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- Table of Contents
Facts about Histone-lysine N-methyltransferase NSD3.
H3'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while'Lys-27' is a mark for transcriptional repression. .
Human | |
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Gene Name: | NSD3 |
Uniprot: | Q9BZ95 |
Entrez: | 54904 |
Belongs to: |
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class V-like SAM-binding methyltransferase superfamily |
Histone-lysine N-methyltransferase NSD3
Mass (kDA):
161.613 kDA
Human | |
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Location: | 8p11.23 |
Sequence: | 8; NC_000008.11 (38269704..38382272, complement) |
Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung.
Nucleus. Chromosome.
Boster Bio is the right place to go if you are looking for high-affinity primary antibodies against NSD3. This antibody reacts with Humans and Rats. It is therefore suitable for use across a range of applications. For more information, please read on. Boster Bio is an industry leader in high-affinity prim antibodies. If you're interested to purchase an antibody for NSD3, please refer to its catalog number, A32443.
The Anti-NSD3 Marker in BoSTER Bio is used to identify the fusion protein NSD3-NUT. NSD3 belongs to the Nuclear SET domain-containing (NSD) family of proteins. It is known that it interacts via its ET domain with BRD4 and is essential for BRD4NUT function. A knockdown of full length NSD3 causes differentiation loss in 797TRex cells.
BoSTER Bio Anti-NSD3 markers are composed of rabbit IgG. It is not intended to be used for diagnostic purposes. Boster bio's AntiNSD3 Marker is monoclonal anti-NSD3 antibody. It reacts to the proteins expressed by Rat, Mouse, Human cells.
NSD3NUT, which is found in 1221 cell lines, may function in the same way as BRD-NUT. Knockdown NSD3NUT decreased the proliferation of 1221 cells, while wild-type NSD3 was not affected by this. These cells were derived directly from the skin of a patient. The immunoblots on TC-797 cells revealed that NSD3NUT's gene expression level was determined.
This antibody targets BRD4. Both proteins are critical components of BRD4-NUT. BRD4 can modify chromatin. Knockdown and induction of differentiation in 1221 cell lines results in a reduction of proliferation. JQ1 inhibits NSD3 activities and represses BRD4.
NSD3 interacts with a variant of the histone protein, macroH2A1. The gene is an transcription factor that represses transcript genes and participates with the stabilization X chromosome. It also regulates cell differentiation and growth and has been associated to many types of cancer. The molecular mechanisms underlying NSD3's regulation are not well understood.
It is difficult to determine the function of NSD3, which is a poorly differentiated tumor. It has been shown to create focal regions, but its exact function is not known. However, RNA sequence results show that the RNA-sequencing results indicate that it spans NSD3's junction with NUT. These data show that NSD3 is functionally interrelated to NUT. This interaction may be critical to NMC cell differentiation.
High-affinity primary antibodies that recognize the human nuclear receptor-binding domain protein (NSD3) can be used to recognize the full NSD3 sequence. This HMTase belongs to a group of three HMTases which play crucial roles in the integrity and maintenance of chromatin. They are associated with a number of cancers, and have been implicated recently in a wide variety of human diseases. The NSD3 protein is located in the Lysine Degradation pathway and plays a key role in activating the retinoic Acid Receptor.
Primary antibodies with high affinity have been generated from human immune systems using this gene. They are more receptive to HA than the corresponding IgM. It is possible that memory B cells express antibodies that have affinity maturation to HA. Alternatively, anti-influenza antigens HA antibodies were discovered from the NSD3 genome. These results demonstrate that antibodies can target antigens using multiple structural strategies.
Next-generation sequencing (antibodies) has made it possible for clonal and germ line progenitors to be reconstructed. These sequences are often different from the sequences of true, unmutated ancestors. The NSD3 marker allows for the identification of mutations at the VL, VH gene segments. However the original VLJLJH and VHDJH junctional DNA sequences remain unclear. These antibodies also increase shape complementarity at the VH/HEL interface.
Next-generation sequencing and crystal structures of affinity-matured antibodies have paved the way for a better understanding of the evolution of the immune repertoire. This technology can also help to identify the evolutionary pathways for antibodies. It has allowed reconstruction of antibody lineages as a response to viral pathogens. These results are a landmark in the study affinity-matured antibody research.
R&D Systems' high-affinity polyclonal antibodies are highly effective at recognizing any antigen because of their high-affinity. The antibodies can bind to targets of different specificities and have a wide range of KD values as well as equilibrium dissociation constants. The polyclonal antibodies can be used to detect specific biomolecules and measure changes in their levels.
There has been evidence that the NSD3 proteins is associated with breast cancer and other types of cancers. This study also examined NSD3's role in lung cancer development, and its treatment. Despite the importance this gene plays in breast cancer, we still don't know much about the NSD3 protein. These results showed that NSD3 was implicated in the pathogenesis and progression of the disease.
The quality of staining depends on the concentration, time incubation, and the diluent. The more stable the temperature and time of incubation, the better the signal quality. High-affinity antibodies should be used in higher concentrations to achieve penetration of the target. The resulting staining is far more intense than that of low affinity antibodies.
PMID: 11549311 by Stec I., et al. WHSC1L1, on human chromosome 8p11.2, closely resembles WHSC1 and maps to a duplicated region shared with 4p16.3.
PMID: 11374904 by Angrand P.-O., et al. NSD3, a new SET domain-containing gene, maps to 8p12 and is amplified in human breast cancer cell lines.