Rabbit IgG polyclonal antibody for Stress-70 protein, mitochondrial(HSPA9) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Anti-Grp75/HSPA9 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Applications:||IHC-P, IHC-F, ICC, WB|
|Product Name||Anti-Grp75/HSPA9 Antibody
See all HSPA9 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for GRP75/HSPA9 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. GRP75/HSPA9 information: Molecular Weight: 73680 MW; Subcellular Localization: Mitochondrion . Nucleus, nucleolus .|
|Cite This Product||Anti-Grp75/HSPA9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1789)|
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|Anti-Grp75/HSPA9 Antibody may replace the following items: sc 1058|sc 133137|sc 13967|sc 31650|sc 31652.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Grp75(591-609aa DTETKMEEFKDQLPADECN), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Protein Target Info (Source: Uniprot.org)
|Protein Name||Stress-70 protein, mitochondrial|
|Subcellular Localization||Mitochondrion . Nucleus, nucleolus .|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. .|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Our Boster Quality Guarantee for Anti-Grp75/HSPA9 Antibody covers its use in the following applications.Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti Grp75 (PA1789) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: 293T Whole Cell Lysate at 40ug
Lane 4: M431 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 74KD
Observed bind size: 74KD
IHC(P): Human Lung Cancer Tissue
IHC(P): Rat Liver Tissue
ICC: A549 Cell
Grp75 was detected in frozen section of rat liver tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1Î¼g/ml rabbit anti-Grp75 Antibody (PA1789) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Note: sample size takes longer to process, average 5-7 days.
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