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- Table of Contents
KRT10 / Keratin, type I cytoskeletal 10 · Western blot design guide
Design a Western Blot for KRT10
Real validated KRT10 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-KRT10 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
KRT10 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~58.8 kDa | |
| Observed band | ~59 kDa | |
| Gel | 5–20% (catalog M02305-1) | |
| Positive control | Skin (IHC candidate; verify WB) | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Real Curated KRT10 Western Blot Protocols
The M02305-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human K562, human SH-SY5Y, human Jurkat (catalog M02305-1) |
| Gel % | 5–20% (catalog M02305-1) |
| Load | 30 ug; reducing conditions (catalog M02305-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog M02305-1) |
| Membrane | nitrocellulose membrane (catalog M02305-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog M02305-1) |
| Primary antibody | M02305-1 · 1:500 (catalog M02305-1) |
| Primary incubation | overnight at 4°C (catalog M02305-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:500 (catalog M02305-1) |
| Secondary incubation | 1.5 hour at RT (catalog M02305-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog M02305-1) |
| Detection | ECL (catalog M02305-1) |
Section 2
What Is the Expected KRT10 Western Blot Band Size?
KRT10 is predicted at 58.8 kDa and observed at ~59 kDa; the small difference has no established cause in the supplied evidence.
| Band at ~59 kDa | Matches the observed KRT10 band near its 58.8 kDa predicted mass |
| Higher band under nonreducing conditions | May reflect retention of a Cys401-linked complex |
| Additional higher bands | May reflect KRT10 complexes with KRT1 if they survive sample preparation |
| Little or no band in whole-cell lysate | May reflect KRT10's extracellular localization, although KRT10 is also reported in the cytoplasm |
💡Expected KRT10 appearanceKRT10 has a predicted mass of 58.8 kDa and an observed band at ~59 kDa; confirm band identity with an appropriate positive control or KRT10 depletion.
| Predicted KRT10 mass | 58.8 kDa predicted; the observed band is ~59 kDa |
| Cys401 disulfide | Could retain a higher-mass complex under nonreducing conditions |
| KRT1–KRT10 heterodimer | Could migrate above monomeric KRT10 if retained during electrophoresis |
| KRT1–KRT10 heterotetramer | Could produce higher-mass material if the complex remains intact |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | KRT10 can localize extracellularly, and lysate expression may be low | Check a KRT10-positive sample and compare extracellular material |
| Band higher than expected | A Cys401-linked complex may persist with incomplete reduction | Compare reducing and nonreducing samples |
| Band lower than expected | Its identity is uncertain; no precursor cleavage is listed | Compare with a positive control and test KRT10 depletion |
| Multiple bands | Retained KRT1 complexes or unrelated antibody binding may contribute | Compare reduction conditions and confirm bands by KRT10 depletion |
| Weak or no signal | KRT10 may be scarce in the tested sample | Use a KRT10-positive control and check sample loading |
Experiment evidence
Western blot images showing KRT10 band
M02305-1 · KRT10
A02305-2 · KRT10
A02305-1 · KRT10
A02305-1 · KRT10
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for KRT10 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for KRT10 in Western blot, you can use skin tissue.
Positive control: Skin (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: Skin provides a positive control, but extracellular KRT10 may give variable signal in whole-cell lysate.
HPA tissue expression evidence for KRT10
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Skin | cells in corneal layer | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Appendix | glandular cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced KRT10 Western Blot Tips
Deeper troubleshooting and optimisation questions for KRT10, answered from its protein features.
How should KRT10 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could a second KRT10 isoform explain another band?
Isoforms · Only one isoform is listed, with no alternative sequence. These features do not support assigning another band to a KRT10 isoform.
Which KRT10 phosphorylation sites matter when interpreting bands?
PTM · UniProt lists phosphoserine at positions 14, 16, 42, 53, 56, and 170. Use those UniProt coordinates when comparing antibody or paper numbering. Their presence alone does not establish a visible band shift.
No glycosylation sites are listed. The supplied features therefore do not support attributing a heavier band to glycosylation.
No glycosylation sites are listed. The supplied features therefore do not support attributing a heavier band to glycosylation.
Does this guide establish induction of KRT10?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for KRT10?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M02305-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should KRT10 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
How does the observed KRT10 band compare with predicted mass?
Interpretation · The observed band near 59 kDa closely matches KRT10’s predicted 58.8 kDa. The listed features do not establish a cause for any small difference in apparent mass.
KRT10 forms a heterodimer with KRT1, and two such heterodimers form a heterotetramer. A higher band cannot be identified as one of these complexes from molecular weight alone; interpretation depends on the sample and blot conditions.
KRT10 is listed in the cytoplasm, at the cell surface, and in extracellular space. When quantifying samples, compare like fractions and use the same preparation method; different fractions may contain different amounts of KRT10.
Check the band against the approximately 59 kDa observed band and 58.8 kDa predicted mass, then consider the listed phosphorylation sites and KRT1-containing complexes. One listed disulfide bond may also make reducing conditions relevant. None of these features alone identifies an unexpected band.
KRT10 forms a heterodimer with KRT1, and two such heterodimers form a heterotetramer. A higher band cannot be identified as one of these complexes from molecular weight alone; interpretation depends on the sample and blot conditions.
KRT10 is listed in the cytoplasm, at the cell surface, and in extracellular space. When quantifying samples, compare like fractions and use the same preparation method; different fractions may contain different amounts of KRT10.
Check the band against the approximately 59 kDa observed band and 58.8 kDa predicted mass, then consider the listed phosphorylation sites and KRT1-containing complexes. One listed disulfide bond may also make reducing conditions relevant. None of these features alone identifies an unexpected band.
Boster reagents
KRT10 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Cytokeratin 10 KRT10 Rabbit Monoclonal Antibody
Cat # M02305-1
Real WB data
Anti-Cytokeratin 10 KRT10 Antibody
Cat # A02305-2
Real WB data
Anti-Cytokeratin 10 KRT10 Antibody
Cat # A02305-1
Three the supplier anti-KRT10 antibodies list human, mouse, and rat reactivity and have WB images. Captions describe specific tested samples; the detail varies, and these examples do not establish performance across all tissues or conditions.
Which to pick: Choose M02305-1 for the most detailed WB example: human cell lines and rat and mouse brain lysates, with a reported 59 kDa band. A02305-2 shows HeLa lysate; A02305-1 describes only ‘various cells.’ Match your sample and conditions to the available evidence.
Source: BosterBio KRT10 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P13645.
- Human Protein Atlas. KRT10 tissue expression.
- PMC4013593 — target-verified WB comparison
- PMC5805778 — target-verified WB comparison
- PMC12155225 — target-verified WB comparison