DNA polymerase alpha catalytic subunit (POLA1)

Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1.

The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are originally extended by the polymerase alpha catalytic subunit and then transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively.