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Product Info Summary
| SKU: | M02305-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytokeratin 10 KRT10 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02305-1
BM4422 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 10 KRT10 Rabbit Monoclonal Antibody catalog # M02305-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 10 KRT10 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02305-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EEF-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytokeratin 10
Reactive Species
M02305-1 is reactive to KRT10 in Human, Mouse, Rat
Observed Molecular Weight
59 kDa
Calculated molecular weight
58.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02305-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: human K562 whole cell, human SH-SY5Y whole cell, human Jurkat whole cell, rat brain tissue, mouse brain tissue
IHC: human bladder tissue
ICC/IF: HACAT cell
Validation Images & Assay Conditions
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Western blot analysis of KRT10 using anti-KRT10 antibody (M02305-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT10 antigen affinity purified monoclonal antibody (Catalog # M02305-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT10 at approximately 59 kDa. The expected band size for KRT10 is at 59 kDa.
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Immunofluorescent analysis of HACAT cells, using Cytokeratin 10 Antibody.
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Immunohistochemical analysis of paraffin-embedded human bladder, using Cytokeratin 10 Antibody.
Specific Publications For Anti-Cytokeratin 10 KRT10 Rabbit Monoclonal Antibody (M02305-1)
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1 Customer Q&As for Anti-Cytokeratin 10 KRT10 Rabbit Monoclonal Antibody
Question
We are currently using anti-Cytokeratin 10 Rabbit Monoclonal antibody M02305-1 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
The anti-Cytokeratin 10 Rabbit Monoclonal antibody (M02305-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-05
