RAF1 (NM_002880) Human Over-expression Lysate

Lysate of Raf-1 over-expression cell lines, WB control

Transient overexpression lysate of v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1)

Product Info Summary

SKU: LS005894
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1)

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Product Name

RAF1 (NM_002880) Human Over-expression Lysate

See all Raf-1 products

SKU/Catalog Number

LS005894

Size

100 µg

Description

Transient overexpression lysate of v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1)

Storage & Handling

Cite This Product

RAF1 (NM_002880) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005894)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for RAF1 (Source: Uniprot.org, NCBI)

Uniprot ID

P04049

Gene ID

5894

Gene Name

RAF1

Full Name

RAF proto-oncogene serine/threonine-protein kinase

Weight

73.052kDa

Superfamily

protein kinase superfamily

Alternative Names

cRaf; c-Raf; c-Raf-1; CRAFraf proto-oncogene serine/threonine protein kinase; EC 2.7.11; EC 2.7.11.1; NS5; Proto-oncogene c-RAF; RAF proto-oncogene serine/threonine-protein kinase; RAF; Raf1; Raf-1; Raf-1Oncogene RAF1; v-raf-1 murine leukemia viral oncogene homolog 1; v-Raf-1 RAF1 CMD1NN, CRAF, NS5, Raf-1, c-Raf Raf-1 proto-oncogene, serine/threonine kinase RAF proto-oncogene serine/threonine-protein kinase|C-Raf proto-oncogene, serine/threonine kinase|Oncogene RAF1|proto-oncogene c-RAF|raf proto-oncogene serine/threonine protein kinase|v-raf-1 murine leukemia viral oncogene homolog 1|v-raf-1 murine leukemia viral oncogene-like protein 1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on RAF1, check out the RAF1 Infographic

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In this infographic, you will see the following information for RAF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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