Click image to see more details
-
-
-
-
-
+9
Product Info Summary
| SKU: | M02561-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Grp75 (HSPA9) Mouse Monoclonal Antibody [Clone ID: OTI9F8]
SKU/Catalog Number
M02561-1
Size
100 µl
Description
Boster Bio HSPA9 mouse monoclonal antibody, clone OTI9F8 (formerly 9F8). Catalog# M02561-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Grp75 (HSPA9) Mouse Monoclonal Antibody [Clone ID: OTI9F8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02561-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI9F8
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human HSPA9 (NP_004125) produced in HEK293T cell.
Reactive Species
M02561-1 is reactive to HSPA9 in Dog, Human, Monkey, Mouse, Rat
Observed Molecular Weight
74 kDa
Calculated molecular weight
73.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02561-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000~2000
IHC: 1:50
IF: 1:50
Flow cytometry: 1:100
Validation Images & Assay Conditions
Click image to see more details
Flow cytometric Analysis of Hela cells
Click image to see more details
Flow cytometric Analysis of Jurkat cells
Click image to see more details
Anti-HSPA9 mouse monoclonal antibody (M02561-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA9.
Click image to see more details
Immunofluorescent staining of HepG2 cells using anti-HSPA9 mouse monoclonal antibody (M02561-1).
Click image to see more details
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA9.
Click image to see more details
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-HSPA9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Click image to see more details
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-HSPA9 monoclonal antibody (1:200).
Specific Publications For Anti-Grp75 (HSPA9) Mouse Monoclonal Antibody [Clone ID: OTI9F8] (M02561-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Grp75 (HSPA9) Mouse Monoclonal Antibody [Clone ID: OTI9F8]?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Grp75 (HSPA9) Mouse Monoclonal Antibody [Clone ID: OTI9F8]
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
