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HSPH1 / Heat shock protein 105 kDa · IHC design guide
Design Immunohistochemistry for HSPH1
Plan HSPH1 chromogenic IHC on paraffin sections using its expected cytoplasmic location and tissue staining profile (UniProt; HPA tissue IHC). The guide highlights controls and interpretation limits because the HPA rates its tissue staining evidence uncertain (HPA tissue IHC).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
HSPH1 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasmic staining in most tissues (HPA tissue IHC) | |
| Staining pattern | Cytoplasmic staining in glandular and neuronal cells (HPA tissue IHC) | |
| Antigen retrieval | EDTA pH 8.0 HIER, heat-mediated (datasheet M04168) | |
| Positive control | Appendix+4 more · see all | |
| Negative control | Adipose tissue+4 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image M04168) | |
| Caveat | Staining has very low concordance with RNA data (HPA tissue IHC) | |
| Regulation | Overexpressed in cancer cells (UniProt) | |
| Isoform / epitope | 4 isoforms; epitope coverage is unknown (UniProt; datasheets) |
Section 1
Recommended HSPH1 IHC & IF Protocols
The catalog antibody protocol uses EDTA pH 8.0 retrieval (datasheet M04168). Three published HSPH1 IHC protocols cover esophageal, liver, and head and neck tissues (PMC13339864; PMC12375714; PMC6139602).
| Sample | Paraffin-embedded human breast cancer tissue; fixative not specified (datasheet M04168) |
| Fixation | Image fixative and duration unreported (datasheet M04168); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: EDTA pH 8.0 (datasheet M04168); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet M04168) |
| Primary antibody | Rabbit monoclonal (clone IOG-8) anti-HSPH1, 1:50 (datasheet M04168) |
| Primary incubation | Overnight at 4 °C (datasheet M04168) |
| Detection | HRP-conjugated secondary, DAB chromogen (datasheet M04168) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | HSPH1-positive staining in glandular cells of appendix (HPA tissue IHC: High). HPA tissue profile: Cytoplasmic expression in most tissues. No signal in the no-primary control. |
💡Decision noteTry heat-mediated EDTA pH 8.0 first for the catalog antibody (datasheet M04168); the published protocols used EDTA pH 9.0 or citrate pH 6.0 (PMC13339864; PMC12375714; PMC6139602).
Section 2
What Is the Expected HSPH1 Staining Pattern?
HSPH1 should appear mainly in the cytoplasm, with high staining reported in selected glandular, respiratory epithelial and neuronal cells (HPA: tissue IHC; UniProt Q92598: cytoplasm). It has no transmembrane segment (UniProt Q92598: topology). Treat tissue intensity as provisional: HPA rates its tissue IHC profile Uncertain because antibody staining and RNA expression show very low consistency (HPA: tissue IHC reliability).
| Cytoplasmic staining is strong in appendix or colon glandular cells, bronchial respiratory epithelial cells, or neurons in caudate or cerebral cortex (HPA: High in these cells). | This matches the reported compartment and selected high-staining cell populations (HPA: tissue IHC; UniProt Q92598: cytoplasm). Compare cell-level staining with a matched negative control; HPA's Uncertain IHC rating limits how confidently intensity alone identifies HSPH1 (HPA: tissue IHC reliability; general IHC practice). |
| Staining is confined to nuclei, without convincing cytoplasmic signal (HPA: cytoplasmic tissue profile). | A nuclear-only IHC result conflicts with the expected predominant cytoplasmic pattern (HPA: tissue IHC; UniProt Q92598: cytoplasm). Limited nucleoplasmic signal is possible in ICC-IF (HPA: additional nucleoplasm), so assess the staining distribution and controls before calling a nuclear signal artefactual (general IHC practice). |
| Adipocytes, cardiomyocytes or skeletal myocytes stain strongly while the expected cytoplasmic positive cells do not (HPA: Not detected in these cell types). | This reverses HPA's reported cell-type contrast and raises concern about cross-reactivity or endogenous chromogenic activity (HPA: tissue IHC; general IHC practice). HPA's Uncertain reliability means a single discordant specimen cannot establish true absence or presence (HPA: tissue IHC reliability). |
| Diffuse colour covers tissue and blank spaces, obscuring cell boundaries (general IHC practice). | This distribution is hard to score as cellular HSPH1 staining; nonspecific reagent binding or excess chromogen development may contribute (general IHC practice). Examine the negative control and identify whether colour follows cells, tissue edges or the whole section (general IHC practice). |
| No cytoplasmic signal appears in glandular cells of appendix or colon, or bronchial respiratory epithelial cells (HPA: High in these cells). | A technical loss of signal is possible, but HPA's tissue IHC profile is Uncertain (HPA: tissue IHC reliability). Confirm tissue preservation, the antibody's IHC-P instructions, detection reagents and a suitable control before interpreting a blank section as HSPH1 absence (general IHC practice). |
💡Expected HSPH1 appearanceCall a result provisionally positive when selected glandular, respiratory epithelial or neuronal cells show predominantly cytoplasmic, potentially strong staining (HPA: High in these cells; HPA: cytoplasmic tissue profile); nuclear-only or widespread acellular colour warrants review (HPA: subcellular ICC-IF; general IHC practice).
| Tissue and cell population (HPA: tissue IHC) | HPA reports High staining in selected glandular, respiratory epithelial and neuronal cells, but Not detected in adipocytes, cardiomyocytes and skeletal myocytes (HPA: tissue IHC). Interpret these as reported contrasts, subject to HPA's Uncertain IHC reliability (HPA: tissue IHC reliability). |
| Antibody evidence (HPA: antibody validation) | The two HPA antibodies with an IHC status are both rated Uncertain, while the ICC-IF ratings are Enhanced or Approved (HPA: HPA028675, CAB002060, HPA031569). ICC-IF validation does not resolve the uncertainty of tissue IHC (HPA: antibody validation). |
| Compartment and processing (UniProt Q92598) | HSPH1 is cytoplasmic, has no transmembrane segment and has no signal peptide or propeptide annotation (UniProt Q92598: subcellular location, topology, processing). An isolated membrane rim or secreted-looking deposit would therefore need control-based review (general IHC practice). |
| Isoforms and antibody recognition (UniProt Q92598) | Four isoforms are listed—Alpha, Beta, 3 and 4 (UniProt Q92598: isoforms). The payload gives no antibody epitope, so it cannot establish which isoforms the catalog antibody detects; avoid attributing staining differences to a particular isoform (UniProt Q92598: isoforms; supplied antibody data). |
| IF/ICC interpretation (HPA: subcellular ICC-IF) | For the separate IF/ICC guide, HPA reports mainly cytosolic signal with additional nucleoplasmic localisation (HPA: subcellular ICC-IF). This supports checking both compartments in cell images, without treating the IF pattern as proof of tissue IHC specificity (HPA: antibody validation). |
| Situation | Likely cause | Next action |
|---|---|---|
| The presumed positive tissue is blank (HPA: High in selected cells). | The specimen or IHC workflow may have failed, or the selected field may lack the reported positive cell population (general IHC practice; HPA: tissue IHC). | Locate the named cell population, inspect morphology and controls, then check the catalog antibody's IHC-P instructions and detection steps (general IHC practice). Keep HPA's Uncertain tissue rating in mind (HPA: tissue IHC reliability). |
| Nuclei stain more strongly than cytoplasm in tissue (HPA: cytoplasmic tissue profile). | The pattern is discordant with predominant cytoplasmic HSPH1 in tissue; ICC-IF nevertheless reports additional nucleoplasmic localisation (HPA: tissue IHC; HPA: subcellular ICC-IF). | Review whether cytoplasm is also stained, compare a negative control and repeat assessment in a reported positive cell population before assigning localisation (general IHC practice; HPA: tissue IHC). |
| Cells reported as Not detected stain strongly (HPA: adipocytes, cardiomyocytes or skeletal myocytes). | Cross-reactivity or endogenous detection activity may mimic target staining (general IHC practice); HPA's tissue rating remains Uncertain (HPA: tissue IHC reliability). | Compare matched negative controls and the cell-by-cell pattern in reported High and Not detected populations; avoid calling the unexpected signal HSPH1 from intensity alone (general IHC practice; HPA: tissue IHC). |
| Diffuse colour obscures cells (general IHC practice). | Nonspecific binding, endogenous detection activity or overdevelopment can raise background in chromogenic IHC (general IHC practice). | Check the negative control and reagent blank as appropriate; review blocking, washes and chromogen development using the existing IHC-P workflow (general IHC practice). |
| A sharp membrane rim dominates (UniProt Q92598: no transmembrane segment). | A membrane-dominant pattern does not fit the annotated cytoplasmic localisation or topology (UniProt Q92598: subcellular location, topology). | Inspect cell boundaries and controls, then compare the same compartment in a reported High-staining tissue; treat persistent rim-only staining as unresolved (general IHC practice; HPA: tissue IHC). |
| Signal differs between specimens or IHC and IF/ICC (HPA: tissue IHC reliability; subcellular ICC-IF). | HPA reports very low consistency between tissue antibody staining and RNA data, while its ICC-IF localisation evidence has separate antibody ratings (HPA: tissue IHC reliability; antibody validation). | Record the cell type, compartment, controls and assay used; assess each application against its own evidence rather than transferring an ICC-IF validation rating to IHC (general IHC practice; HPA: antibody validation). |
Experiment evidence
IHC & IF images showing HSPH1 staining
M04168 · IHCIHC analysis of HSPH1 using anti-HSPH1 antibody (M04168). HSPH1 was detected in a paraffin-embedded section of human breast cancer tissue. H
M04168 · IHCIHC analysis of HSPH1 using anti-HSPH1 antibody (M04168). HSPH1 was detected in a paraffin-embedded section of human stomach cancer tissue.
M04168-1 · IHCIHC analysis of HSPH1 using anti-HSPH1 antibody (M04168-1). HSPH1 was detected in paraffin-embedded section of human lung cancer tissues. He
M04168-1 · IHCIHC analysis of HSPH1 using anti-HSPH1 antibody (M04168-1). HSPH1 was detected in paraffin-embedded section of human mammary cancer tissues.
M04168-1 · IFIF analysis of HSPH1 using anti-HSPH1 antibody (M04168-1). HSPH1 was detected in immunocytochemical section of A431 cell. Enzyme antigen ret
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000120694-HSPH1/tissue/cerebral+cortex#imid_891632
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000120694-HSPH1/tissue/colon#imid_891558
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000120694-HSPH1/tissue/liver#imid_891559
HPA · KidneyHuman Protein Atlas IHC, Kidney. Source: https://www.proteinatlas.org/ENSG00000120694-HSPH1/tissue/kidney#imid_891568
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody CAB002060; green = HSPH1, blue = nucleus, red = microtubules.
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody CAB002060; green = HSPH1, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for HSPH1 IHC & IF
🧪Run appendix first and score its glandular cells, which HPA rates High for HSPH1 (HPA: High in appendix glandular cells). Use adipose tissue as the negative comparator, focusing on adipocytes (HPA: Not detected in adipocytes); on the appendix slide, treat adjacent non-glandular cells as an internal background reference only if they show no specific cellular staining, since HPA does not designate them negative.
Positive control tissue: Appendix (Glandular cells, HPA High)
Negative control tissue: Adipose tissue (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show HSPH1 in A-431, U-251MG, U2OS, U2OS, siRNA 2 (10x), U2OS, siRNA 2 (40x), U2OS, scrambled (10x), U2OS, scrambled (40x), NIH 3T3, with annotated localisation: Cytosol (enhanced) (HPA subcellular).
Technical controls: Include no-primary (secondary-only) and concentration-matched rabbit IgG controls, matching the antibody’s clonality where known (selected-SKU caption: rabbit primary antibody), plus HSPH1 knockout material as a biological negative. Quench endogenous peroxidase and check for background DAB signal in the appendix section before scoring (selected-SKU caption: HRP/DAB detection).
⚠️Feasibility: A target-specific fixation window or fixation effect is unreported, and the selected-SKU paraffin-section caption does not state the fixative (selected-SKU caption: fixative unreported). The caption uses heat retrieval in EDTA at pH 8.0, but does not establish that retrieval is required or compare frozen sections with paraffin sections (selected-SKU caption: EDTA retrieval). Whether frozen sections or IF are easier is unreported; exclude luminal or fold-associated DAB deposits from cellular scoring in appendix glands (HPA: appendix glandular cells; selected-SKU caption: DAB detection).
HPA tissue IHC evidence for HSPH1
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Uncertain — Very low consistency between antibody staining and RNA expression data. Pending external verification. Significant inter-individual differences was observed between vagina RNA expression values.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | Glandular cells | High | Protein (IHC) | HPA → |
| Bronchus | Respiratory epithelial cells | High | Protein (IHC) | HPA → |
| Caudate | Neuronal cells | High | Protein (IHC) | HPA → |
| Cerebellum | Purkinje cells | High | Protein (IHC) | HPA → |
| Cerebral cortex | Neuronal cells | High | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | Adipocytes | Not detected | Protein (IHC) | HPA → |
| Heart muscle | Cardiomyocytes | Not detected | Protein (IHC) | HPA → |
| Ovary | Ovarian stroma cells | Not detected | Protein (IHC) | HPA → |
| Skeletal muscle | Myocytes | Not detected | Protein (IHC) | HPA → |
| Smooth muscle | Smooth muscle cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced HSPH1 IHC Tips
Troubleshoot HSPH1 staining in paraffin sections by checking retrieval, cellular location and controls before comparing signal across specimens.
Which retrieval condition should I start with for HSPH1 in paraffin sections?
Start with heat-mediated antigen retrieval in EDTA at pH 8.0 (datasheet M04168). The selected paraffin-section example used this retrieval before an overnight incubation at 4°C with antibody diluted 1:50 (datasheet M04168). If cytoplasmic staining is weak, compare a slightly longer heating period with the original condition on adjacent sections, while keeping detection and antibody dilution fixed (standard IHC practice). Record heating time and section integrity for each condition, because stronger signal accompanied by tissue damage does not establish better antigen detection (standard IHC practice).
Could fixation explain weak HSPH1 staining in my paraffin sections?
Target-specific fixation sensitivity is unknown: the selected HSPH1 paraffin-section caption does not report a fixative (datasheet M04168). Document the fixative, fixation duration and processing history for each specimen before attributing a weak result to the antibody (standard IHC practice). If comparable sections are available, stain them in the same run using the documented EDTA pH 8.0 retrieval and 1:50 antibody dilution (datasheet M04168; standard IHC practice). Compare cytoplasmic signal and tissue preservation, but treat any difference as a specimen-processing observation until fixation is tested under controlled conditions (UniProt Q92598 subcellular location; standard IHC practice).
How should I assess nuclear HSPH1 staining when cytoplasmic staining is expected?
Use cytoplasmic staining as the principal location check in paraffin sections (UniProt Q92598 subcellular location; HPA tissue IHC profile). Additional nucleoplasmic signal is plausible, although the subcellular observation comes from ICC/IF rather than this tissue-IHC example (HPA subcellular). Compare nuclear and cytoplasmic staining within the same cell population, and examine a counterstained section to distinguish nuclei from adjacent cytoplasm (standard IHC practice). If staining is predominantly nuclear, repeat the run with a no-primary control and review retrieval and chromogen exposure before interpreting it as an HSPH1 distribution change (standard IHC practice).
Can this antibody distinguish HSPH1 isoforms or modified epitopes in tissue?
HSPH1 has 4 annotated isoforms—Alpha, Beta, 3 and 4—but the supplied tissue-IHC caption does not specify an epitope or establish isoform selectivity (UniProt Q92598 isoforms; datasheet M04168). The record also lists acetylated and phosphorylated residues, including phosphoserines at 509 and 510 (UniProt Q92598 modified residues). Do not assign staining differences to one isoform or modification without epitope mapping or an independently validated assay (standard IHC practice). When comparing sections, keep retrieval and detection conditions constant and report the result as total antibody-detected HSPH1 signal unless specificity evidence supports a narrower label (standard IHC practice).
How can I follow up an ambiguous IHC pattern with IF?
For a separate IF experiment, pair HSPH1 with a marker that identifies the cell population being assessed, such as glandular cells in appendix, where tissue IHC reports high signal (HPA tissue IHC; standard IF practice). Choose a red or far-red fluorophore after checking the specimen's autofluorescence and image single-stain controls to assess channel bleed-through (standard IF practice). Use permeabilisation sufficient to expose cytosolic and possible nucleoplasmic epitopes; HSPH1 has no annotated transmembrane segment (HPA subcellular; UniProt Q92598 topology; standard IF practice). Compare the resulting cell-level pattern with paraffin-section staining without assuming that IF conditions transfer to chromogenic IHC (standard IHC/IF practice).
What should I check when HSPH1 DAB staining looks diffuse?
First compare the stained section with a no-primary control to identify signal from secondary detection or endogenous peroxidase (standard IHC practice). The selected example used 10% goat serum blocking, a peroxidase-conjugated secondary antibody and DAB chromogen; its paraffin-section caption does not state the fixative (datasheet M04168). If background persists, check the peroxidase-blocking step, washes and DAB development time on adjacent sections while keeping the EDTA pH 8.0 retrieval constant (standard IHC practice; datasheet M04168). Interpret diffuse brown deposits cautiously when they do not resolve into the predominantly cytoplasmic pattern reported for HSPH1 (HPA tissue IHC profile; standard IHC practice).
How should I quantify HSPH1 staining across paraffin sections? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell population and tissue region before scoring, then record cytoplasmic intensity and the percentage of positive cells to calculate an H-score (HPA tissue IHC profile; standard IHC practice). For spatial comparisons, count positive cells per mm² of evaluable tissue and document excluded areas such as necrosis or section folds (standard IHC practice). Normalise comparisons to the same cell type, area definition, retrieval, antibody dilution and DAB development conditions across specimens (standard IHC practice). Report cell counts and staining distribution alongside the score, because HPA describes cytoplasmic expression in most tissues but rates its tissue-IHC reliability as uncertain (HPA tissue IHC).
How can I distinguish convincing HSPH1 staining from tissue artefact?
A convincing result shows a reproducible cellular pattern centred on the cytoplasm, with any nucleoplasmic signal assessed separately (UniProt Q92598 subcellular location; HPA subcellular; standard IHC practice). Check whether stained cells match the population being scored: HPA reports high staining in appendix glandular cells, while its overall tissue-IHC reliability is uncertain (HPA tissue IHC). Treat staining confined to section edges or necrotic areas as suspect, and use a no-primary control to assess endogenous enzyme or detection-system signal (standard IHC practice). Confirm disputed patterns across sections and document the cell type before interpreting intensity as a biological change (standard IHC practice).
Boster reagents
Best HSPH1 / Heat shock protein 105 kDa IHC Antibodies
HSPH1 antibodies have IHC images from human cancer tissues and rat brain, plus IF/ICC images from A431 and MCF7 cells (catalog IHC/IF captions).
Real IHC data
Anti-Hsp105 Rabbit Monoclonal Antibody
Cat # M04168
Real IHC data
Anti-Hsp105/HSPH1 Antibody ® (monoclonal, 3D10)
Cat # M04168-1
M04168 renders with human breast cancer paraffin-section IHC; additional images show human stomach cancer, stomach tissue and rat brain (M04168 IHC captions). M04168-1 renders with human lung cancer paraffin-section IHC; additional images show human mammary and intestinal cancer IHC and IF/ICC in A431 and MCF7 cells (M04168-1 IHC/IF captions).
Which to pick: For tissue IHC, M04168 has a human breast cancer paraffin-section example using EDTA at pH 8.0 and 1:50 primary, while M04168-1 has a human lung cancer paraffin-section example using citrate at pH 6 and 1 μg/ml primary; fixation is unreported in both captions (M04168 and M04168-1 IHC captions). For IF/ICC, choose M04168-1, a mouse monoclonal with IF images in A431 and MCF7 cells (catalog host/clone; M04168-1 IF captions). For cross-species IHC, choose M04168, a rabbit monoclonal listed as reactive with Human, Mouse and Rat and shown in rat brain paraffin sections; the payload provides no Mouse IHC image for it (catalog host/clone/reactivity; M04168 IHC captions).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry Q92598 (HS105_HUMAN, Heat shock protein 105 kDa).
- Human Protein Atlas. HSPH1 tissue IHC expression (reliability: Uncertain).
- Human Protein Atlas. HSPH1 subcellular location (ICC-IF): Mainly localized to the cytosol. In addition localized to the nucleoplasm..
- Human Protein Atlas. HSPH1 antibody validation summary (3 antibodies).
- Single-cell and bulk omics uncover fibroblast heterogeneity and HSPH1 as a key driver in Barrett's esophagus to esophageal adenocarcinoma progression. Cancer cell international 2026 — PMC13339864.
- Heat shock protein family H member 1 HSPH1 expression correlates with progression and prognosis of hepatocellular carcinoma. Scientific reports 2025 — PMC12375714.
- Correlation of heat shock protein 1 expression with progression and prognosis of non-small cell lung cancer. Frontiers in oncology 2025 — PMC12088961.
- Identification of potential biomarkers of head and neck squamous cell carcinoma using iTRAQ based quantitative proteomic approach. Data in brief 2018 — PMC6139602.
- PubMed PMID:9931472 — UniProt-cited evidence.
- PubMed PMID:9610721 — UniProt-cited evidence.
- PubMed PMID:9039502 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for HSPH1 (Heat shock protein 105 kDa). Expected localisation Cytoplasmic staining in most tissues (HPA tissue IHC). Antigen retrieval: EDTA pH 8.0 HIER, heat-mediated (datasheet M04168). Validated IHC antibody: M04168 at 1:50 (the product's own IHC validation image is shown). Controls (HPA): positive Appendix (Glandular cells); negative Adipose tissue. IHC-P workflow on paraffin sections; fixative unspecified. Protocol steps: 1. Sample: Paraffin-embedded human breast cancer tissue; fixative not specified (datasheet M04168); 2. Fixation: Image fixative and duration unreported (datasheet M04168); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: EDTA pH 8.0 (datasheet M04168); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet M04168); 8. Primary antibody: Rabbit monoclonal (clone IOG-8) anti-HSPH1, 1:50 (datasheet M04168); 9. Primary incubation: Overnight at 4 °C (datasheet M04168). Watch for: Fixation: Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image M04168); Caveat: Staining has very low concordance with RNA data (HPA tissue IHC); Isoform / epitope: 4 isoforms; epitope coverage is unknown (UniProt; datasheets); Regulation: Overexpressed in cancer cells (UniProt). Evidence: UniProt Q92598, HPA tissue IHC · Uncertain, HPA ICC-IF · Mainly localized to the cytosol. In addition localized to the…, Datasheet M04168, PMC13339864, PMC12375714. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/HSPH1/immunohistochemistry-ihc-guide.