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Product Info Summary
| SKU: | A04421 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DNA pol alpha Antibody
View all DNA polymerase alpha Antibodies
SKU/Catalog Number
A04421
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DNA pol alpha Antibody catalog # A04421. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA pol alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04421)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human RRS1. at AA rangle: 230-310
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04421 is reactive to POLA1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
165913 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04421 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Positive Control
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using DNA pol alpha Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For POLA1 (Source: Uniprot.org, NCBI)
Gene Name
POLA1
Full Name
DNA polymerase alpha catalytic subunit
Weight
165913 MW
Superfamily
DNA polymerase type-B family
Alternative Names
DNA polymerase alpha 1 catalytic subunit; DNA polymerase alpha catalytic subunit p180; DNA polymerase alpha catalytic subunit; DNA polymerase alpha p180 subunit; EC 2.7.7.7; p180; POLADKFZp686K1672; polymerase (DNA directed), alpha 1; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA directed), alpha; polymerase (DNA-directed), alpha (70kD) POLA1 NSX, POLA, VEODS, p180 DNA polymerase alpha 1, catalytic subunit DNA polymerase alpha catalytic subunit|DNA polymerase alpha catalytic subunit p180|polymerase (DNA directed), alpha 1, catalytic subunit|polymerase (DNA) alpha 1, catalytic subunit|polymerase (DNA-directed), alpha (70kD)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLA1, check out the POLA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNA pol alpha Antibody (A04421)
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No publications found for A04421
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Anti-DNA pol alpha Antibody
Question
Can you help my question with product A04421, anti-DNA pol alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04421 anti-DNA pol alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-24
Question
We are currently using anti-DNA pol alpha antibody A04421 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
K. Krishna
Verified customer
Asked: 2019-11-06
Answer
The anti-DNA pol alpha antibody (A04421) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-06
Question
Will anti-DNA pol alpha antibody A04421 work on dog WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Our lab technicians have not validated anti-DNA pol alpha antibody A04421 on dog. You can run a BLAST between dog and the immunogen sequence of anti-DNA pol alpha antibody A04421 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-31
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-DNA pol alpha antibody A04421. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-17
Question
We need to test anti-DNA pol alpha antibody A04421 on human cervix carcinoma for research purposes, then I may be interested in using anti-DNA pol alpha antibody A04421 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
The products we sell, including anti-DNA pol alpha antibody A04421, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-29
Question
I see that the anti-DNA pol alpha antibody A04421 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-12
Question
Is a blocking peptide available for product anti-DNA pol alpha antibody (A04421)?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
We do provide the blocking peptide for product anti-DNA pol alpha antibody (A04421). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-24
Question
Does anti-DNA pol alpha antibody A04421 work for WB with cervix carcinoma?
B. Wu
Verified customer
Asked: 2018-12-13
Answer
According to the expression profile of cervix carcinoma, POLA1 is highly expressed in cervix carcinoma. So, it is likely that anti-DNA pol alpha antibody A04421 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2018-12-13
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma using anti-DNA pol alpha antibody A04421. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-04-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-03
Question
Would A04421 anti-DNA pol alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Zhao
Verified customer
Asked: 2017-10-23
Answer
As indicated on the product datasheet, A04421 anti-DNA pol alpha antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-23
Question
Do you have a BSA free version of anti-DNA pol alpha antibody A04421 available?
L. Walker
Verified customer
Asked: 2016-02-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DNA pol alpha antibody A04421 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-02-29
Question
I was wanting to use your anti-DNA pol alpha antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
C. Zhao
Verified customer
Asked: 2015-12-22
Answer
You can see on the product datasheet, A04421 anti-DNA pol alpha antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-12-22
