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HSPH1 / Heat shock protein 105 kDa · IHC design guide
Design Immunohistochemistry for HSPH1
Plan HSPH1 paraffin-section IHC around the cytoplasmic tissue pattern (HPA tissue IHC). Use tissue controls and interpret staining cautiously because antibody staining and RNA expression show very low consistency (HPA tissue IHC).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
HSPH1 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasmic staining in most tissues (HPA tissue IHC) | |
| Staining pattern | Cytoplasmic signal in cells across most tissues (HPA tissue IHC) | |
| Antigen retrieval | EDTA pH 8.0 HIER, heat-mediated (datasheet M04168) | |
| Positive control | Appendix+4 more · see all | |
| Negative control | Adipose tissue+4 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Keep fixation conditions consistent across sections. (standard IHC practice; not target-specific) | |
| Caveat | Staining and RNA show very low consistency (HPA tissue IHC) | |
| Regulation | Higher expression in testis and cancer cells (UniProt) | |
| Isoform / epitope | 4 isoforms; assess antibody epitope coverage (UniProt) |
Section 1
Recommended HSPH1 IHC & IF Protocols
The catalog antibody’s IHC-P protocol uses EDTA pH 8.0 retrieval (datasheet: M04168). The published protocols below report HSPH1 staining in esophageal, liver, lung, and head and neck tissues (PMC13339864; PMC12375714; PMC12088961; PMC6139602).
| Sample | Paraffin-embedded human breast cancer tissue; fixative not specified (datasheet M04168) |
| Fixation | Image fixative and duration unreported (datasheet M04168); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: EDTA pH 8.0 (datasheet M04168); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet M04168) |
| Primary antibody | Rabbit monoclonal (clone IOG-8) anti-HSPH1, 1:50 (datasheet M04168) |
| Primary incubation | Overnight at 4 °C (datasheet M04168) |
| Detection | HRP-conjugated secondary, DAB chromogen (datasheet M04168) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | HSPH1-positive staining in glandular cells of appendix (HPA tissue IHC: High). HPA tissue profile: Cytoplasmic expression in most tissues. No signal in the no-primary control. |
💡Decision noteStart with heat-mediated EDTA pH 8.0 for the catalog antibody (datasheet: M04168); the published protocols used EDTA pH 9.0, citrate pH 6.0, or an unspecified autoclave retrieval buffer (PMC13339864; PMC12375714; PMC6139602; PMC12088961).
Section 2
What Is the Expected HSPH1 Staining Pattern?
HSPH1 is a cytoplasmic protein without a transmembrane segment (UniProt Q92598: subcellular location and topology). In paraffin-section IHC, expect cytoplasmic staining in responsive cells, including appendix and colon glandular cells, bronchial respiratory epithelial cells, and cerebral cortex neurons (HPA: High in these cells). Treat tissue comparisons cautiously: HPA rates its tissue IHC evidence Uncertain because antibody staining agrees poorly with RNA data (HPA: reliability description).
| Glandular or respiratory epithelial cells show cytoplasmic chromogen, while adjacent cells vary in intensity. | This fits the reported compartment and selected high-staining cell populations (UniProt Q92598: Cytoplasm; HPA: High in appendix and colon glandular cells and bronchial respiratory epithelial cells). Score the named cell type and compartment, not whole-section darkness (general IHC practice). |
| Strong signal appears mainly at cell borders or in nuclei, with little cytoplasmic staining. | A border-dominant pattern conflicts with the reported cytoplasmic location and absence of a transmembrane segment (UniProt Q92598: location and topology). Nuclear signal needs caution rather than automatic rejection because ICC-IF also reports nucleoplasm (HPA: additional nucleoplasmic location). |
| Cells reported as unstained, such as adipocytes or cardiomyocytes, stain as strongly as selected positive cells. | That conflicts with the reported tissue IHC pattern (HPA: Not detected in adipocytes and cardiomyocytes). Check cell identification and controls for cross-reactivity or endogenous detection activity (general IHC practice); HPA's Uncertain IHC rating prevents an absolute negative call (HPA: reliability). |
| Chromogen coats the section diffusely and obscures cell boundaries. | Diffuse background cannot establish HSPH1 localization (general IHC practice). Compare a reagent-omission control and inspect blocking, washes, and detection chemistry (general IHC practice); do not score background as the cytoplasmic pattern reported for HSPH1 (UniProt Q92598: Cytoplasm). |
| No cytoplasmic signal appears in a well-preserved appendix or colon glandular compartment. | Those cells are reported High by tissue IHC (HPA: appendix and colon glandular cells), so first check assay execution and the IHC-validated antibody's documented conditions (general IHC practice). A negative result alone does not establish absent HSPH1 because HPA rates tissue IHC reliability Uncertain (HPA: reliability). |
💡Expected HSPH1 appearanceA credible positive is cell-resolved cytoplasmic chromogen in reported High glandular or epithelial cells (UniProt Q92598: Cytoplasm; HPA: High in appendix, colon, and bronchus); dominant border staining or uniform tissue-wide haze warrants artifact checks (UniProt Q92598: no transmembrane segment; general IHC practice).
| Cell type and reference tissue | HPA reports High staining in appendix, colon, duodenum, and epididymis glandular cells, but Not detected in adipocytes and cardiomyocytes (HPA: tissue IHC). Compare matching cells rather than treating an entire organ as uniformly positive or negative (general IHC practice). |
| Evidence confidence | The tissue IHC profile is rated Uncertain because staining and RNA expression show very low consistency (HPA: reliability description). HPA lists IHC as Uncertain for HPA028675 and CAB002060 (HPA: antibody validation); interpret unexpected positives and negatives with controls. |
| Isoform and antigen scope | Four isoforms are listed—Alpha, Beta, 3, and 4 (UniProt Q92598: isoforms). The supplied evidence does not map an IHC epitope to those forms (UniProt Q92598: record); a stain cannot identify one isoform without separate validation (general IHC practice). |
| Processing and compartment | UniProt reports no signal peptide or propeptide, a chain spanning residues 2–858, and no transmembrane segment (UniProt Q92598: processing and topology). These annotations support a cytoplasmic expectation, but do not establish an antigen-retrieval requirement or fixation sensitivity. |
| IF/ICC Q&A: Does nuclear fluorescence invalidate the result? | No: HPA reports mainly cytosolic signal with additional nucleoplasmic localization in ICC-IF (HPA: subcellular summary). That observation informs compartment interpretation; ICC-IF has its own guide and does not provide an IHC protocol here. |
| Situation | Likely cause | Next action |
|---|---|---|
| No signal in appendix or colon glandular cells. | These are reported High, but tissue IHC reliability is Uncertain (HPA: tissue IHC and reliability); failed detection and biological variation remain possible (general IHC practice). | Check section integrity, antibody and detection steps, and a suitable positive-control section (general IHC practice); document the tested conditions before interpreting absence. |
| Chromogen appears in adipocytes or cardiomyocytes. | These cells are reported Not detected (HPA: tissue IHC); cross-reactivity or endogenous detection activity can also produce apparent signal (general IHC practice). | Review cell identity, use the appropriate detection-control section, and compare compartment and intensity with a reported High cell population (general IHC practice; HPA: tissue IHC). |
| A crisp membrane rim dominates staining. | HSPH1 is cytoplasmic and has no transmembrane segment (UniProt Q92598: location and topology); a membrane-dominant readout is discordant. | Inspect morphology and reagent-omission controls, then reassess antibody specificity with independent validation where available (general IHC practice). |
| Diffuse brown background hides cytoplasmic detail. | Nonspecific reagent deposition or endogenous detection activity can obscure compartment assignment (general IHC practice). | Compare omission controls, review blocking and washing, and adjust detection conditions using the antibody's IHC documentation (general IHC practice); score only cell-resolved signal. |
| Brain staining differs across regions or cell types. | UniProt describes lower expression in most brain regions except cerebellum, while HPA reports High in caudate and cortex neurons and cerebellar Purkinje cells (UniProt Q92598: tissue specificity; HPA: tissue IHC). | Record the specific region and cell type; interpret differences alongside HPA's Uncertain tissue IHC rating rather than assuming uniform brain staining (HPA: reliability; general IHC practice). |
| Nuclear staining accompanies cytoplasmic signal. | ICC-IF reports an additional nucleoplasmic location, while UniProt gives Cytoplasm (HPA: subcellular summary; UniProt Q92598: subcellular location). | Document both compartments and check IHC controls before calling nuclear chromogen specific; use the separate IF/ICC guide for fluorescence assay design (general IHC practice). |
Experiment evidence
IHC & IF images showing HSPH1 staining
M04168 · IHCIHC analysis of HSPH1 using anti-HSPH1 antibody (M04168). HSPH1 was detected in a paraffin-embedded section of human breast cancer tissue. H
M04168 · IHCIHC analysis of HSPH1 using anti-HSPH1 antibody (M04168). HSPH1 was detected in a paraffin-embedded section of human stomach cancer tissue.
M04168-1 · IHCIHC analysis of HSPH1 using anti-HSPH1 antibody (M04168-1). HSPH1 was detected in paraffin-embedded section of human lung cancer tissues. He
M04168-1 · IHCIHC analysis of HSPH1 using anti-HSPH1 antibody (M04168-1). HSPH1 was detected in paraffin-embedded section of human mammary cancer tissues.
M04168-1 · IFIF analysis of HSPH1 using anti-HSPH1 antibody (M04168-1). HSPH1 was detected in immunocytochemical section of A431 cell. Enzyme antigen ret
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000120694-HSPH1/tissue/cerebral+cortex#imid_891632
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000120694-HSPH1/tissue/colon#imid_891558
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000120694-HSPH1/tissue/liver#imid_891559
HPA · KidneyHuman Protein Atlas IHC, Kidney. Source: https://www.proteinatlas.org/ENSG00000120694-HSPH1/tissue/kidney#imid_891568
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody CAB002060; green = HSPH1, blue = nucleus, red = microtubules.
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody CAB002060; green = HSPH1, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for HSPH1 IHC & IF
🧪Run appendix first and confirm staining in its glandular cells (HPA: High in appendix glandular cells); use adipose tissue adipocytes as the biological tissue negative (HPA: Not detected in adipocytes). On the appendix slide, assess non-glandular cells against the control slides for background staining, without assuming those cells are HSPH1-negative (HPA: appendix result specifies glandular cells).
Positive control tissue: Appendix (Glandular cells, HPA High)
Negative control tissue: Adipose tissue (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show HSPH1 in A-431, U-251MG, U2OS, U2OS, siRNA 2 (10x), U2OS, siRNA 2 (40x), U2OS, scrambled (10x), U2OS, scrambled (40x), NIH 3T3, with annotated localisation: Cytosol (enhanced) (HPA subcellular).
Technical controls: Include a no-primary, secondary-only control and a rabbit control immunoglobulin matched to the primary antibody’s isotype and clonality format (caption: rabbit anti-HSPH1); use HSPH1 knockout material, if available, to test target specificity (standard IHC practice). Block endogenous peroxidase and check for DAB background in the appendix section (standard chromogenic IHC practice).
⚠️Feasibility: No target-specific fixation window or fixation effect is reported in the supplied evidence, and the selected M04168 paraffin-section caption does not state the fixative (caption: fixative unreported). The caption uses heat retrieval in EDTA at pH 8.0, but does not establish that retrieval is required; it supports a paraffin IHC starting condition, while the supplied IF evidence concerns ICC localization and does not show whether frozen sections or IF are easier (caption: EDTA retrieval; HPA: ICC-IF images). In appendix, distinguish cellular staining from DAB background in gland lumens (standard chromogenic IHC practice).
HPA tissue IHC evidence for HSPH1
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Uncertain — Very low consistency between antibody staining and RNA expression data. Pending external verification. Significant inter-individual differences was observed between vagina RNA expression values.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | Glandular cells | High | Protein (IHC) | HPA → |
| Bronchus | Respiratory epithelial cells | High | Protein (IHC) | HPA → |
| Caudate | Neuronal cells | High | Protein (IHC) | HPA → |
| Cerebellum | Purkinje cells | High | Protein (IHC) | HPA → |
| Cerebral cortex | Neuronal cells | High | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | Adipocytes | Not detected | Protein (IHC) | HPA → |
| Heart muscle | Cardiomyocytes | Not detected | Protein (IHC) | HPA → |
| Ovary | Ovarian stroma cells | Not detected | Protein (IHC) | HPA → |
| Skeletal muscle | Myocytes | Not detected | Protein (IHC) | HPA → |
| Smooth muscle | Smooth muscle cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced HSPH1 IHC Tips
Troubleshoot HSPH1 staining in paraffin sections by checking retrieval, cytoplasmic localisation, controls and cell-specific scoring before interpreting differences in signal.
Which retrieval conditions should I try first for weak HSPH1 staining?
Start with heat-mediated retrieval in EDTA at pH 8.0 (datasheet M04168). This is the condition used for the catalog antibody in a paraffin section of human breast cancer; that caption also reports a 1:50 primary dilution and overnight incubation at 4°C (datasheet M04168). If staining is weak, compare retrieval heating and cooling consistently across adjacent sections while keeping the antibody and detection conditions fixed (standard IHC practice). Include a known staining reference section in each run, because HSPH1 staining varies by cell type (HPA: tissue IHC). Do not assign a fixation-related cause from this caption: its fixative is unreported (datasheet M04168).
Could fixation explain inconsistent HSPH1 staining between paraffin sections?
Target-specific fixation sensitivity is unknown: the selected tissue-IHC caption identifies a paraffin section but does not state its fixative (datasheet M04168). Record each specimen’s fixative, fixation duration and processing history before comparing staining across blocks (standard IHC practice). On adjacent sections, hold retrieval at EDTA pH 8.0 and the primary at 1:50 while assessing whether the discrepancy follows a block or a staining run (datasheet M04168; standard IHC practice). Examine tissue preservation and section integrity alongside staining intensity, since processing differences can confound IHC comparisons (standard IHC practice). Neither the reported tissue pattern nor HSPH1 topology establishes a fixation effect (HPA: tissue IHC; UniProt Q92598 topology).
Where should convincing HSPH1 staining appear in tissue cells?
Expect predominantly cytoplasmic staining in paraffin sections (UniProt Q92598: cytoplasm; HPA: cytoplasmic expression in most tissues). HPA also reports mainly cytosolic localisation with an additional nucleoplasmic signal in cell imaging, so a nuclear component warrants assessment alongside the cytoplasm (HPA: subcellular localisation). HSPH1 has no annotated transmembrane segment or signal peptide; isolated crisp membrane staining therefore needs independent validation (UniProt Q92598 topology and processing). Compare compartments within intact cells using a counterstain and inspect a no-primary control for detection background (standard IHC practice). Judge localisation in the relevant cell population, since HPA reports high glandular-cell staining in appendix and colon but no detected staining in adipocytes (HPA: tissue IHC).
Could isoforms or epitope accessibility account for discordant HSPH1 staining?
HSPH1 has 4 annotated isoforms—Alpha, Beta, 3 and 4—but the supplied antibody caption does not identify the epitope (UniProt Q92598: isoforms; datasheet M04168). Therefore, do not infer which isoforms the catalog antibody detects in paraffin sections without epitope mapping or direct validation (UniProt Q92598: isoforms; datasheet M04168). Annotated modifications include acetylation and phosphorylation, including phosphoserines at positions 509, 510 and 557; their effect on this antibody’s staining is unknown (UniProt Q92598: modified residues). If two antibodies disagree, compare their disclosed immunogen regions, staining compartments and matched control sections (standard IHC practice). Keep EDTA pH 8.0 retrieval constant during that comparison (datasheet M04168).
How should I assess HSPH1 by IF alongside this IHC guide?
Treat IF/ICC as a separate validation task: the selected M04168 evidence describes chromogenic staining of a paraffin section, not fluorescence imaging (datasheet M04168). For multiplex IF, pair HSPH1 with a marker identifying the expected cell type, such as a glandular-cell marker when examining appendix, and confirm that signals occupy the same cells (HPA: high in appendix glandular cells; standard IF practice). Select spectrally separated fluorophores and consider a far-red HSPH1 channel when tissue autofluorescence interferes with shorter wavelengths (standard IF practice). Permeabilise for access to cytosolic HSPH1 and assess nuclear access if measuring its additional nucleoplasmic signal; no membrane-sided epitope is indicated (HPA: subcellular localisation; UniProt Q92598 topology; standard IF practice). Use single-stain and no-primary controls to check bleed-through and background (standard IF practice).
How can I distinguish HSPH1 signal from diffuse DAB background?
The selected IHC example used 10% goat serum blocking, a peroxidase-conjugated secondary and DAB detection (datasheet M04168). Add a peroxidase block as a general chromogenic IHC step, and compare no-primary and secondary-only controls with the stained section (standard IHC practice). If diffuse colour persists, review blocking, washes, primary concentration and DAB development while changing one condition at a time (standard IHC practice). The caption’s 1:50 primary dilution and overnight incubation at 4°C provide documented starting conditions, not proof that every tissue will have low background (datasheet M04168). Prioritise intact-cell cytoplasmic signal over colour deposited on tissue edges or damaged areas (UniProt Q92598: cytoplasm; standard IHC practice).
How should I score HSPH1 IHC across heterogeneous specimens? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell population and cytoplasmic scoring compartment before reviewing outcomes, because HSPH1 expression differs across tissue cell types (HPA: tissue IHC; UniProt Q92598: cytoplasm). Record the percentage of positive viable target cells and, when intensity matters, calculate an H-score from percentages at intensities 0–3, giving a range of 0–300 (standard IHC practice). For spatial comparisons, report positive-cell density per mm² of evaluable tissue with the same positivity threshold (standard IHC practice). Normalise counts to viable target-cell number or evaluable target-cell area, and apply identical imaging and scoring settings across sections (standard IHC practice). Report replicate blocks or cases and control performance because HPA rates its tissue-IHC reliability uncertain (HPA: tissue IHC).
What findings support a true HSPH1-positive IHC result?
A credible result shows reproducible staining in intact cells, chiefly in cytoplasm, with an appropriate low-signal no-primary control (UniProt Q92598: cytoplasm; standard IHC practice). Check cell identity: HPA reports high staining in appendix glandular cells and no detected staining in adipocytes, although its overall tissue-IHC reliability is uncertain (HPA: tissue IHC). Isolated membrane outlines, strong section-edge staining or colour concentrated in necrotic regions should prompt review of localisation and tissue integrity (UniProt Q92598 topology; standard IHC practice). Compare staining before and after a peroxidase block to assess endogenous enzyme contribution to DAB signal (standard IHC practice). Interpret between-sample differences cautiously because the HPA tissue profile has very low agreement between staining and RNA data (HPA: reliability description).
Boster reagents
Best HSPH1 / Heat shock protein 105 kDa IHC Antibodies
Anti-HSPH1 antibodies have IHC images from human tissue and rat brain, plus IF/ICC images from human cells (catalog image captions); listed reactivity spans human, mouse, and rat (catalog reactivity).
Real IHC data
Anti-Hsp105 Rabbit Monoclonal Antibody
Cat # M04168
Real IHC data
Anti-Hsp105/HSPH1 Antibody ® (monoclonal, 3D10)
Cat # M04168-1
M04168 renders with paraffin-section IHC images from human breast and stomach tissue and rat brain (M04168 IHC captions). M04168-1 renders with paraffin-section IHC images from human lung, mammary, and intestinal cancer tissue and IF/ICC images from A431 and MCF7 cells (M04168-1 image captions).
Which to pick: For tissue IHC and cross-species work, choose rabbit monoclonal M04168: its paraffin-section images cover human tissue and rat brain, and its listed reactivity includes mouse (M04168 IHC captions; catalog host, clone, and reactivity). For human IF/ICC, choose mouse monoclonal M04168-1 because its IF/ICC images show A431 and MCF7 cells (M04168-1 IF captions; catalog host and clone). The IHC captions for both SKUs describe paraffin sections; the fixative is unreported (M04168 and M04168-1 IHC captions).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry Q92598 (HS105_HUMAN, Heat shock protein 105 kDa).
- Human Protein Atlas. HSPH1 tissue IHC expression (reliability: Uncertain).
- Human Protein Atlas. HSPH1 subcellular location (ICC-IF): Mainly localized to the cytosol. In addition localized to the nucleoplasm..
- Human Protein Atlas. HSPH1 antibody validation summary (3 antibodies).
- Single-cell and bulk omics uncover fibroblast heterogeneity and HSPH1 as a key driver in Barrett's esophagus to esophageal adenocarcinoma progression. Cancer cell international 2026 — PMC13339864.
- Heat shock protein family H member 1 HSPH1 expression correlates with progression and prognosis of hepatocellular carcinoma. Scientific reports 2025 — PMC12375714.
- Correlation of heat shock protein 1 expression with progression and prognosis of non-small cell lung cancer. Frontiers in oncology 2025 — PMC12088961.
- Identification of potential biomarkers of head and neck squamous cell carcinoma using iTRAQ based quantitative proteomic approach. Data in brief 2018 — PMC6139602.
- PubMed PMID:9931472 — UniProt-cited evidence.
- PubMed PMID:9610721 — UniProt-cited evidence.
- PubMed PMID:9039502 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for HSPH1 (Heat shock protein 105 kDa). Expected localisation Cytoplasmic staining in most tissues (HPA tissue IHC). Antigen retrieval: EDTA pH 8.0 HIER, heat-mediated (datasheet M04168). Validated IHC antibody: M04168 at 1:50 (the product's own IHC validation image is shown). Controls (HPA): positive Appendix (Glandular cells); negative Adipose tissue. IHC-P workflow on paraffin sections; fixative unspecified. Protocol steps: 1. Sample: Paraffin-embedded human breast cancer tissue; fixative not specified (datasheet M04168); 2. Fixation: Image fixative and duration unreported (datasheet M04168); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: EDTA pH 8.0 (datasheet M04168); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet M04168); 8. Primary antibody: Rabbit monoclonal (clone IOG-8) anti-HSPH1, 1:50 (datasheet M04168); 9. Primary incubation: Overnight at 4 °C (datasheet M04168). Watch for: Fixation: Keep fixation conditions consistent across sections. (standard IHC practice; not target-specific); Caveat: Staining and RNA show very low consistency (HPA tissue IHC); Isoform / epitope: 4 isoforms; assess antibody epitope coverage (UniProt); Regulation: Higher expression in testis and cancer cells (UniProt). Evidence: UniProt Q92598, HPA tissue IHC · Uncertain, HPA ICC-IF · Mainly localized to the cytosol. In addition localized to the…, Datasheet M04168, PMC13339864, PMC12375714. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/HSPH1/immunohistochemistry-ihc-guide.