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Product Info Summary
| SKU: | A15642 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-PERM1 Antibody Picoband®
SKU/Catalog Number
A15642
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PERM1 Antibody Picoband® catalog # A15642. Tested in WB, ICC/IF, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PERM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15642)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PERM1 recombinant protein (Position: R69-N763). Human PERM1 shares 50.1% amino acid (aa) sequence identity with mouse PERM1.
Reactive Species
A15642 is reactive to PERM1 in Human
Observed Molecular Weight
75 kDa
Calculated molecular weight
81.4 kDa
Background of PERM1
PERM1 is predicted to play a role in glucose and lipid metabolism, energy transfer, and muscle contraction. In mouse muscle cells, Perm1 functions downstream of peroxisome proliferator-activated receptor-gamma coactivator-1 and estrogen-related receptors and regulates a subset of Pgc1- and Err-dependent genes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15642 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human MCF-7 whole cell, human Caco-2 whole cell, human 293T whole cell
ICC/IF: U2OS cell
FCM: MCF-7 cell
Validation Images & Assay Conditions
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Western blot analysis of PERM1 using anti-PERM1 antibody (A15642).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PERM1 antigen affinity purified polyclonal antibody (Catalog # A15642) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PERM1 at approximately 75 kDa. The expected band size for PERM1 is at 85 kDa.
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IF analysis of PERM1 using anti-PERM1 antibody (A15642) and anti-Beta Tubulin antibody (M01857-3).
PERM1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PERM1 Antibody (A15642) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127)(B) and DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133)(D) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI(C). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Flow Cytometry analysis of MCF-7 cells using anti-PERM1 antibody (A15642).
Overlay histogram showing MCF-7 cells stained with A15642 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PERM1 Antibody (A15642, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-PERM1 Antibody Picoband® (A15642)
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