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Product Info Summary
| SKU: | A02305-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Cytokeratin 10 KRT10 Antibody
SKU/Catalog Number
A02305-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 10 KRT10 Antibody catalog # A02305-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 10 KRT10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02305-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Keratin 10. AA range:136-185
Reactive Species
A02305-1 is reactive to KRT10 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
58.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02305-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Cytokeratin 10 Polyclonal Antibody
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Western Blot analysis of HeLa cells using Cytokeratin 10 Polyclonal Antibody
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
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Immunofluorescence analysis of A549 cells, using Keratin 10 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Cytokeratin 10 KRT10 Antibody (A02305-1)
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Customer Q&As
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6 Customer Q&As for Anti-Cytokeratin 10 KRT10 Antibody
Question
Here is the WB image, lot number and protocol we used for skin using anti-Cytokeratin 10 antibody A02305-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-17
Question
I see that the anti-Cytokeratin 10 antibody A02305-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-03-03
Question
Would anti-Cytokeratin 10 antibody A02305-1 work on dog WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
Our lab technicians have not validated anti-Cytokeratin 10 antibody A02305-1 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Cytokeratin 10 antibody A02305-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-27
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-Cytokeratin 10 antibody A02305-1. Let me know if you need anything else.
T. Dhar
Verified customer
Asked: 2018-10-19
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-19
Question
My question regarding product A02305-1, anti-Cytokeratin 10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02305-1 anti-Cytokeratin 10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-09
Question
I was wanting to use your anti-Cytokeratin 10 antibody for WB for mouse skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse skin identification?
Verified Customer
Verified customer
Asked: 2017-11-22
Answer
You can see on the product datasheet, A02305-1 anti-Cytokeratin 10 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-22
