This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about Junctional adhesion molecule C.
Functions as a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) (PubMed:12208882, PubMed:15194813). Plays a role in angiogenesis (PubMed:23255084).
Human | |
---|---|
Gene Name: | JAM3 |
Uniprot: | Q9BX67 |
Entrez: | 83700 |
Belongs to: |
---|
immunoglobulin superfamily |
CD323; JAM-2; JAM3; JAMC; JAM-C; JAM-CFLJ14529; junctional adhesion molecule 3JAM-3; junctional adhesion molecule C
Mass (kDA):
35.02 kDA
Human | |
---|---|
Location: | 11q25 |
Sequence: | 11; NC_000011.10 (134068925..134152001) |
Detected on round and elongated spermatids (at protein level) (PubMed:15372036). Highest expression in placenta, brain and kidney. Significant expression is detected on platelets. Expressed in intestinal mucosa cells. Expressed in the vascular endothelium. Found in serum (at protein level). Also detected in the synovial fluid of patients with rheumatoid arthritis, psoriatic arthritis or ostearthritis (at protein level).
Cell membrane; Single-pass type I membrane protein. Cell junction. Cell junction, desmosome. Cell junction, tight junction. Detected in the acrosome region in developing spermatids (By similarity). In epithelial cells, it is expressed at desmosomes but not at tight junctions (PubMed:15194813). Localizes at the cell surface of endothelial cells; treatment of endothelial cells with vascular endothelial growth factor stimulates recruitment of JAM3 to cell-cell contacts (PubMed:15994945).; [Soluble form of JAM-C]: Secreted.
This article will cover the various methods you have to calculate the JAM3 marker's concentration. It also explains how to calculate JAML OD values in microtiterplate readers. The standard curve method is used to optimize your JAML test. Once you have your data, you can perform a quantitative study to determine the concentrations of JAML in your samples.
The JAM3 marker is associated to adherent microparticles and platelets. The JAM3 test is shorter and safer than the standard (51)Cr-release assay. In addition, it is less invasive and safer to perform than the current standard assay. But how to optimize your JAML assay with the JAM3 marker?
There are a few steps that can be used to calculate JAML concentration via OD. ODs are dependent on wavelength and relate concentration to attenuation. If you measure OD at the wavelength where the cells are close together, you can use the Beer-Lambert law. Otherwise, you should consider the distance between the particles. If you need to calculate the OD per photograph, you can do so as well. The wavelength you use for OD measurement should be more accurate than the one used for absorption.
A microplate reader is able to measure the optical densities of cultures. The reader's reading of the spectral response measures how much incident light passes through the sample. Darker cultures exhibit lower Transmittance values than lighter ones. Log10T is the ratio OD426 value to the intensity or light incident on a specimen. One OD426 value equals log10T.
Microbiology uses OD measurements frequently. They are often used to determine antibiotic efficacy, characterize mutant strains, and determine their growth rate in different environments. While OD is proportional to cell count, there are many variables that can affect the relationship between OD/cell concentration. To accurately calculate cell concentration using OD measurements it is important to use a calibration procedure.
The OD-C relationship for scatterers with different sizes is expected to differ. The C will be higher in bioreactor experiments than in laboratory tests. Cell size can change throughout growth. Cell size can also be affected by growth under antibiotics, strains that are overexpressed, and chains or clusters. In such cases, it is better to use microscopy to determine the OD of a culture instead of OD measurements.
Light scattering is a common problem when performing biochemical analyses. There are solutions to single scattering situations, such the Beer-Lambert Law. The Beer-Lambert Law is not applicable to multi-scattering situations. There are various approximations, which are based on r and l and the wavelength of light.
The simplest and most common spectrophotometer uses small tubes or cuvettes made of plastic that contain bacterial cultures. Cuvettes should be thick as this will allow the laser travel farther and give you more accurate readings. These cuvettes do NOT have lids. The sample used to measure OD600 is not allowed to be added to the culture.
The OD can determine the growth rate and lag time of bacteria cells as well as the cell yield. This method is simple to use and doesn't require expensive equipment. Microtiter plate reader can be used to measure many samples, such yeast, bacteria, and mushrooms. When used correctly, they enable researchers to analyze microbial growth, lag, and cell yield.
PMID: 11590146 by Arrate M.P., et al. Cloning of human junctional adhesion molecule 3 (JAM3) and its identification as the JAM2 counter-receptor.
PMID: 11739175 by Aurrand-Lions M.A., et al. Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members.