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- Table of Contents
RAF1 Antibodies
AND RAF1 ELISA kits, RAF1 Proteins
Many biological assays use antibodies to detect RAF1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with RAF1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop RAF1 antibodies...
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RAF1 Infographic by Boster Bio
All RAF1 Products
Product
Figures
Specificity
Applications
Price
Anti-Raf1 Antibody Picoband®
1 Q&As
Human, Mouse, Rat
Flow Cytometry, WB
$370
Cat # A00446-1
100 μg/vial
Cat # A00446-1
100 μg/vial
Human, Mouse, Rat
IHC, WB
$370
Cat # P00446-5
100 μl
Cat # P00446-5
100 μl
Anti-Raf1 Rabbit Monoclonal Antibody
3 Citations
Human, Mouse, Rat
IF, IHC, ICC, WB
$370
Cat # M00446-1
100 μl
Cat # M00446-1
100 μl
Human, Mouse, Rat
ELISA
$480
Cat # EKC1139
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1139
1 kit, containing one 96-well plate and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC2115
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2115
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC2116
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2116
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC2150
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2150
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC2244
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2244
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC2500
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2500
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC2641
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2641
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA, IF, IHC, WB
$380
Cat # A00446S296
100ul
Cat # A00446S296
100ul
Human, Monkey, Mouse, Rat
ELISA, IF, IHC, WB
$380
Cat # A00446S621-2
100ul
Cat # A00446S621-2
100ul
RAF1 Overview
Facts about RAF proto-oncogene serine/threonine-protein kinase.
RAF proto-oncogene serine/threonine-protein kinase (RAF1)
Serine/threonine-protein kinase that functions as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation begins a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2).
The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2- antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, leading to their activation.
Gene Information
| Human | |
|---|---|
| Gene Name: | RAF1 |
| Uniprot: | P04049 |
| Entrez: | 5894 |
Family
| Belongs to: |
|---|
| protein kinase superfamily |
Alternative Names
cRaf; c-Raf; c-Raf-1; CRAFraf proto-oncogene serine/threonine protein kinase; EC 2.7.11; EC 2.7.11.1; NS5; Proto-oncogene c-RAF; RAF proto-oncogene serine/threonine-protein kinase; RAF; Raf1; Raf-1; Raf-1Oncogene RAF1; v-raf-1 murine leukemia viral oncogene homolog 1; v-Raf-1
Molecular Weight
Mass (kDA):
73.052 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 3p25.2 |
| Sequence: | 3; NC_000003.12 (12583601..12664201, complement) |
Tissue Specificity
In skeletal muscle, isoform 1 is more abundant than isoform 2.
Subcellular Localizations
Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.
Diseases
- Neoplasms
- Malignant Neoplasms
- Noonan Syndrome
- Carcinoma
- Dwarfism
- Cell Transformation, Neoplastic
- Leukemia
- Hypertrophy
- Congenital Heart Defects
- Hypertrophic Cardiomyopathy
- Von Hippel-lindau Syndrome
- Cardiomyopathies
- Tissue Adhesions
Interacting Proteins
- AKT1
- BAD
- BRAF
- CCT3
- CDC25A
- CDC37
- CPS1
- HRAS
- HSP90AB1
- HSPA5
Pathways
- Pathogenesis
- Cell Proliferation
- Cell Cycle
- Cell Growth
- Localization
- Secretion
- Cell Adhesion
- Cell Differentiation
- Angiogenesis
- Methylation
- Cell Division
- Mapk Cascade
- Cell Death
PTMs
- Phosphorylation
- Dephosphorylation
- Methylation
- Ubiquitination
- Cleavage
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RAF1
bosterbio.com/bosterbio-gene-info-cards/RAF1
References
PMID: 3003687 by Bonner T.I., et al. The complete coding sequence of the human raf oncogene and the corresponding structure of the c-raf-1 gene.
PMID: 2993863 by Bonner T.I., et al. Structure and biological activity of human homologs of the raf/mil oncogene.
1 Publications of Boster RAF1 Antibodies & ELISA Kits
- PubMed ID: 27803850, The role of Dectin-1/Raf-1 signal cascade in innate immune of human corneal epit...
*More publications can be found for each product on its corresponding product page