Mouse JAM-C/JAM3 ELISA Kit PicoKine®

JAM-C ELISA kit for Mouse

Mouse JAM-C/JAM3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Jam3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1840
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Mouse JAM-C/JAM3 ELISA Kit PicoKine®

View all JAM-C ELISA kits

SKU/Catalog Number

EK1840

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse JAM-C/JAM3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Jam3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse JAM-C/JAM3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1840)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E30-N241

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1840 is reactive to Jam3 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1840 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of JAM-C

JAM3 belongs to the junctional adhesion molecule protein family and functions as a receptor for another member of this family. This gene is mapped to 9; 9 A4. JAM3 plays a part in cell-cell adhesion. The soluble form of JAM3 is a mediator of angiogenesis. JAM3 is a counter-receptor for ITGAM, mediating leukocyteplatelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). A mutation in an intron of the JAM3 gene is linked with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts.

Kit Components

Catalog Number Description Quantity
EK1840-CAP Anti-Mouse Jam3 Pre-coated 96-well strip microplate 1
EK1840-ST Mouse Jam3 Standard 2 vials, 10 ng/tube
EK1840-DA Mouse Jam3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.2562.512525050010002000
O.D.0.0070.0700.1830.3140.4900.8961.5122.129

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect JAM-C, Dilution ratio of 1:1, concentration in serum is around 90pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse JAM-C/JAM3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5827088461279991
Standard deviation2.9014.0439.782.8117.8674.33
CV (%)5.0%5.2%4.5%4.6%6.4%7.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of JAM-C in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 158545549543.246.0%
Sample 227032029129729417.816.0%
Sample 388481886693087440.104.5%
*number of samples for each test n=16.

Gene/Protein Information For Jam3 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9D8B7

Gene ID

83964

Gene Name

Jam3

Full Name

Junctional adhesion molecule C

Weight

34.838kDa

Superfamily

immunoglobulin superfamily

Alternative Names

CD323; JAM-2; JAM3; JAMC; JAM-C; JAM-CFLJ14529; junctional adhesion molecule 3JAM-3; junctional adhesion molecule C Jam3|1110002N23Rik, JAM-, JAM-3, JAM-C, Jcam, Jcam3|junction adhesion molecule 3|junctional adhesion molecule C|junction cell adhesion molecule 3|junction cell adhesion molecule C|junctional adhesion molecule 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Jam3, check out the Jam3 Infographic

Jam3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Jam3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Mouse JAM-C/JAM3 ELISA Kit PicoKine®

Question

Q: how do I analyze ELISA data? I have performed JAM-C level in plasma.

Verified Customer

Verified customer

Asked: 2020-11-22

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-11-22

Question

Q: how can I thaw whole blood sample for JAM-C ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-10-25

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test JAM-C for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-10-25

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-07-16

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-07-16

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification JAM-C using the EK1840 Mouse JAM-C Picokine® ELISA Kit?

B. King

Verified customer

Asked: 2018-05-13

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2018-05-13

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of JAM-C in Mouse plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

C. Kulkarni

Verified customer

Asked: 2018-03-27

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the JAM-C ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-03-27

Question

Q: is it okay to use citrate plasma as samples in Mouse JAM-C Picokine® ELISA Kit (Catalog # EK1840)?

Verified Customer

Verified customer

Asked: 2017-03-24

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of JAM-C causing disruption of its protein structure. JAM-C may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the JAM-C ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2017-03-24

Question

Q: What is the optimal O.D. value for JAM-C ELISA kit? I used your JAM-C ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain JAM-C even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2017-03-07

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this JAM-C ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of JAM-C that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2017-03-07

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