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- Table of Contents
PLIN2 antibody (PB10083) performs well in Flow Cytometry, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Flow Cytometry analysis of HEL cells using anti-ADFP/PLIN2 antibody (PB10083).
Overlay histogram showing HEL cells stained with PB10083 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADFP/PLIN2 Antibody (PB10083, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEL cells stained with PB10083 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADFP/PLIN2 Antibody (PB10083, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-ADFP/PLIN2 Antibody Picoband®
PLIN2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, WB |
Tested Applications
PLIN2 antibody was positively detected by WB in:
human HepG2 whole cell, human K562 whole cell, human JAR whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PLIN2 antibody was positively detected by FC in:
HEL cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Flow Cytometry(Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-ADFP/PLIN2 Antibody Picoband®
View all PLIN2 Antibodies
View all PLIN2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ADFP recombinant protein (Position: K226-Q418). Human ADFP shares 88.4% amino acid (aa) sequence identity with mouse ADFP.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48 kDa
Calculated Molecular Weight
48.1 kDa
Background of PLIN2
Adipose differentiation-related protein, also known as perilipin 2 (PLIN2), ADRP or adipophilin, is a protein which in humans is encoded by the ADFP gene. The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.
Cite This Product
Anti-ADFP/PLIN2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10083)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
90%
High Homology
Cow
87%
Moderate Homology
Dog
87%
Moderate Homology
Mouse
86%
Moderate Homology
Rabbit
86%
Moderate Homology
Sheep
86%
Moderate Homology
Rat
76%
Moderate Homology
Zebrafish
46%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PLIN2 antibody
Flow Cytometry (FC) protocol for PLIN2 antibody
Publications
Application:
Species:
PLIN2 exacerbates Allergic Rhinitis by inhibiting PINK1/Parkin-mediated mitophagy
Published: 2025-11-03
Journal: IMMUNOLOGY LETTERS
Application: IHC
Impact Factor: 2.80
Species: Mouse
PMID: 41192682
Zhishi Xiebai Guizhi Decoction modulates hypoxia and lipid toxicity to alleviate pulmonary vascular remodeling of pulmonary hypertension in rats
Published: 2024-12-19
Journal: Chinese Medicine
Application: WB
Impact Factor: 5.30
Species: Rat
PMID: 39696593
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Product Promise
We promise Anti-ADFP/PLIN2 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB10083
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026