Reg4 Antibodies

Regenerating islet-derived protein 4 (Reg4)

Calcium-independent lectin displaying mannose-binding specificity and equipped to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic reactions of the gastrointestinal epithelium (By similarity).

. .

Gene Information

Mouse
Gene Name:	Reg4
Uniprot:	Q9D8G5
Entrez:	67709

Family

Belongs to:
No superfamily

Alternative Names

Gastrointestinal secretory protein; GISP; GISPREG-4; Reg IV; Reg4; regenerating gene type IV; regenerating islet-derived family, member 4; regenerating islet-derived protein 4; Regenerating islet-derived protein IV; REG-IV; RELP; RELPREG-like protein

Molecular Weight

Mass (kDA):
18.398 kDA

Genomic Context

Mouse
Location:	3|3 F2.2
Sequence:	3;

Diseases

• Malignant Neoplasms
• Neoplasms
• Adenocarcinoma
• Malignant Neoplasm Of Stomach
• Carcinoma
• Stomach Neoplasms
• Colorectal Cancer
• Colorectal Neoplasms
• Neoplasm Metastasis
• Carcinogenesis
• Adenoma
• Inflammatory Bowel Diseases
• Intestinal Diseases

• Malignant Neoplasm Of Pancreas
• Pancreatic Neoplasm
• Cell Invasion
• Malignant Tumor Of Colon
• Malignant Paraganglionic Neoplasm
• Metaplasia
• Neoplasm Invasiveness

Pathways

• Cell Growth
• Cell Proliferation
• Regeneration
• Localization
• Cell Adhesion
• Cell Death
• Secretion
• Cell Migration
• Insulin Secretion
• Cell Cycle Arrest
• Drug Resistance
• Fermentation

• Translation
• Cellular Localization
• Cell Cycle
• Regulation Of Cell Growth
• Regionalization
• Pathogenesis
• Epithelium Development

PTMs

• Phosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Reg4

Best Uses of the Reg4 Marker From Boster Bio

The REG4 gene is an important protein for many different types experiments. Boster Bio has created several assays that use this marker. These include Cell proliferation assays and Antibodies. Let's look at the best uses of this protein. Hopefully, you'll find this information useful. Here are some examples of biological uses for Reg4:

Boster Bio

Reg4 has been identified as a marker in the small intestinal enteroendocrine cells. This marker has been further identified to be a marker for deep-crypt secretory cells in the colon. DCS cells are crucial for maintaining stem cells in the colonic epithelial-crypt crypts and maintaining an environment that is niche-friendly.

Antibodies

New serology assays have been developed that measure the immune response against key proteins of Ebola virus (EVD) after antibodies were discovered using the REG4 marker by Boster Biology. These assays are built on a reference antibody in order to standardize and improve quantification. These critical agents were created in order to facilitate the development and use of serology assays.

Assay of cell proliferation

Reg4 in cell proliferation assays is especially useful for investigating bacterial interaction with colonic epithelial, and microbiome component cells. Reg4's presence or absence is known to have an impact on the host-microbiome interaction. This could have implications for the function and integrity of the mucosal barrier. The REG4 marker has been used in multiple studies to assess the role this lectin plays in regulation of colonic inflammation as well as the role microbiome components in mucosal function.

Reg4 plays a vital role in the intestinal inflammatory response. To examine the role of Reg4 in intestinal immunity, conditional knockout mice were generated whose expression was deleted in intestinal epithelial cells. Despite having a reduced body weight, these mice did not exhibit any phenotypes that are associated with inflammation. They also displayed significantly reduced expression of intestinal alkaline phosphatase and phospho-STAT3, two factors associated with inflammatory responses.

The REG4 marker originally was identified as a marker to identify enteroendocrine and small intestine cells in mice. It was later found to be a useful marker of deep crypt secretory cell (DCS) in the colon. These cells are essential for maintaining stem cell numbers, niche environments, and other functions.

Boster Bio formulated the assay for Reg4 protein. China researchers discovered that Reg4 protein increased EdU incorporation and enhanced organoids. Stattic, which is a STAT3 inhibitor, was also observed to block this effect. This study has provided valuable insights into the role that this gene may play in inflammatory intestinal disease. The REG4 gene may play a role regulating cancer risk in many types of cancers.

Interactions between microbiome and bacteria

Recent research suggests that REG4 may modulate host-microbiome interactions. Microbiome members in the faecal compartment and adherence of bacteria to epithelial surfaces were affected. These findings indicate that Reg4 status can have implications for mucosa function. Comparing microbiota cultures taken from different mice determines the composition of the microbiome.

Reg4 was shown to reduce the number of Eschericia, Staphylococcus and Proteus in a mouse model. It also increased Clostridium and Bifidobacterium. An in vitro bacteria intestinal cell binding assay confirmed this result. Reg4-deficient mice had less adherent bacteria than Regfl/fl mice. Additionally, adherent bacteria numbers decreased in mice with Reg4 deficiencies. The bacterial group with the greatest reduction was Staphylococcus. Bacteroides also required exogenous Reg4 protein in order to modulate adherence.

Previous research showed that Reg4 promotes inflammation of the intestinal mucosa. To investigate the role of Reg4 and its effect on this response, conditional knockout mouse models were created that deleted Reg4 expression in intestinal epithelial tissues. The mice that were not genetically modified did not have any adverse phenotypes. However, they had reduced body weight, decreased goblet cell numbers and proliferative crypt cells. They also had significantly reduced levels of intestinal alkaline phosphatase and phospho-STAT3 (inflammatory response markers).