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- Table of Contents
OASL / 2'-5'-oligoadenylate synthase-like protein · Western blot design guide
Design a Western Blot for OASL
Real validated OASL Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-OASL WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
OASL Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~59.2 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Bone marrow (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Acetylated | |
| Caveat | Alternative isoforms | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 3 isoform(s) |
Section 1
Real Curated OASL Western Blot Protocols
The A04123-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Target-positive lysate and matched negative control (standard starting point) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A04123-1; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected OASL Western Blot Band Size?
OASL has a predicted mass of 59.2 kDa; annotated isoforms could affect band patterns, but their migration has not been demonstrated here.
| Single band near 59.2 kDa | Consistent with the predicted OASL sequence; identity requires confirmation |
| Discrete band below 59.2 kDa | Could reflect an alternative isoform; its size is not supplied |
| Discrete band above 59.2 kDa | Identity and reason for altered migration are not established |
| Several discrete bands | The three annotated isoforms are possible contributors, but their migration is unverified |
💡Expected OASL appearanceUniProt predicts 59.2 kDa for OASL, but no empirical band size is supplied; confirm any band near that position with an OASL-specific control.
| UniProt predicted mass | 59.2 kDa is the sequence-based reference, not a measured band position |
| Isoform p56 | An annotated splice isoform whose apparent band size is not supplied |
| Isoform p30 | An annotated splice isoform that may differ in size; migration is unverified |
| Isoform 3 | An annotated splice isoform whose relative size and migration are unknown |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | OASL abundance in the sample is unknown | Check OASL expression and include a validated positive lysate |
| Band higher than expected | The reason for migration above 59.2 kDa is not established | Check the size marker and verify band identity with OASL depletion |
| Band lower than expected | An alternative isoform is possible, but its mass is not supplied | Check antibody epitope coverage and verify identity with OASL depletion |
| Multiple bands | Three splice isoforms are annotated, but distinct bands are unverified | Compare isoform expression and test band identity with OASL depletion |
| Weak or no signal | OASL abundance or recovery from nuclear and cytoplasmic material may be low | Check expression, lysate recovery, and a validated positive control |
Experiment evidence
Western blot images showing OASL band
A04123-1 · OASL
A04123-1 · OASL
A04123 · OASL
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for OASL Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for OASL in Western blot, you can use bone marrow lysate, which HPA lists as a medium positive tissue.
Positive control: Bone marrow (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: HPA reports medium signal in bone marrow and no detected signal in adipose tissue, so tissue controls are feasible but the positive band may be modest.
HPA tissue expression evidence for OASL
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Bone marrow | hematopoietic cells | Medium | Protein (IHC) | HPA → |
| Colon | endothelial cells | Medium | Protein (IHC) | HPA → |
| Oral mucosa | squamous epithelial cells | Medium | Protein (IHC) | HPA → |
| Placenta | trophoblastic cells | Medium | Protein (IHC) | HPA → |
| Skin | epidermal cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Appendix | glandular cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
| Caudate | glial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced OASL Western Blot Tips
Deeper troubleshooting and optimisation questions for OASL, answered from its protein features.
How should OASL band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Which OASL isoforms could produce different bands?
Isoforms · UniProt lists p56, p30, and isoform 3. Relative to the canonical sequence, p30 replaces residues 220–255 and lacks 256–514; isoform 3 lacks 220–349. These sequence differences could affect migration, but the features do not establish visible bands.
Check the antibody epitope against the listed sequences. Canonical residues 256–514 are absent from p30, while residues 220–349 are absent from isoform 3. These are canonical UniProt coordinates; antibody or paper numbering may differ.
Check whether the antibody recognizes each isoform before combining band signals. Canonical residues 1–219 precede both listed alternative sequence regions; an epitope there could recognize all three, subject to antibody validation. Quantify distinct bands separately when isoform identity is uncertain.
Check the antibody epitope against the listed sequences. Canonical residues 256–514 are absent from p30, while residues 220–349 are absent from isoform 3. These are canonical UniProt coordinates; antibody or paper numbering may differ.
Check whether the antibody recognizes each isoform before combining band signals. Canonical residues 1–219 precede both listed alternative sequence regions; an epitope there could recognize all three, subject to antibody validation. Quantify distinct bands separately when isoform identity is uncertain.
Can OASL acetylation explain a band shift?
PTM · UniProt lists N-acetylalanine at position 2 in canonical numbering. This feature alone does not demonstrate a visible band shift or explain a difference between apparent and predicted mass.
Does this guide establish induction of OASL?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for OASL?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A04123-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should OASL be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Should OASL migrate exactly at 59.2 kDa?
Interpretation · 59.2 kDa is the predicted mass for the 514-residue canonical sequence. No observed Western blot band is supplied, so use it as a reference, not an expected apparent mass or evidence of a shift.
First compare its detection with the antibody epitope and the p30 and isoform 3 sequence changes. The supplied features do not identify an observed band, so migration alone cannot establish its isoform or cause.
First compare its detection with the antibody epitope and the p30 and isoform 3 sequence changes. The supplied features do not identify an observed band, so migration alone cannot establish its isoform or cause.
Boster reagents
OASL Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-OASL Antibody (C-term)
Cat # A04123-1
Real WB data
Anti-OASL Antibody
Cat # A04123
Both listed anti-OASL antibodies have Western blot images: A04123-1 detects OASL in HL60 lysate, and A04123 is shown with mouse bladder tissue lysate at 1 μg/ml. These examples document specific tested samples, not broad validation across every listed species.
Which to pick: For an HL60 sample, consider A04123-1, listed for human reactivity. For mouse bladder tissue, A04123 has a matching WB example and is listed for human, mouse, and rat reactivity. The supplied evidence does not show WB results for every listed species.
Source: BosterBio OASL gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q15646.
- Human Protein Atlas. OASL tissue expression.
- PMC8905001 — target-verified WB comparison
- PMC10459088 — target-verified WB comparison
- PMC12804755 — target-verified WB comparison