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- Table of Contents
MALT1 / Mucosa-associated lymphoid tissue lymphoma translocation protein 1 · Western blot design guide
Design a Western Blot for MALT1
Real validated MALT1 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-MALT1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
MALT1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~92.3 kDa | |
| Gel | 8–10% (standard starting point) | |
| Positive control | Lymph node (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 2 isoform(s) |
Section 1
Real Curated MALT1 Western Blot Protocols
The A01599 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | EL4 cell lysate (catalog A01599) |
| Gel % | 8–10% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A01599; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected MALT1 Western Blot Band Size?
MALT1 is predicted at 92.3 kDa; isoforms and oligomerization could affect bands, but their migration has not been demonstrated here.
| Band near 92.3 kDa | Consistent with full-length MALT1; confirm identity with antibody controls |
| Additional band at a different size | Could represent isoform 1 or 2; their migration has not been established |
| Higher-mass band | Could reflect a MALT1 oligomer retained during preparation; its migration is not established |
| Bands in nuclear and cytoplasmic fractions | Consistent with MALT1 shuttling between these compartments |
💡Expected MALT1 appearanceUniProt predicts 92.3 kDa for MALT1; no empirical band size is supplied, so verify any band near that size with antibody and fractionation controls.
| UniProt predicted mass | Full-length MALT1 is predicted to be 92.3 kDa |
| Splice isoform 1 | May differ in size from isoform 2; its mass and migration are not supplied |
| Splice isoform 2 | May differ in size from isoform 1; its mass and migration are not supplied |
| MALT1 homooligomerization | Could produce higher-mass species if oligomers persist during sample preparation; no specific oligomer band is established |
| Situation | Likely cause | Next action |
|---|---|---|
| Band higher than expected | A MALT1 oligomer may have persisted during preparation | Compare denaturing conditions and confirm the band with an independent MALT1 antibody |
| Band lower than expected | A splice isoform or proteolytic fragment is possible; its identity is unverified | Check sample handling and confirm identity with antibodies to different MALT1 regions |
| Multiple bands | MALT1 has two annotated splice isoforms, but distinct band sizes are unproven | Compare isoform-specific samples or reagents and verify each band independently |
| Weak or no signal | MALT1 may be distributed between nuclear and cytoplasmic fractions | Check whole-cell lysate and both fractions with fractionation controls |
| Fragments below expected size | Proteolysis during sample preparation is possible | Prepare fresh lysate with protease inhibitors and compare antibodies to different MALT1 regions |
Experiment evidence
Western blot images showing MALT1 band
A01599 · MALT1
M01599-2 · MALT1
M01599-1 · MALT1
PA1920 · MALT1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for MALT1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for MALT1 in Western blot, you can use lymph node lysate, which HPA scores as high for MALT1.
Positive control: Lymph node (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA reports no detection in adipose tissue, but confirm its negative signal by Western blot.
HPA tissue expression evidence for MALT1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Lymph node | germinal center cells | High | Protein (IHC) | HPA → |
| Tonsil | germinal center cells | High | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Medium | Protein (IHC) | HPA → |
| Endometrium | glandular cells | Medium | Protein (IHC) | HPA → |
| Stomach | glandular cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | Not detected | Protein (IHC) | HPA → |
| Caudate | glial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced MALT1 Western Blot Tips
Deeper troubleshooting and optimisation questions for MALT1, answered from its protein features.
How should MALT1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could MALT1 isoforms produce different bands?
Isoforms · Two isoforms are listed. Isoform 2 lacks residues 309–319 of isoform 1, so check whether the antibody epitope includes that region when interpreting isoform detection. The record does not establish whether the isoforms resolve as separate bands.
Which MALT1 modifications matter when interpreting bands?
PTM · UniProt lists N-acetylserine at position 2 and phosphoserine at position 135. These are UniProt coordinates; antibody or paper numbering may differ. Neither modification alone establishes a detectable mobility shift.
Does this guide establish induction of MALT1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for MALT1?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A01599 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should MALT1 be quantified across samples?
Quantitation · Compare the same fraction across samples and quantify the band consistently, accounting for MALT1's nuclear and cytoplasmic distribution. If more than one band appears, define which band or bands are included before comparing signals.
How should the predicted MALT1 mass guide band identification?
Interpretation · The supplied predicted mass is 92.3 kDa, but no observed band position is available. Use 92.3 kDa as a reference, not a guaranteed migration position; the listed features do not establish a visible shift.
MALT1 is listed in the cytoplasm, perinuclear region, and nucleus, and shuttles between nucleus and cytoplasm. If comparing fractions, account for this distribution before interpreting a weak or absent band in one fraction.
Check antibody recognition of the two isoforms, including isoform 2's missing residues 309–319. MALT1 also forms homooligomers, but the supplied features do not show that these survive Western blot preparation or explain a particular band.
MALT1 forms homooligomers that bind TRAF6 and participates in a CARD14–BCL10–MALT1 complex. These interactions give context for unexpected bands, but the record does not establish their migration under your blot conditions.
MALT1 is listed in the cytoplasm, perinuclear region, and nucleus, and shuttles between nucleus and cytoplasm. If comparing fractions, account for this distribution before interpreting a weak or absent band in one fraction.
Check antibody recognition of the two isoforms, including isoform 2's missing residues 309–319. MALT1 also forms homooligomers, but the supplied features do not show that these survive Western blot preparation or explain a particular band.
MALT1 forms homooligomers that bind TRAF6 and participates in a CARD14–BCL10–MALT1 complex. These interactions give context for unexpected bands, but the record does not establish their migration under your blot conditions.
Boster reagents
MALT1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-MALT1 Antibody
Cat # A01599
Real WB data
Anti-MALT1 Rabbit Monoclonal Antibody
Cat # M01599-2
Real WB data
Anti-MALT1 Monoclonal Antibody
Cat # M01599-1
Real WB data
Anti-MALT1 Antibody Picoband®
Cat # PA1920
All four listed anti-MALT1 antibodies have Western blot images. Their captions document specific cell lysates and conditions; PA1920 includes human, rat, and mouse samples. These examples do not establish performance across every sample of a listed species.
Which to pick: For human lysates, choose by the closest illustrated sample: M01599-1 shows five human cell lines, M01599-2 shows K562, and PA1920 shows 293T, Jurkat, and Raji. For rat or mouse lysates, PA1920 illustrates both species; A01599 illustrates EL4.
Source: BosterBio MALT1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q9UDY8.
- Human Protein Atlas. MALT1 tissue expression.
- PMC5214165 — target-verified WB comparison
- PMC10093854 — target-verified WB comparison
- PMC4126661 — target-verified WB comparison