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Product Info Summary
| SKU: | A10153 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-NGN2 NEUROG2 Antibody
SKU/Catalog Number
A10153
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NGN2 NEUROG2 Antibody (Catalog # A10153). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NGN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-NGN2 NEUROG2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10153)
Host
Rabbit
Contents
NGN2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal NGN2 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human NGN2. The immunogen is located within the last 50 amino acids of NGN2.
Cross-reactivity
NGN2 antibody is predicted to not cross-react with neurogenin 1.
Reactive Species
A10153 is reactive to NEUROG2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
28.6 kDa
Background of NEUROG2
Neurogenin-2 (NGN2) is a neural-specific basic helix-loop-helix (bHLH) transcription factor that can specify a neuronal fate on ectodermal cells and is expressed in neural progenitor cells within the developing central and peripheral nervous systems. NGN2 is thought to work with Nurr1 to play a role in the differentiation and survival of midbrain dopaminergic neurons. It has also been suggested for use in human embryonic neural progenitors as a graft for spinal cord injuries
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10153 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NGN2 antibody can be used for detection of NGN2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NGN2 in mouse small intestine tissue lysate with NGN2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of NGN2 in human brain tissue with NGN2 antibody at 20 μg/mL.
Specific Publications For Anti-NGN2 NEUROG2 Antibody (A10153)
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