Perilipin-2 Antibodies

Perilipin-2 (PLIN2)

May be involved in development and maintenance of adipose tissue. .

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Gene Information

Human
Gene Name:	PLIN2
Uniprot:	Q99541
Entrez:	123

Family

Belongs to:
perilipin family

Alternative Names

ADFP; Adipophilin; Adipose differentiation-related proteinadipophilin; ADRP; MGC10598; perilipin 2; Perilipin2; Perilipin-2; PLIN2

Molecular Weight

Mass (kDA):
48.075 kDA

Genomic Context

Human
Location:	9p22.1
Sequence:	9; NC_000009.12 (19108391..19127606, complement)

Tissue Specificity

Milk lipid globules.

Subcellular Localizations

Membrane; Peripheral membrane protein. Lipid droplet.

Diseases

• Steatosis
• Lymphoproliferative Disorder Of The Skin
• Liver Diseases
• Malignant Neoplasms
• Fatty Liver
• Diabetes Mellitus
• Insulin Sensitivity
• Obesity
• Inflammation
• Sclerosis
• Malignant Neoplasm Of Breast
• Endoplasmic Reticulum Stress
• Insulin Resistance

• Nonalcoholic Fatty Liver Disease
• Fibrosis
• Pain
• Mammary Neoplasms
• Colorectal Cancer
• Uterine Fibroids
• Muscle Spasticity

Pathways

• Localization
• Lipid Storage
• Secretion
• Lactation
• Cell Migration
• Mitosis
• Oxidative Phosphorylation
• Hepatocyte Proliferation
• Spermatogenesis
• Regulation Of Hepatocyte Proliferation

• Cell Growth
• Regulation Of Cell Growth
• Fatty Acid Oxidation
• Triglyceride Homeostasis
• Cholesterol Homeostasis

PTMs

• Oxidation

• Phosphorylation

• Cleavage

Research Areas

• Cancer
• Hypoxia
• Lipid and Metabolism
• Lipid Droplets
• Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PLIN2

Boster Bio Antibodies - Best Uses Of The PLIN2 Marker

Boster Bio is a leader in immunology tools and offers an exclusive suite of PLIN2 antibodies. Whether you're a biologist or a scientist looking for new ways to test cell types and proteins, Boster has an antibody for you. The PLIN2 molecule is a highly specific cytokine that can be used to target specific targets in cells and tissues. Developed in the UK, this antibody has a unique fluorescent signal that can be used to detect cellular functions.

IHC

IHC uses of the PLIN2 marker have been developed to study the role of Plin2 in LD hydrolysis. The PLIN2 gene encodes a protein that plays a role in tissues and is a marker of cardiomyocytes in particular. Gross et al. (2010) mapped the Plin2 gene to chromosome 9p22.1 by aligning the gene sequence to the genome.

In this study, PLIN2 expression was detected in human ccRCC tissues and cell lines. Functional analysis indicated that PLIN2 expression was elevated in RCC tissues and was associated with various clinicopathological factors. Interestingly, high PLIN2 expression was associated with favorable prognosis. In a multivariate analysis, high PLIN2 expression was a significant predictor of overall survival. In addition, knockdown of PLIN2 significantly promoted ccRCC cell proliferation, invasion, and migration.

Initially, PLIN2 was considered an RNA transcript that functions in the differentiation of adipocytes. It is widely expressed in tissues and has a direct correlation with intracellular lipids. Increased expression of PLIN2 has been found in several fat accumulation disorders, including cancers. Several types of tumors overexpress PLIN2 and are generally clear-cell in histology. It has been used to monitor prognosis in multiple types of tumor.

In addition to analyzing PLIN2 in pRCc, chRCc, and RO tissues, LYMP3 was recently identified as a potential biomarker in pRCC and chRCc. The PLIN2 marker stained more than ten percent of cells in ccRCC tumors and ninety percent of benign kidney tissue. Furthermore, PLIN2 was highly specific for ccRCC and was 100% sensitive and specific for pRCC.

Immunofluorescence

The PLIN2 marker has been identified as a potent marker in cancer. When elevated levels of this gene are detected in cancer cells, they inhibit the activation of CD8+ T cells and increase the activity of Foxp3+ Tregs. This marker is found in cancer cells, macrophages, and DCs, and is highly correlated with the presence of tumor-infiltrating lymphocytes.

The PLIN2 gene is strongly related to other important markers of immune cells, including CD8+ T cells, general T cells, and B cells. It also co-localizes with other immune cells such as Th27 and regulatory T cells. PLIN2 has also been linked to immune infiltration of TMEs and HNSCCs. Its expression levels are closely linked to the level of inflammation and tumor growth.

The expression of PLIN2 decreased with increasing tumor T stage and grade. The PLIN2 gene may be downregulated after transcriptional activation alongside the dedifferentiation process in ccRCC. PLIN2 may act as an anti-oncogene and serve an important role in the progression of ccRCC. The PLIN2 gene is present in most cancer cells.

Studies have shown that overexpression of PLIN2 in COS-7 cells induces the accumulation of LD. Additionally, PLIN2 knockout mice are resistant to alcoholic steatosis, diet-induced obesity, and fatty liver disease. This marker is responsible for the accumulation of lipids in the liver by promoting the formation of LD and protecting TG from lipolysis.

Single-cell sequencing

The PLIN2 marker is highly related to several immune cells. It is strongly associated with CD8+ T cells, general T cells, B cells, and TAMs. Other immune cell markers are also highly correlated with PLIN2, including CD68 and Th27. Using single-cell sequencing, researchers have now been able to detect the PLIN2 marker in a variety of different tissues. Detailed results are now available to help physicians identify cancer.

We performed this analysis using the 10x Chromium platform library preparation kit. We obtained paired-end reads from different biopsies. To minimize false-positives, we filtered the reads with a read quality lower than 30. Next, we used the CellRanger Count software to align the reads to the GRCh38 reference genome. Our results are deposited in the Genomics archive and Gene Expression Omnibus.

The researchers also found an association between PLIN2 expression and the TNM stage of a lung tumor. This association was further analyzed using statistical methods. PLIN2 expression correlated with the development of postoperative metastasis, recurrence, and disease. In addition, high PLIN2 expression was also associated with a greater risk of postoperative disease. These findings confirm the prognostic value of PLIN2.

In addition to analyzing tumor cell genomics, we also tested organoids derived from patients. During organoid culture, tumor cells express OLFM4 and CA2 markers. The two genes remained highly expressed after the organoids were conditioned and cultured. In contrast, normal tissues retain their original tissue-derived organoids. The tumor organoids, on the other hand, display CNVs and point mutations.

Foxp3 + Tregs

The PLIN2 marker is a gene that regulates cell growth and metabolism. The gene has widespread applications in the scientific community. It is activated by the PPAR signaling pathway. Researchers have observed high expression levels of PLIN2 in most malignant melanoma cases. Additionally, overexpression of PLIN2 in breast cancer is associated with poorer prognosis. Although current research has been relatively superficial, it is possible that PLIN2 plays an important role in tumorigenesis and progression.

PLIN2 expression levels were significantly increased in ccRCC tissues, when compared to paired normal tissues. This marker also has an important role in tumorigenesis and may function as an oncogene. Further studies are needed to fully understand the functions of PLIN2.

PLIN2 has significant clinical implications in the study of ccRCC. It has biological functions and affects cell migration and invasion. Extensive data analysis has shown that high PLIN2 expression is associated with good prognosis. Moreover, a recent study indicated that PLIN2 knockdown increased cell proliferation, migration, and invasion. The study also found that knockdown of PLIN2 in ccRCC cells increased cell migration, invasion, and proliferative activity.

cBioPortal

The PLIN2 marker has several important roles in the immune system. This protein is highly related to several important markers of various immune cell types, including CD8+ T cells, general T cells, B cells, TAMs, and natural killer (NK) cells. It is also strongly related to immune infiltration in HNSCC and TME. Therefore, the PLIN2 gene is an excellent candidate marker for immune cell infiltration studies.

The LD level of PLIN2 was determined using single-cell sequencing of 96 oral squamous cell carcinoma cells. Then, PLIN2 was correlated with the number of T cells, the number of CD4+ T cells, and the presence of IL-23. Lastly, PLIN2 was related to the immune microenvironment of OSCC.