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- Table of Contents
ITGAV antibody (A01561-2) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Flow Cytometry analysis of Raji cells using anti-Integrin alpha V/ITGAV antibody (A01561-2).
Overlay histogram showing Raji cells stained with A01561-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin alpha V/ITGAV Antibody (A01561-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Raji cells stained with A01561-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin alpha V/ITGAV Antibody (A01561-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing a Western blot for ITGAV? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the ITGAV Western Blot GuideBundle Save
Anti-Integrin alpha V/ITGAV Antibody Picoband®
ITGAV antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
ITGAV antibody was positively detected by WB in:
human A549 whole cell, human A431 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ITGAV antibody was positively detected by FC in:
Raji cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Predicted
Predicted
Rabbit
Predicted
Predicted
Dog
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Product Information
Product Name
Anti-Integrin alpha V/ITGAV Antibody Picoband®
View all ITGAV Antibodies
View all ITGAV Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Integrin alpha V/ITGAV recombinant protein (Position: H732-D970).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
130-140 kDa
Calculated Molecular Weight
116.0 kDa
Background of ITGAV
Integrin alpha-V is a protein that in humans is encoded by the ITGAV gene. It is a member of the beta 3 integrin subfamily (cytoadhesins). The human locus for the av gene (VNRA) was previously mapped to the long arm of chromosome 2. Sims et al. (2000) localized the VNRA gene to 2q31. The gene contains 30 exons and spans over 93 kb of genomic DNA. It functions as a receptor for a group of proteins that includes vitronectin, fibrinogen, thrombospondin, and von Willebrand factor.
Cite This Product
Anti-Integrin alpha V/ITGAV Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01561-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Rabbit
93%
High Homology
Dog
93%
High Homology
Cow
93%
High Homology
Sheep
92%
High Homology
Mouse
89%
Moderate Homology
Rat
89%
Moderate Homology
Zebrafish
56%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ITGAV antibody
Flow Cytometry (FC) protocol for ITGAV antibody
Publications
Application:
Species:
TGFβ3-mediated induction of Periostin facilitates head and neck cancer growth and is associated with metastasis
Published: 2016-02-09
Journal: Scientific Reports
Application: IA
Impact Factor: 5.23
Species: Human
PMID: 26857387
Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors
Published: 2011-05-27
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: IHC,IF
Impact Factor: 2.28
Species: Human
PMID: 21747684
Overexpression of Lewis (y) Antigen Protects Ovarian Cancer RMG-1 Cells from Carboplatin-Induced Apoptosis by the Upregulation of Topo-I and Topo-II β
Published: 2011-05-03
Journal: Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
Application: WB,FC
Impact Factor: 1.40
Species: Human
PMID: 21542140
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Product Promise
We promise Anti-Integrin alpha V/ITGAV Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01561-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 26, 2026