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- Table of Contents
MPC1 / Mitochondrial pyruvate carrier 1 · Western blot design guide
Design a Western Blot for MPC1
Real validated MPC1 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-MPC1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
MPC1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~12.3 kDa | |
| Gel | 15% (standard starting point) | |
| Positive control | Appendix (IHC candidate; verify WB) +4 more | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Acetylated | |
| Caveat | Peptide-blocking control | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Real Curated MPC1 Western Blot Protocols
The A30578 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | various cells (catalog A30578) |
| Gel % | 15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.2 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A30578; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected MPC1 Western Blot Band Size?
MPC1 has a predicted monomer mass of 12.3 kDa; dimerization could affect migration, but no empirical band or demonstrated shift is supplied.
| Band near 12.3 kDa | consistent with the predicted MPC1 monomer size; confirm identity with an antibody control |
| Band near 25 kDa | could reflect a retained MPC1 homodimer; dimer formation and migration are unverified |
| Higher band of uncertain size | could reflect retained MPC1–MPC2 association; band identity requires confirmation |
| Weak band in a soluble fraction | MPC1 resides in the mitochondrial inner membrane and may be poorly recovered |
💡Expected MPC1 appearanceMPC1 has a predicted monomer mass of 12.3 kDa, but no empirical band size is supplied; confirm a candidate band with appropriate antibody and sample controls.
| Predicted MPC1 monomer mass | sets a 12.3 kDa sequence-based reference, not a measured migration |
| 109-residue MPC1 sequence | underlies the predicted monomer mass; no cleavage is listed |
| Possible MPC1 homodimer | could produce a band near twice the monomer mass if it remains intact during electrophoresis |
| MPC1–MPC2 heterodimer | could migrate above the MPC1 monomer if retained; its apparent mass is not supplied |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | MPC1 is an inner-membrane protein that may be poorly extracted | check mitochondrial membrane recovery and compare an enriched fraction |
| Band higher than expected | a retained MPC1 dimer is possible | compare reducing and denaturing conditions and verify band identity |
| Band lower than expected | band identity or sample integrity is uncertain | check sample integrity and use an antibody specificity control |
| Multiple bands | retained complexes or antibody cross-reactivity are possible | compare denaturing conditions and use a peptide-block or independent antibody control |
| Weak or no signal | poor recovery of the inner-membrane protein is possible | check membrane extraction, loading, and a positive sample |
Experiment evidence
Western blot images showing MPC1 band
A30578 · MPC1
A30578 · MPC1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for MPC1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for MPC1 in Western blot, you can use appendix tissue, which HPA rates High.
Positive control: Appendix (IHC candidate; verify WB)
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside.
⚠️Feasibility: HPA lists no Not-detected tissue, so use siRNA knockdown or a KO line for a clean negative control.
HPA tissue expression evidence for MPC1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | glandular cells | High | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | High | Protein (IHC) | HPA → |
| Cerebellum | cells in granular layer | High | Protein (IHC) | HPA → |
| Colon | glandular cells | High | Protein (IHC) | HPA → |
| Duodenum | glandular cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Low | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Low | Protein (IHC) | HPA → |
| Lymph node | non-germinal center cells | Low | Protein (IHC) | HPA → |
| Oral mucosa | squamous epithelial cells | Low | Protein (IHC) | HPA → |
| Ovary | ovarian stroma cells | Low | Protein (IHC) | HPA → |
Section 3
Advanced MPC1 Western Blot Tips
Deeper troubleshooting and optimisation questions for MPC1, answered from its protein features.
Where should the MPC1 band appear?
Band shift · MPC1 has a predicted mass of 12.3 kDa. No observed Western-blot band position is supplied, so use 12.3 kDa as a reference, not a confirmed apparent mass.
Could an MPC1 isoform produce another band?
Isoforms · The supplied UniProt features list one isoform and no alternative sequence. They provide no basis for assigning an additional band to an MPC1 isoform.
Do MPC1 modifications explain a band shift?
PTM · UniProt lists N-acetylalanine at position 2 and N6-acetyllysine at position 72. These are UniProt coordinates; paper or antibody numbering may differ. Their presence alone does not establish a visible shift or explain a difference from 12.3 kDa.
Does this guide establish induction of MPC1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for MPC1 Western blot?
Transfer · MPC1 is a small, 12.3 kDa multi-pass inner-mitochondrial-membrane protein. Choose transfer conditions that retain small proteins, and check the post-transfer gel and membrane to assess transfer and retention.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A30578 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should MPC1 signal be quantified?
Quantitation · Because MPC1 is an inner-mitochondrial-membrane protein, compare samples prepared with a consistent membrane-extraction procedure and equal protein input. Measure bands within the detection method's linear range; the supplied features provide no observed band position for defining a quantitation window.
How should unexpected MPC1 bands be interpreted?
Interpretation · MPC1 forms a functional heterodimer with MPC2 and may form a homodimer, but these features do not establish that either complex survives Western-blot preparation. The supplied features do not identify an observed band, so avoid assigning unexpected bands to complexes or modifications without further evidence.
Boster reagents
MPC1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-BRP44L Antibody
Cat # A30578
the supplier A30578 (Anti-BRP44L) is listed for MPC1 with reported human, mouse, and rat reactivity and a Western blot image described as using various cells. The supplied evidence does not establish independent validation.
Which to pick: A30578 is the only listed option. Check its reported reactivity against your sample species; its WB image offers an example, though the supplied caption does not identify the individual cell samples or conditions.
Source: BosterBio MPC1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q9Y5U8.
- Human Protein Atlas. MPC1 tissue expression.
- PMC13380138 — target-verified WB comparison
- PMC5958719 — target-verified WB comparison
- PMC4866774 — target-verified WB comparison