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- Table of Contents
POLD1 Antibodies
AND POLD1 ELISA kits, POLD1 Proteins
Many biological assays use antibodies to detect POLD1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with POLD1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop POLD1 antibodies...
Designing an IHC experiment for POLD1? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the POLD1 IHC GuidePOLD1 Infographic by Boster Bio
All POLD1 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA, IP, IF, IHC, ICC, WB
$370
Cat # A03720-2
100 μg/vial
Cat # A03720-2
100 μg/vial
Human, Mouse, Rat
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # M03720
100 μl/vial
Cat # M03720
100 μl/vial
POLD1 Overview
Facts about DNA polymerase delta catalytic subunit.
DNA polymerase delta catalytic subunit (POLD1)
As the catalytic part of the trimeric (Pol-delta3 complicated ) and tetrameric DNA polymerase delta complexes (Pol-delta4 complicated ), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and fix. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity.
Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433).
Gene Information
| Human | |
|---|---|
| Gene Name: | POLD1 |
| Uniprot: | P28340 |
| Entrez: | 5424 |
Family
| Belongs to: |
|---|
| DNA polymerase type-B family |
Alternative Names
CDC2 homolog; CDC2; DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125; DNA-directed polymerase delta 1; EC 2.7.7.7; POLD; polymerase (DNA directed), delta 1, catalytic subunit (125kD); polymerase (DNA directed), delta 1, catalytic subunit 125kDa
Molecular Weight
Mass (kDA):
123.631 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 19q13.33 |
| Sequence: | 19; NC_000019.10 (50384291..50418018) |
Tissue Specificity
Widely expressed, with high levels of expression in heart and lung.
Subcellular Localizations
Nucleus. Colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). After UV irradiation, recruited to DNA damage sites within 2 hours, independently on the cell cycle phase, nor on PCNA ubiquitination. This recruitment requires POLD3, PCNA and RFC1-replication factor C complex (PubMed:20227374, PubMed:22801543).
Diseases
- Neoplasms
- Malignant Neoplasms
- Carcinoma
- Cell Transformation, Neoplastic
- Malignant Neoplasm Of Breast
- Mammary Neoplasms
- Genomic Instability
- Malignant Paraganglionic Neoplasm
- Adenoma
- Thyroid Neoplasm
- Chromosomal Translocation
- Malignant Squamous Cell Neoplasm
- Mutation, Out-of-frame
Interacting Proteins
- PCNA
- POLD2
- POLD4
- TERF2IP
- WRNIP1
Pathways
- Dna Repair
- Dna Replication
- Cell Cycle
- Base-excision Repair
- Cell Cycle Arrest
- Double-strand Break Repair
- Mismatch Repair
- Cell Adhesion
- G1 Phase
- Aging
- Strand Displacement
- Keratinocyte Differentiation
- Tropism
PTMs
- Methylation
Research Areas
- Breast Cancer
- Core ESC-Like Genes
- DNA Polymerases
- DNA Repair
- Stem Cell Markers
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/POLD1
bosterbio.com/bosterbio-gene-info-cards/POLD1
References
PMID: 1722322 by Chung D.W., et al. Primary structure of the catalytic subunit of human DNA polymerase delta and chromosomal location of the gene.
PMID: 1542570 by Yang C.-L., et al. Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta.