This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
ITGAV · Western blot design guide
ITGAV Western Blot Planning Guide
Plan a ITGAV Western blot around the catalog-observed 116 kDa band, image-backed A01561-2 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
ITGAV Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 116 kDa observed | |
| Observed band | 116 kDa observed | |
| Gel | 8-10% | |
| Positive control | Parathyroid gland | |
| Negative control | Duodenum |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 116 kDa |
| ⓘ | Localization | Cell membrane / Cell junction, focal adhesion |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Section 1
Real Curated ITGAV Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Kidney |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A01561-2 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected ITGAV Western Blot Band Size?
Use the product-observed 116 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 116 kDa | Matches the authoritative product WB observation. |
| 116 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected ITGAV appearancePlan around 116 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 116 kDa; use this as the primary experimental expectation. |
| Calculated mass | 116 kDa from UniProt P06756; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A01561-2. |
| Situation | Likely cause | Next action |
|---|---|---|
| 116 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing ITGAV band
A01561-2 · ITGAVObserved 116 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for ITGAV Western blot
🧪Use Parathyroid gland as the first positive-control candidate and retain an orthogonal negative control.
Positive control: Parathyroid gland (High)
Negative control: Duodenum (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for ITGAV
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Parathyroid gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Kidney | Reported tissue cells | High | Protein (HPA) | HPA → |
| Testis | Reported tissue cells | High | Protein (HPA) | HPA → |
| Placenta | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced ITGAV Western Blot Tips
Deeper troubleshooting and optimisation questions for ITGAV, answered from its protein features.
Which band should guide the blot?
Use 116 kDa, the observation attached to the authoritative A01561-2 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 116 kDa expectation.
Which positive control should I start with?
Start with Parathyroid gland, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Duodenum as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for ITGAV Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A01561-2 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
ITGAV Western Blot Reagents
Human-reactive ITGAV Western blot reagents with authoritative product imagery.
Real WB data
Anti-Integrin alpha V/ITGAV Antibody Picoband®
Cat # A01561-2
Real WB data
Anti-Integrin alpha V/ITGAV Antibody Picoband® (monoclonal, 8B10H2)
Cat # M01561-2
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.