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- Table of Contents
NEUROG2 / Neurogenin-2 · Western blot design guide
Design a Western Blot for NEUROG2
Source-linked NEUROG2 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-NEUROG2 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
NEUROG2 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~28.6 kDa | |
| Gel | 12–15% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked NEUROG2 Western Blot Protocol Options
The A10153 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | mouse small intestine tissue lysate (catalog A10153) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A10153 · (A) 1 and (B) 2 μg/mL (catalog A10153) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected NEUROG2 Western Blot Band Size?
NEUROG2 is predicted at 28.6 kDa; no empirical band is supplied, and its documented features do not establish a migration shift.
| Single band near 28.6 kDa | Consistent with the predicted NEUROG2 mass; verify identity with controls |
| Band enriched in nuclear fraction | Consistent with NEUROG2 nuclear localization |
| Weaker band in cytoplasmic fraction | Consistent with NEUROG2 nuclear localization |
| Band at the same position in whole-cell and nuclear fractions | Consistent with the same NEUROG2 species in both samples |
💡Expected NEUROG2 appearanceUniProt predicts a 28.6 kDa NEUROG2 band, but no empirical band size is supplied; verify any candidate band with ordinary identity controls.
| UniProt predicted molecular weight | Sets a 28.6 kDa reference for the full-length protein |
| Sequence-based mass | The 272-residue sequence has a calculated mass of 28,621 Da |
| Predicted monomer mass | Suggests a band near 28.6 kDa if the protein migrates as predicted |
| UniProt mass reference | Provides a comparison point; actual gel migration is unverified |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | NEUROG2 may be scarce in the sampled cells | Confirm expression and compare a nuclear-enriched fraction |
| Band higher than expected | No documented size-increasing feature establishes its identity | Check specificity with NEUROG2 depletion or an independent antibody |
| Band lower than expected | No documented cleavage establishes a smaller NEUROG2 product | Check sample integrity and verify identity with an independent antibody |
| Multiple bands | The supplied features do not identify distinct isoform bands | Compare each band with a NEUROG2 depletion control |
| Weak or no signal | NEUROG2 abundance may be low in the sample | Confirm expression and assess a nuclear-enriched sample |
Experiment evidence
Western blot images showing NEUROG2 band
A10153 · NEUROG2
M10153 · NEUROG2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for NEUROG2 Western blot
🧪For positive controls for NEUROG2 in Western blot, you can use an HPA-IHC candidate positive sample once one is identified; none is supplied here.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA provides no tissue data, so tissue-based controls cannot be confirmed.
HPA tissue expression evidence for NEUROG2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced NEUROG2 Western Blot Tips
Deeper troubleshooting and optimisation questions for NEUROG2, answered from its protein features.
Where should the NEUROG2 band appear?
Band shift · The predicted mass is 28.6 kDa for the 272-residue protein. No empirical apparent band position is supplied, so use 28.6 kDa as a reference rather than an exact migration target.
Are multiple NEUROG2 isoform bands expected?
Isoforms · Only one isoform is listed, with no alternative sequence. The supplied features therefore do not support assigning multiple bands to annotated isoforms.
Do annotated modifications prove a band shift?
PTM · The linked UniProt record describes protein features. A modification annotation alone does not demonstrate a visible shift; retain any condition or experimental qualifier attached to it.
Does this guide establish induction of NEUROG2?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for NEUROG2?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A10153 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should NEUROG2 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Does the predicted mass explain a shifted NEUROG2 band?
Interpretation · No observed band position is supplied for comparison. The listed features include no modified residues or glycosylation sites; they do not establish the cause of a band shift.
NEUROG2 is annotated as nuclear. Include a nuclear-containing fraction when preparing samples, and compare like fractions across samples.
Efficient DNA binding requires dimerization with another bHLH protein, but that functional interaction does not establish a visible dimer band. The supplied features list no disulfide bonds.
NEUROG2 is a nuclear transcription regulator involved in neurogenesis and differentiation. Compare samples prepared from matching cell contexts and nuclear-containing fractions so differences in sample composition do not obscure the comparison.
The predicted mass is 28.6 kDa, and only one isoform is listed. An additional band cannot be assigned to an isoform or a specific modification from these features alone; verify its identity before interpreting it as NEUROG2.
NEUROG2 is annotated as nuclear. Include a nuclear-containing fraction when preparing samples, and compare like fractions across samples.
Efficient DNA binding requires dimerization with another bHLH protein, but that functional interaction does not establish a visible dimer band. The supplied features list no disulfide bonds.
NEUROG2 is a nuclear transcription regulator involved in neurogenesis and differentiation. Compare samples prepared from matching cell contexts and nuclear-containing fractions so differences in sample composition do not obscure the comparison.
The predicted mass is 28.6 kDa, and only one isoform is listed. An additional band cannot be assigned to an isoform or a specific modification from these features alone; verify its identity before interpreting it as NEUROG2.
Boster reagents
NEUROG2 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-NGN2 NEUROG2 Antibody
Cat # A10153
Real WB data
Anti-Neurogenin 2 Rabbit Monoclonal Antibody
Cat # M10153
Two the supplier anti-NEUROG2 antibodies are listed, both with stated Human, Mouse, and Rat reactivity and WB images. A10153 is shown with mouse small intestine lysate at 1 and 2 μg/mL; M10153 is shown with SH-SY5Y lysate. The supplied WB evidence is limited to these examples.
Which to pick: Choose A10153 for a WB example using mouse small intestine lysate, or M10153 for one using SH-SY5Y lysate. Both have WB images, but these examples alone do not establish performance across all listed species.
Source: BosterBio NEUROG2 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q9H2A3.
- Human Protein Atlas. NEUROG2 tissue expression.
- PMC12836837 — target-verified WB comparison
- PMC7081927 — target-verified WB comparison
- PMC12476790 — target-verified WB comparison