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- Table of Contents
PERM1 / PGC-1 and ERR-induced regulator in muscle protein 1 · Western blot design guide
Design a Western Blot for PERM1
Source-linked PERM1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-PERM1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled October 2026 · For research use; verify linked source records and product datasheet before use
PERM1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~81.4 kDa | |
| Observed band | 75 kDa | |
| Gel | 5–20% (catalog A15642) | |
| Positive control | Heart muscle (IHC candidate; verify WB) +1 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | Band identity controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 3 isoform(s) |
Section 1
Source-Linked PERM1 Western Blot Protocol Options
The A15642 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human MCF-7, human Caco-2, human 293T (catalog A15642) |
| Gel % | 5–20% (catalog A15642) |
| Load | 30 ug; reducing conditions (catalog A15642) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A15642) |
| Membrane | nitrocellulose membrane (catalog A15642) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A15642) |
| Primary antibody | A15642 · 0.5 μg/mL (catalog A15642) |
| Primary incubation | overnight at 4°C (catalog A15642) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A15642) |
| Secondary incubation | 1.5 hour at RT (catalog A15642) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A15642) |
| Detection | ECL (catalog A15642) |
Section 2
What Is the Expected PERM1 Western Blot Band Size?
PERM1 is predicted at 81.4 kDa, while antibody blots show about 75 kDa; the cause of this difference is not established.
| Band near 75 kDa | empirical PERM1 band reported in whole-cell lysates; confirm identity with controls |
| Band near 81.4 kDa | near the predicted mass; identity requires validation |
| Several discrete bands | isoforms 1, 2, and 3 are possible contributors, but distinct migration is unestablished |
| Weak nuclear-fraction band without PPARGC1A | nuclear localization is reported in the presence of PPARGC1A |
💡Expected PERM1 appearancePERM1 has a predicted mass of 81.4 kDa, while antibody blots report a band near 75 kDa; the difference is unexplained, so confirm band identity with knockdown or an independent antibody.
| UniProt predicted mass | 81.4 kDa is predicted, while 75 kDa was observed; the cause of the difference is unknown |
| Isoform 1 | may migrate differently from other isoforms; its mass is not supplied |
| Isoform 2 | may migrate differently from other isoforms; its mass is not supplied |
| Isoform 3 | may migrate differently from other isoforms; its mass is not supplied |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | PERM1 abundance in the tested lysate may be low | check expression and use a positive lysate control |
| Band higher than expected | the extra band has no established PERM1 size modifier | test specificity by PERM1 knockdown or an independent antibody |
| Band lower than expected | the reported 75 kDa band differs from the 81.4 kDa prediction for an unknown reason | compare with the reported band and verify identity by PERM1 knockdown |
| Multiple bands | alternative splicing yields isoforms 1, 2, and 3, though their migration is unknown | verify band identity by knockdown and check antibody recognition of each isoform |
| Weak or no signal | PERM1 may be unevenly distributed between cytoplasm and nucleus | compare whole-cell and fractionated lysates and check PPARGC1A status |
| Fragments below expected size | degradation or nonspecific binding; no cleavage feature is listed | prepare fresh lysate with protease inhibitors and verify bands by knockdown |
Experiment evidence
Western blot images showing PERM1 band
A15642 · PERM1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PERM1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for PERM1 in Western blot, you can use heart muscle lysate, which HPA scores as high for PERM1 expression.
Positive control: Heart muscle (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: PERM1 is intracellular, and its tissue-dependent expression makes sample choice important.
HPA tissue expression evidence for PERM1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Heart muscle | cardiomyocytes | High | Protein (IHC) | HPA → |
| Skeletal muscle | myocytes | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Appendix | glandular cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced PERM1 Western Blot Tips
Deeper troubleshooting and optimisation questions for PERM1, answered from its protein features.
How should PERM1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
How could PERM1 isoforms affect the bands I detect?
Isoforms · Three isoforms are listed. Isoform 2 lacks canonical residues 1–732, and isoform 3 replaces canonical residues 1–122 with MEPRGGGS. Antibodies recognizing those regions may detect the isoforms differently. These sequence changes alone do not establish where any isoform runs on a blot.
No isoform-specific apparent masses are supplied. The 75 kDa observation alone cannot distinguish isoforms 1, 2, and 3. Use an antibody epitope that discriminates their annotated sequence differences, or another identity check, before assigning the band.
No isoform-specific apparent masses are supplied. The 75 kDa observation alone cannot distinguish isoforms 1, 2, and 3. Use an antibody epitope that discriminates their annotated sequence differences, or another identity check, before assigning the band.
Do annotated modifications explain an unexpected PERM1 band?
PTM · The supplied features list no modified residues or glycosylation sites. They therefore provide no specific modification to assign to an unexpected band. Compare its antibody recognition with the three annotated isoform sequences and verify band identity before interpreting it.
Does this guide establish induction of PERM1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for PERM1?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A15642 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should I quantify PERM1 across samples?
Quantitation · Use the same cellular fraction and identify which band or bands the antibody detects before comparing signal. PERM1 has three annotated isoforms, and its reported localization includes both cytoplasm and nucleus; a change in band or fraction signal need not represent a change in total PERM1.
Why might PERM1 run near 75 kDa instead of 81.4 kDa?
Interpretation · The supplied apparent band is 75 kDa, while the predicted mass is 81.4 kDa. The listed features do not establish why they differ. Check band identity and antibody specificity before assigning the difference to an isoform or modification.
Check the antibody epitope against the isoform sequences. A canonical epitope within residues 1–122 is changed in isoform 3 and absent from isoform 2. An epitope within residues 123–732 is absent from isoform 2. Coordinates here refer to the supplied canonical UniProt sequence.
The supplied location annotation lists PERM1 in the cytoplasm and nucleus and notes nuclear localization in the presence of PPARGC1A. When comparing conditions, keep the sampled cell fraction consistent. This annotation does not establish a change in total PERM1 abundance.
Check the antibody epitope against the isoform sequences. A canonical epitope within residues 1–122 is changed in isoform 3 and absent from isoform 2. An epitope within residues 123–732 is absent from isoform 2. Coordinates here refer to the supplied canonical UniProt sequence.
The supplied location annotation lists PERM1 in the cytoplasm and nucleus and notes nuclear localization in the presence of PPARGC1A. When comparing conditions, keep the sampled cell fraction consistent. This annotation does not establish a change in total PERM1 abundance.
Boster reagents
PERM1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-PERM1 Antibody Picoband®
Cat # A15642
A15642 is a rabbit polyclonal anti-PERM1 antibody with a supplied Western blot image from human MCF-7, Caco-2, and 293T whole-cell lysates. The reported band is approximately 75 kDa versus an expected 85 kDa; no independent validation is supplied.
Which to pick: A15642 is the only listed option. Its stated reactivity is human, and its Western blot image uses human cell lysates at 30 µg per lane with antibody at 0.5 µg/mL. Consider the 75 versus 85 kDa band discrepancy when interpreting results.
Source: BosterBio PERM1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q5SV97.
- Human Protein Atlas. PERM1 tissue expression.
- PMC6480336 — target-verified WB comparison
- PMC13461155 — target-verified WB comparison
- PMC9418182 — target-verified WB comparison