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Product Info Summary
| SKU: | A30578 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BRP44L Antibody
SKU/Catalog Number
A30578
Size
100ul
Form
Liquid
Description
Boster Bio Anti-BRP44L Antibody (Catalog# A30578). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BRP44L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30578)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human BRP44L. Immunogen sequence location: 11-60
Reactive Species
A30578 is reactive to MPC1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
12.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30578 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of Hela cell. 1,Brp44L Polyclonal Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min).
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Western Blot analysis of various cells using Brp44L Polyclonal Antibody
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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using BRP44L Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COLO and HeLa cells, using BRP44L Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-BRP44L Antibody (A30578)
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1 Customer Q&As for Anti-BRP44L Antibody
Question
We are currently using anti-BRP44L antibody A30578 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
The anti-BRP44L antibody (A30578) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-24
