Product Info Summary
SKU: | A02492-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HSPB8/Hsp22 Antibody Picoband™
View all HSPB8/HSP22 Antibodies
SKU/Catalog Number
A02492-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSPB8/Hsp22 Antibody Picoband™ catalog # A02492-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSPB8/Hsp22 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02492-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HSPB8/Hsp22 recombinant protein (Position: M1-T196). Human HSPB8/Hsp22 shares 94.4% and 95.4% amino acid (aa) sequence identity with mouse and rat HSPB8/Hsp22, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A02492-2 is reactive to HSPB8 in Human, Mouse, Rat
Applications
A02492-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
22 kDa
Calculated molecular weight
21.604kDa
Background of HSPB8/HSP22
Heat shock protein beta-8 is a protein that in humans is encoded by the HSPB8 gene. The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody (A02492-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: rat H9C2 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: mouse C2C12 whole cell lysates,
Lane 6: mouse skeletal muscle tissue lysates,
Lane 7: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB8/Hsp22 antigen affinity purified polyclonal antibody (Catalog # A02492-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB8/Hsp22 at approximately 22 kDa. The expected band size for HSPB8/Hsp22 is at 22 kDa.
Click image to see more details
Figure 2. IHC analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody (A02492-2).
HSPB8/Hsp22 was detected in paraffin-embedded section of mouse pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSPB8/Hsp22 Antibody (A02492-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody (A02492-2).
HSPB8/Hsp22 was detected in paraffin-embedded section of rat pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSPB8/Hsp22 Antibody (A02492-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody (A02492-2).
HSPB8/Hsp22 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSPB8/Hsp22 Antibody (A02492-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody (A02492-2).
HSPB8/Hsp22 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSPB8/Hsp22 Antibody (A02492-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IF analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody (A02492-2).
HSPB8/Hsp22 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HSPB8/Hsp22 Antibody (A02492-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 7. Flow Cytometry analysis of U87 cells using anti-HSPB8/Hsp22 antibody (A02492-2).
Overlay histogram showing U87 cells stained with A02492-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSPB8/Hsp22 Antibody (A02492-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For HSPB8 (Source: Uniprot.org, NCBI)
Gene Name
HSPB8
Full Name
Heat shock protein beta-8
Weight
21.604kDa
Superfamily
small heat shock protein (HSP20) family
Alternative Names
Alpha-crystallin C chain; CMT2L; CRYAC; DHMN2; E2IG1; E2IG1E2-induced gene 1 protein; H11; H11DHMN2; heat shock 22kDa protein 8; heat shock 27kDa protein 8; heat shock protein beta-8; HMN2; HMN2A; HSP22; HSP22CMT2L; HSPB8; Protein kinase H11; Small stress protein-like protein HSP22 HSPB8 CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22 heat shock protein family B (small) member 8 heat shock protein beta-8|E2-induced gene 1 protein|alpha-crystallin C chain|heat shock 22kDa protein 8|heat shock 27kDa protein 8|protein kinase H11|small stress protein-like protein HSP22
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPB8, check out the HSPB8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPB8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSPB8/Hsp22 Antibody Picoband™ (A02492-2)
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Customer Q&As
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1 Customer Q&As for Anti-HSPB8/Hsp22 Antibody Picoband™
Question
We are currently using anti-HSPB8/Hsp22 antibody A02492-2 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
H. Singh
Verified customer
Asked: 2017-01-02
Answer
The anti-HSPB8/Hsp22 antibody (A02492-2) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-01-02