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Product Info Summary
| SKU: | A04123 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-OASL Antibody
SKU/Catalog Number
A04123
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-OASL Antibody (Catalog # A04123). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
OASL antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-OASL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04123)
Host
Rabbit
Contents
OASL Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
OASL antibody was raised against a 17 amino acid peptide near the amino terminus of human OASL. The immunogen is located within the first 50 amino acids of OASL.
Cross-reactivity
OASL antibody is human, mouse and rat reactive.
Reactive Species
A04123 is reactive to OASL in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
59.2 kDa
Background of OASL
OASL (2'-5'-oligoadenylate synthetase-like), also known as p59OASL or TRIP14 (thyroid receptor-interacting protein 14), is a 514 amino acid protein that exists as two alternatively spliced isoforms, designated p56 and p30, and contains two ubiquitin-like domains (1). It is widely expressed in a variety of tissues and interacts with the ligand binding domain of the thyroid receptor (TR) and is able to bind double-stranded RNA and DNA, possibly playing a role in RNA degradation and the overall inhibition of protein synthesis (2-3). Methyl CpG-binding protein 1 (MBD1), which functions as a transcriptional repressor, was identified as a strong p59 OASL interactor (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04123 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
OASL antibody can be used for detection of OASL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 2.5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of OASL in mouse bladder tissue lysate with OASL antibody at 1 μg/ml.
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Immunocytochemistry of OASL1 in HepG2 cells with OASL1 antibody at 2.5 μg/mL.
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Immunofluorescence of OASL1 in HepG2 cells with OASL1 antibody at 20 μg/mL.
Specific Publications For Anti-OASL Antibody (A04123)
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