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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin).|
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Human REG-4 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human REG-4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human REG4 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human REG-4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1289)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: D23-P158
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1289 is reactive to REG4 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
EK1289 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of REG4
Regenerating islet-derived protein 4 is a protein that in humans is encoded by the REG4 gene. It is mapped to 1p12. REG-4 gene contains 6 exons and that the exon structure is preserved among members of the REG gene family. This gene is able to maintain carbohydrate recognition activity in an acidic environment. REG-4 expression was significantly upregulated by inflammation and tissue injury associated with active Crohn disease and ulcerative colitis, and most of the colorectal tumors overexpressing REG-4 are mucinous tumors or neuroendocrine tumors.
|EK1289-CAP||Anti-Human REG4 Pre-coated 96-well strip microplate||1|
|EK1289-ST||Human REG4 Standard||2 vials, 10 ng/tube|
|EK1289-DA||Human REG4 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human REG-4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect REG4, Dilution ratio of 1:1, concentration in serum is 2-4ng/ml.
Some articles we found to cite concentrations of REG4 in samples: 22866070 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human REG-4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of REG4 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For REG4 (Source: Uniprot.Org, NCBI)
Regenerating islet-derived protein 4
Gastrointestinal secretory protein; GISP; GISPREG-4; Reg IV; Reg4; regenerating gene type IV; regenerating islet-derived family, member 4; regenerating islet-derived protein 4; Regenerating islet-derived protein IV; REG-IV; RELP; RELPREG-like protein REG4 GISP, REG-IV, RELP regenerating family member 4 regenerating islet-derived protein 4|REG-4|REG-like protein|gastrointestinal secretory protein|reg IV|regenerating gene type IV|regenerating islet-derived family, member 4|regenerating islet-derived protein IV*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on REG4, check out the REG4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for REG4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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