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HSPB8 / Heat shock protein beta-8 · IHC design guide
Design Immunohistochemistry for HSPB8
Plan HSPB8 chromogenic IHC around cytoplasmic staining reported across several tissues (HPA tissue IHC). Use pancreatic exocrine cells as a positive reference and adipocytes as a negative reference (HPA tissue IHC); the catalog antibody A02492-2 is listed for IHC at 0.5–1 μg/mL (datasheet A02492-2).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
HSPB8 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasmic staining in several tissues (HPA tissue IHC) | |
| Staining pattern | Cytoplasmic staining across several tissue cell types (HPA tissue IHC) | |
| Antigen retrieval | Citrate pH 6 HIER, heat-mediated (datasheet A02492-2) | |
| Positive control | Cerebral cortex+4 more · see all | |
| Negative control | Adipose tissue+3 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Keep tissue fixation consistent (standard IHC practice; not target-specific); Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image A02492-2) | |
| Caveat | Staining has low consistency with RNA expression (HPA tissue IHC) | |
| Regulation | Heat shock drives nuclear-foci translocation (UniProt) | |
| Isoform / epitope | No listed isoforms or processing; one 1–196 chain (UniProt) |
Section 1
Recommended HSPB8 IHC & IF Protocols
The catalog antibody’s IHC-P protocol is accompanied by 3 published HSPB8 immunohistochemistry protocols (PMC5756276; PMC10777032; PMC4688967).
| Sample | Paraffin-embedded mouse pancreas tissues; fixative not specified (datasheet A02492-2) |
| Fixation | Image fixative and duration unreported (datasheet A02492-2); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: Citrate pH 6, 20 min (datasheet A02492-2) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet A02492-2) |
| Primary antibody | Rabbit anti-HSPB8, 0.5-1μg/ml (datasheet A02492-2) |
| Primary incubation | Overnight at 4 °C (datasheet A02492-2) |
| Detection | Streptavidin-biotin complex (SABC), DAB chromogen (datasheet A02492-2) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | HSPB8-positive staining in neuronal cells of cerebral cortex (HPA tissue IHC: High). HPA tissue profile: Cytoplasmic expression in several different tissue types. No signal in the no-primary control. |
💡Decision noteStart with heat-mediated citrate retrieval at pH 6 (datasheet: A02492-2); the published bladder tissue protocol used EDTA retrieval (PMC10777032).
Section 2
What Is the Expected HSPB8 Staining Pattern?
HSPB8 is expected mainly in the cytoplasm, with possible nuclear staining; it has no transmembrane segment (UniProt Q9UJY1 topology and localisation; HPA: cytoplasmic tissue IHC). HPA reports high staining in selected neuronal, epithelial and glandular cells, among others (HPA: tissue IHC). Treat this as a guide to interpretation: the tissue IHC profile is Approved, with low consistency between antibody staining and RNA expression (HPA: reliability description).
| Cytoplasmic staining in cerebral cortex neurons or lung alveolar cells. | This fits reported high staining in both cell types (HPA: High in neuronal cells and alveolar cells). Assess the named cells rather than calling an entire section uniformly positive (general IHC interpretation). |
| Predominantly membrane staining, or nuclear-only staining across a section. | An isolated membrane pattern conflicts with the reported intracellular locations and lack of a transmembrane segment (UniProt Q9UJY1; HPA: cytosol supported). Nuclear staining can occur, so review its accompanying cytoplasmic signal before judging it (UniProt Q9UJY1; HPA: nucleoplasm supported). |
| Strong signal in adipocytes or bone-marrow hematopoietic cells. | Those cells are listed as not detected (HPA: adipocytes and hematopoietic cells). Check for nonspecific antibody binding or endogenous chromogenic activity with appropriate controls (general IHC practice); a discrepant section alone does not identify the cause. |
| Diffuse color over cells and surrounding tissue, without a clear cellular pattern. | This is difficult to assign to HSPB8, whose reported tissue pattern is cytoplasmic (HPA: tissue IHC). Review the no-primary control and detection background before scoring cells (general IHC practice). |
| No staining in an expected high-staining cell population. | Confirm that the expected cells are present and the assay controls worked (general IHC practice). A negative result warrants review of the run; HPA reports high staining in several specific cell populations, but its tissue profile has low antibody–RNA consistency (HPA: tissue IHC and reliability). |
💡Expected HSPB8 appearanceCall a positive result when clear cytoplasmic color is present in the relevant cells, potentially with nuclear signal; examples include high staining in cerebral cortex neurons or lung alveolar cells (HPA: tissue IHC; HPA: cytosol and nucleoplasm supported). Isolated membrane color or diffuse, cell-independent color is suspect (UniProt Q9UJY1 topology; general IHC interpretation).
| Compartment and topology | HSPB8 is reported in cytoplasm and nucleus, with no transmembrane segment (UniProt Q9UJY1). HPA tissue IHC describes cytoplasmic expression, so assess intracellular staining first (HPA: tissue IHC). |
| Choice of tissue control | Cerebral cortex neurons, lung alveolar cells and pancreatic exocrine glandular cells are reported High; adipocytes are Not detected (HPA: tissue IHC). Use the specified cell population when comparing sections, since tissue-level labels alone can obscure which cells are being scored (general IHC interpretation). |
| Strength of tissue evidence | The tissue IHC profile is Approved, with low consistency between antibody staining and RNA expression (HPA: reliability description). Interpret a discordant specimen alongside its controls; do not treat the reported pattern as an absolute result for every section (general IHC interpretation). |
| Antibody evidence | HPA015876 is listed as IHC Approved and ICC Supported (HPA: antibody validation). These are application-specific statuses; they do not establish a target-specific fixation effect or a dilution for a different assay (HPA: supplied antibody record). |
| IF/ICC Q&A: where should signal appear? | Mainly in the cytosol, with additional nucleoplasmic localisation (HPA: subcellular ICC-IF, supported). UniProt also reports movement to nuclear foci during heat shock (UniProt Q9UJY1). These observations guide localisation; this IHC section supplies no IF/ICC protocol. |
| Situation | Likely cause | Next action |
|---|---|---|
| Expected high-staining cells show no chromogenic signal. | The expected cell population may be absent from the field, or the run may have failed (general IHC practice). High staining applies to named cell populations (HPA: tissue IHC). | Locate the reported cells, then check a known-positive section and the run’s detection controls (general IHC practice). Review primary antibody use and retrieval against the assay instructions; no HSPB8-specific retrieval condition is supplied. |
| Color is confined to plasma membranes. | A dominant membrane pattern disagrees with the reported intracellular localisation and lack of a transmembrane segment (UniProt Q9UJY1; HPA: subcellular). | Compare cellular boundaries with the counterstain and check no-primary background (general IHC practice). Reassess the pattern with the validated antibody before scoring it as HSPB8. |
| Nuclei stain, but cytoplasm appears clear. | Nuclear localisation is reported, including nuclear foci during heat shock, while HPA places HSPB8 mainly in cytosol (UniProt Q9UJY1; HPA: subcellular). Nuclear-only IHC therefore needs review. | Check a positive cell population for accompanying cytoplasmic color and compare with a no-primary control (general IHC practice). Record nuclear staining separately rather than assuming it establishes the expected tissue pattern. |
| A reported not-detected cell population stains strongly. | Adipocytes and bone-marrow hematopoietic cells are listed as Not detected (HPA: tissue IHC). Nonspecific binding or endogenous detection activity are possible explanations (general IHC practice). | Verify cell identity and inspect no-primary and reagent controls; address endogenous activity according to the chromogenic detection workflow (general IHC practice). Treat the discrepancy as unresolved until controls support attribution. |
| Color is widespread and obscures cellular boundaries. | Diffuse background can interfere with reading the reported cytoplasmic pattern (HPA: tissue IHC; general IHC practice). Its appearance alone does not establish which assay step caused it. | Inspect no-primary background, blocking, antibody concentration and detection timing using the assay instructions (general IHC practice). Score only cells whose compartment can be distinguished from surrounding color. |
| IHC and IF/ICC appear to disagree on localisation. | HPA tissue IHC reports cytoplasmic expression, while supported ICC-IF also shows nucleoplasm (HPA: tissue IHC; HPA: subcellular ICC-IF). The assays and specimens provide different views (general IHC/IF interpretation). | Compare the cytosolic component first, then document nuclear signal separately (HPA: subcellular ICC-IF). Review each assay’s controls and application-specific antibody status (HPA: IHC Approved; ICC Supported; general IHC/IF practice). |
Experiment evidence
IHC & IF images showing HSPB8 staining
A02492-2 · IHCIHC analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody (A02492-2). HSPB8/Hsp22 was detected in paraffin-embedded section of mouse pancr
A02492-2 · IHCIHC analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody (A02492-2). HSPB8/Hsp22 was detected in paraffin-embedded section of rat pancrea
A02492-2 · IFIF analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody (A02492-2). HSPB8/Hsp22 was detected in immunocytochemical section of MCF7 cells.
A02492 · IFICC staining Hsp22 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with
M02492-2 · IFIF analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody (M02492-2). HSPB8/Hsp22 was detected in immunocytochemical section of A431 cells.
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000152137-HSPB8/tissue/cerebral+cortex#imid_4160772
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000152137-HSPB8/tissue/colon#imid_4160706
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000152137-HSPB8/tissue/liver#imid_4160699
HPA · KidneyHuman Protein Atlas IHC, Kidney. Source: https://www.proteinatlas.org/ENSG00000152137-HSPB8/tissue/kidney#imid_4160700
HPA · ICC-IF MCF-7Human Protein Atlas immunofluorescence, MCF-7, antibody HPA015876; green = HSPB8, blue = nucleus, red = microtubules.
HPA · ICC-IF MCF-7Human Protein Atlas immunofluorescence, MCF-7, antibody HPA015876; green = HSPB8, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for HSPB8 IHC & IF
🧪Run pancreas first; exocrine glandular cells should stain (HPA: High in pancreatic exocrine glandular cells). Run adipose tissue as the negative tissue (HPA: Not detected in adipocytes); on the pancreas slide, use non-exocrine areas as background references, but do not assume those cells are HSPB8-negative without validation (HPA: only exocrine glandular cells specified).
Positive control tissue: Cerebral cortex (Neuronal cells, HPA High)
Negative control tissue: Adipose tissue (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show HSPB8 in MCF-7, U-251MG, U2OS, with annotated localisation: Cytosol (supported) (HPA subcellular).
Technical controls: Run a no-primary, secondary-only control, a concentration-matched rabbit IgG isotype control matched to the primary’s clonality, and a matched HSPB8 knockout biological negative (selected-SKU caption: rabbit primary; standard IHC controls). For pancreatic sections developed with SABC/DAB, quench endogenous peroxidase and check for endogenous biotin background (selected-SKU caption: pancreas, SABC/DAB; standard IHC practice).
⚠️Feasibility: A target-specific fixation window or fixation effect is unreported, and the exact selected-SKU paraffin-section caption does not state the fixative (selected-SKU caption: fixative not stated). Heat-mediated citrate retrieval at pH 6 for 20 min provides a paraffin IHC starting condition; whether HSPB8 detection depends on that retrieval is unreported (selected-SKU caption: citrate retrieval). Whether frozen sections or IF are easier is unreported; pancreatic SABC/DAB staining requires attention to endogenous biotin and peroxidase background (selected-SKU caption: pancreas, SABC/DAB; standard IHC practice).
HPA tissue IHC evidence for HSPB8
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Approved — Low consistency between antibody staining and RNA expression data.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebral cortex | Neuronal cells | High | Protein (IHC) | HPA → |
| Cervix | Squamous epithelial cells | High | Protein (IHC) | HPA → |
| Esophagus | Squamous epithelial cells | High | Protein (IHC) | HPA → |
| Lung | Alveolar cells | High | Protein (IHC) | HPA → |
| Pancreas | Exocrine glandular cells | High | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | Adipocytes | Not detected | Protein (IHC) | HPA → |
| Bone marrow | Hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Ovary | Ovarian stroma cells | Not detected | Protein (IHC) | HPA → |
| Spleen | Cells in red pulp | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced HSPB8 IHC Tips
Troubleshoot HSPB8 staining in paraffin sections using the catalog antibody’s tissue image, expected localisation, and appropriate controls.
What retrieval should I try first when HSPB8 staining is weak?
Start with heat-mediated citrate retrieval at pH 6 for 20 minutes on paraffin sections (datasheet A02492-2). The selected image used this condition to detect HSPB8 in mouse pancreas, but its fixative was not reported (caption A02492-2). Keep section thickness, heating, and cooling consistent across test and control slides so retrieval differences remain interpretable (standard IHC practice). Check whether weak staining persists in the expected exocrine glandular cells before changing retrieval conditions (HPA: High in pancreatic exocrine glandular cells). If it does, test an alternative retrieval buffer as a fallback on matched sections, watching for tissue damage and increased background (standard IHC practice).
Could fixation explain inconsistent HSPB8 staining across paraffin blocks?
Possibly, but target-specific fixation sensitivity is unknown: the selected paraffin-section image does not state its fixative (caption A02492-2). Record each block’s fixative and fixation duration, then compare sections using the same citrate pH 6 retrieval and detection conditions (datasheet A02492-2; standard IHC practice). Differences in fixation or tissue processing can change antigen accessibility and morphology, so examine both before attributing intensity changes to HSPB8 abundance (standard IHC practice). Include a reference block in each run and inspect cellular localisation alongside staining intensity (standard IHC practice). Do not use HPA tissue patterns or HSPB8 modifications to infer a preferred fixative (HPA: tissue IHC; UniProt Q9UJY1: modified residues).
Should HSPB8 staining appear in nuclei or only in cytoplasm?
Expect predominantly cytoplasmic staining in routine tissue sections; HPA reports cytoplasmic expression across several tissues (HPA: tissue IHC). HSPB8 is also assigned to the nucleus and can move into nuclear foci during heat shock (UniProt Q9UJY1: subcellular location). Interpret nuclear staining in light of the specimen’s stress context and confirm that the signal lies within intact cells (UniProt Q9UJY1: subcellular location; standard IHC practice). A membrane-only outline would be unexpected because HSPB8 has no transmembrane segment (UniProt Q9UJY1: topology). Compare compartments in the same cell type and inspect the counterstain before scoring a nuclear shift (standard IHC practice).
How should I investigate discordant staining if the antibody epitope is unmapped?
The supplied record lists a single 196-amino-acid HSPB8 chain and no isoforms, so an isoform-specific explanation is unsupported here (UniProt Q9UJY1: processing, isoforms). Its small heat shock protein domain spans residues 74–185, while reported modified residues include 24, 57, 71, and 78 (UniProt Q9UJY1: domains, modified residues). The catalog image does not identify the antibody’s binding epitope (caption A02492-2). Therefore, changes in staining cannot be assigned to a particular modification or epitope without additional validation (UniProt Q9UJY1: modified residues; standard IHC practice). Compare matched sections under identical retrieval and detection conditions, then seek orthogonal evidence for discordant samples (standard IHC practice).
How can I check HSPB8 localisation by multiplex IF?
Use a validated marker for the expected cell type alongside HSPB8; exocrine glandular cells are a supported pancreatic reference population (HPA: High in pancreatic exocrine glandular cells). Choose spectrally separated fluorophores and consider a far-red HSPB8 channel when tissue autofluorescence obscures shorter wavelengths (standard IF practice). HSPB8 is intracellular and lacks a transmembrane segment, so permeabilisation must allow antibody access to cytosolic and potentially nuclear epitopes (UniProt Q9UJY1: topology, subcellular location). Check each channel alone and include secondary-only controls before interpreting overlap (standard IF practice). HPA supports cytosolic and additional nucleoplasmic localisation in cell images, but that pattern alone does not establish tissue colocalisation (HPA: subcellular).
What should I check when DAB signal spreads beyond expected cells?
The selected image used 10% goat serum, 1 µg/mL primary antibody, a biotinylated secondary, and an avidin–biotin detection complex with DAB (caption A02492-2). Check a no-primary slide and inspect whether staining follows tissue edges, damaged areas, or the expected cellular pattern (standard IHC practice). Include a peroxidase block for chromogenic detection and assess endogenous biotin interference when using avidin–biotin detection (standard IHC practice). If background persists, titrate the primary around the documented concentration while keeping retrieval and development consistent (caption A02492-2; standard IHC practice). Judge any improvement against cytoplasmic signal in relevant cells, rather than reduced overall darkness alone (HPA: tissue IHC; standard IHC practice).
How should I score HSPB8 across sections with different cell mixtures? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell population and tissue region before scoring; HPA reports High staining in pancreatic exocrine glandular cells but Not detected in adipocytes (HPA: tissue IHC). For cellular staining, record the percentage of positive cells and an intensity-weighted H-score on a 0–300 scale (standard IHC practice). If counting discrete positive cells, report density per mm² of evaluable tissue as a separate measure (standard IHC practice). Normalise comparisons to the number of evaluable cells or measured tissue area within the same cell type, excluding damaged regions (standard IHC practice). Use consistent illumination, thresholds, counterstaining, and reference controls across slides (standard IHC practice).
Which HSPB8 patterns suggest genuine staining rather than artefact?
A plausible positive pattern is intracellular and mainly cytoplasmic in an expected cell population; HPA reports High staining in pancreatic exocrine glandular cells (HPA: tissue IHC). Nuclear foci may fit heat-shock-associated redistribution, but require an appropriate stress context and intact nuclear morphology (UniProt Q9UJY1: subcellular location; standard IHC practice). Treat membrane-only signal cautiously because HSPB8 has no transmembrane segment (UniProt Q9UJY1: topology). Edge staining, necrotic areas, and signal on a no-primary slide warrant investigation for processing or detection artefacts, including endogenous enzyme activity (standard IHC practice). Finally, HPA marks its tissue staining Approved while reporting low consistency with RNA expression, so corroborate unexpected patterns independently (HPA: reliability description).
Boster reagents
Best HSPB8 / Heat shock protein beta-8 IHC Antibodies
Anti-HSPB8 antibodies have IHC images from mouse and rat pancreas and human lung and mammary cancer (A02492-2 image captions), plus ICC/IF images from HeLa and A431 cells (A02492 and M02492-2 image captions).
Real IHC data
Anti-HSPB8/Hsp22 Antibody ®
Cat # A02492-2
Real IF data
Anti-HSP22 HSPB8 Antibody
Cat # A02492
Real IF data
Anti-HSPB8/Hsp22 Antibody ® (monoclonal, 7D8)
Cat # M02492-2
A02492-2 shows paraffin-section IHC in mouse and rat pancreas and human lung and mammary cancer, and its catalog lists human, mouse and rat reactivity (A02492-2 image captions; catalog applications and reactivity). A02492 shows ICC/IF in HeLa cells, while M02492-2 shows ICC/IF in A431 cells (A02492 and M02492-2 image captions).
Which to pick: For tissue IHC, choose A02492-2: its own images document paraffin sections with citrate retrieval at pH 6 and 1 μg/ml primary antibody; the fixative is unreported (A02492-2 IHC image captions). For IF/ICC, choose A02492 for a rabbit polyclonal with human, mouse and rat listed reactivity, or M02492-2 for a mouse monoclonal with human and rat listed reactivity (A02492 and M02492-2 catalog applications, host, clone and reactivity). For cross-species tissue IHC, A02492-2 has image evidence in all three listed species (A02492-2 IHC image captions; catalog reactivity).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry Q9UJY1 (HSPB8_HUMAN, Heat shock protein beta-8).
- Human Protein Atlas. HSPB8 tissue IHC expression (reliability: Approved).
- Human Protein Atlas. HSPB8 subcellular location (ICC-IF): Mainly localized to the cytosol. In addition localized to the nucleoplasm..
- Human Protein Atlas. HSPB8 antibody validation summary (1 antibodies).
- A knock-in/knock-out mouse model of HSPB8-associated distal hereditary motor neuropathy and myopathy reveals toxic gain-of-function of mutant Hspb8. Acta neuropathologica 2018 — PMC5756276.
- HSPB8 Facilitates the Oncogenesis and Advancement of Bladder Cancer via Activation of HSP27. Journal of Cancer 2024 — PMC10777032.
- Small heat shock proteins are induced during multiple sclerosis lesion development in white but not grey matter. Acta neuropathologica communications 2015 — PMC4688967.
- Heat shock proteins are differentially expressed in brain and spinal cord: implications for multiple sclerosis. Clinical and experimental immunology 2018 — PMC6194336.
- PubMed PMID:11085516 — UniProt-cited evidence.
- PubMed PMID:11342557 — UniProt-cited evidence.
- PubMed PMID:10833516 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for HSPB8 (Heat shock protein beta-8). Expected localisation Cytoplasmic staining in several tissues (HPA tissue IHC). Antigen retrieval: Citrate pH 6 HIER, heat-mediated (datasheet A02492-2). Validated IHC antibody: A02492-2 at 0.5-1μg/ml (the product's own IHC validation image is shown). Controls (HPA): positive Cerebral cortex (Neuronal cells); negative Adipose tissue. IHC-P workflow on paraffin sections; fixative unspecified. Protocol steps: 1. Sample: Paraffin-embedded mouse pancreas tissues; fixative not specified (datasheet A02492-2); 2. Fixation: Image fixative and duration unreported (datasheet A02492-2); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: Citrate pH 6 , 20 min (datasheet A02492-2); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet A02492-2); 8. Primary antibody: Rabbit anti-HSPB8, 0.5-1μg/ml (datasheet A02492-2); 9. Primary incubation: Overnight at 4 °C (datasheet A02492-2). Watch for: Fixation: Keep tissue fixation consistent (standard IHC practice; not target-specific); Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image A02492-2); Caveat: Staining has low consistency with RNA expression (HPA tissue IHC); Isoform / epitope: No listed isoforms or processing; one 1–196 chain (UniProt); Regulation: Heat shock drives nuclear-foci translocation (UniProt). Evidence: UniProt Q9UJY1, HPA tissue IHC · Approved, HPA ICC-IF · Mainly localized to the cytosol. In addition localized to the…, Datasheet A02492-2, PMC5756276, PMC10777032. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/HSPB8/immunohistochemistry-ihc-guide.