If you are looking for a high-quality lysate for HSPA9 research, then we recommend using Boster Bio's high-quality lysate with protein A/G affinity beads for HSPA9.
To use protein-A/G magnetic beads, prepare a lysate that contains 70-100 mL of cell-free lysate. Add the beads to the lysate and allow to incubate for 10-30 min at 4degC. Then, wash the beads in lysis buffer and collect the supernatants.
The high-quality lysate with protein-A/G affinity beads from Boster Bio were used to analyze the RNA-bound HSPA9 in cell-free samples. These beads are suitable for use in Western blotting. The high-quality lysate with protein A/G affinity beads for HSPA9 was prepared using Santa Cruz Biotechnology cat. no. sc-715.
PMID: 7684501 by Domanico S.Z., et al. Cloning of the gene encoding peptide-binding protein 74 shows that it is a new member of the heat shock protein 70 family.
PMID: 7829505 by Bhattacharyya T., et al. Cloning and subcellular localization of human mitochondrial hsp70.