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- Table of Contents
PSME1 / Proteasome activator complex subunit 1 · Western blot design guide
Design a Western Blot for PSME1
Source-linked PSME1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-PSME1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
PSME1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~28.7 kDa | |
| Observed band | ~29 kDa | |
| Gel | 12% (catalog A04638-2) | |
| Positive control | Appendix (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 3 isoform(s) |
Section 1
Source-Linked PSME1 Western Blot Protocol Options
The A04638-2 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human Raji, human MCF-7, human Jurkat cells, rat liver, rat spleen, mouse liver, mouse spleen (catalog A04638-2) |
| Gel % | 12% (catalog A04638-2) |
| Load | 30 ug; reducing conditions (catalog A04638-2) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A04638-2) |
| Membrane | nitrocellulose membrane (catalog A04638-2) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A04638-2) |
| Primary antibody | A04638-2 · 0.5 μg/mL (catalog A04638-2) |
| Primary incubation | overnight at 4°C (catalog A04638-2) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A04638-2) |
| Secondary incubation | 1.5 hour at RT (catalog A04638-2) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A04638-2) |
| Detection | ECL (catalog A04638-2) |
Section 2
What Is the Expected PSME1 Western Blot Band Size?
PSME1 is predicted at 28.7 kDa and observed at ~29 kDa; the cause of the small difference is not established.
| Band at ~29 kDa | Matches the empirical PSME1 band and its 28.7 kDa predicted mass |
| Single band near 29 kDa | The three annotated isoforms need not resolve into separate bands |
| Several bands | Isoforms 1, 2, and 3 are possible contributors, but their migration is not supplied |
| Higher-mass band under native conditions | Could reflect a PSME1-containing proteasome activator assembly |
💡Expected PSME1 appearancePSME1 is predicted at 28.7 kDa and observed at ~29 kDa on reducing Western blots; confirm band identity with appropriate antibody and sample controls.
| Predicted PSME1 mass | Places the unmodified sequence near 28.7 kDa, consistent with the observed ~29 kDa band |
| Isoform 1 | Its individual mass and migration are not supplied |
| Isoform 2 | Alternative splicing could change size, but its mass and migration are not supplied |
| Isoform 3 | Alternative splicing could change size, but its mass and migration are not supplied |
| Situation | Likely cause | Next action |
|---|---|---|
| Band higher than expected | A PSME1-containing assembly may persist if denaturation is incomplete | Repeat with fully denaturing preparation and compare with the ~29 kDa band |
| Band lower than expected | Its identity is unclear; isoform masses are not supplied | Check antibody specificity and compare with a positive-control lysate |
| Multiple bands | Annotated isoforms may contribute, but distinct migration is unverified | Compare with an independent PSME1 antibody or PSME1-depleted sample |
| Weak or no signal | PSME1 may be below detection in the tested lysate | Check loading and transfer, then run a positive-control lysate |
| Fragments below expected size | Band identity or sample integrity is uncertain | Check sample handling and confirm PSME1 identity with an independent antibody |
Experiment evidence
Western blot images showing PSME1 band
A04638-2 · PSME1
M04638-1 · PSME1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PSME1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for PSME1 in Western blot, you can use appendix tissue lysate, which HPA rates as high expression.
Positive control: Appendix (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA reports high expression in appendix and no detection in adipose tissue, supporting a tissue control pair.
HPA tissue expression evidence for PSME1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | glandular cells | High | Protein (IHC) | HPA → |
| Endometrium | glandular cells | High | Protein (IHC) | HPA → |
| Lymph node | non-germinal center cells | High | Protein (IHC) | HPA → |
| Small intestine | glandular cells | High | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Cervix | glandular cells | Not detected | Protein (IHC) | HPA → |
| Esophagus | squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
| Ovary | follicle cells | Not detected | Protein (IHC) | HPA → |
| Skeletal muscle | myocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced PSME1 Western Blot Tips
Deeper troubleshooting and optimisation questions for PSME1, answered from its protein features.
How should PSME1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could PSME1 isoforms produce different bands?
Isoforms · Three isoforms are listed. Relative to isoform 1, isoform 2 replaces residues 224–249, while isoform 3 replaces 224–233 and lacks 234–249. These UniProt coordinates describe sequence differences, not confirmed band positions. Check whether the antibody recognizes the altered region before assigning a band to an isoform.
The isoform differences involve the canonical sequence at residues 224–249. An antibody recognizing that region may detect the three isoforms differently. Compare its stated epitope with those UniProt coordinates; antibody or paper numbering may use a different convention.
The isoform differences involve the canonical sequence at residues 224–249. An antibody recognizing that region may detect the three isoforms differently. Compare its stated epitope with those UniProt coordinates; antibody or paper numbering may use a different convention.
Do the listed modifications explain additional PSME1 bands?
PTM · No modified residues or glycosylation sites are listed in the supplied features. Do not assign an additional band to a particular modification from this evidence alone.
Does this guide establish induction of PSME1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for PSME1 Western blot?
Transfer · Choose transfer conditions validated for a protein near 29 kDa and check transfer efficiency in that size range. The supplied PSME1 features do not specify a membrane, transfer time, or method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A04638-2 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should PSME1 bands be quantified?
Quantitation · Define the band region consistently around the observed 29 kDa signal and use the same region across samples. Because three isoforms are listed, a single band cannot establish which isoform contributes to the signal without an antibody that distinguishes them.
Why does PSME1 appear near 29 kDa?
Interpretation · The predicted mass is 28.7 kDa, and the reported apparent band is about 29 kDa. These approximate values agree; the supplied features do not establish a meaningful mass shift.
PSME1 forms a heterodimer with PSME2 in a hexameric ring and can form homoheptamers. A higher band alone does not identify either complex; interpret it in light of whether the sample preparation preserves assemblies.
PSME1 forms a heterodimer with PSME2 in a hexameric ring and can form homoheptamers. A higher band alone does not identify either complex; interpret it in light of whether the sample preparation preserves assemblies.
Boster reagents
PSME1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-PSME1 Antibody Picoband®
Cat # A04638-2
Real WB data
Anti-PSME1 Rabbit Monoclonal Antibody
Cat # M04638-1
Both anti-PSME1 antibodies list human, mouse, and rat reactivity and have WB images. A04638-2 shows an approximately 29 kDa band in the specified human cell and rat/mouse tissue lysates. M04638-1 has a brief Jurkat lysate caption.
Which to pick: Choose A04638-2 if you want a WB example with reported loading and antibody conditions across the listed samples. M04638-1 has a Jurkat lysate WB image, but its supplied caption gives no conditions or band size.
Source: BosterBio PSME1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q06323.
- Human Protein Atlas. PSME1 tissue expression.
- PMC10198961 — target-verified WB comparison
- PMC3854201 — target-verified WB comparison
- PMC11606656 — target-verified WB comparison